Topic
Buffer solution
About: Buffer solution is a research topic. Over the lifetime, 6948 publications have been published within this topic receiving 112440 citations. The topic is also known as: pH buffer & buffer.
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TL;DR: A conductometric biosensor for nitrite detection was developed using cytochrome c nitrite reductase extracted from Desulfovibrio desulfuricans ATCC 27774 cells immobilized on a planar interdigitated electrode by cross-linking with saturated glutaraldehyde vapour in the presence of bovine serum albumin, methyl viologen (MV), Nafion, and glycerol.
39 citations
TL;DR: The contents of the nine components in crude extracts of either AR or LR could easily be determined within 60 min by LC and within 20 min by CE, and the structures of the individual peaks in the LC chromatogram were identified by LC-MS.
Abstract: Angelicae Radix (AR) and Ligustici Rhizoma (LR) are both derived from the Umbelliferae plants and contain similar organic acids as their bioactive compounds. Nine of these organic acids, including nicotinic acid, protocatechuic acid, phthalic acid, folinic acid, p-hydroxybenzoic acid, folic acid, vanillic acid, caffeic acid, and ferulic acid were separated by HPLC and CE. Detection at 210 nm with a linear gradient containing 20 mM KH2PO4 (pH 3.5) and H2O-CH3CN in HPLC and with a buffer solution containing 10 mM LTAC, 2 mM Na2HPO4, 9 mM Na2B4O7(pH 9.56), and CH3CN in CE were found to be the most efficient eluents for this separation. The contents of the nine components in crude extracts of either AR or LR could easily be determined within 60 min by LC and within 20 min by CE. The structures of the individual peaks in the LC chromatogram were identified by LC-MS. The effects of buffers on the separation and validation of the two methods were examined.
39 citations
TL;DR: In this paper, the role of the phosphate buffer in the H 2 O 2 oxidative decomposition of 2,4,6-trichlorophenol (TCP), a benchmark pollutant, catalyzed by iron(III) tetrasulfophthalocyanine (FePcS), was established.
39 citations
TL;DR: A low-cost electrochemical flow-through cell is designed and fabricated to use in conjunction with a flow injection (FI) system and the recommended method has been applied to the determination of acetaminophen in 8 commercial pharmaceutical preparations.
39 citations
TL;DR: In this paper, the single ion activity coefficients of hydrogen and chloride ions in aqueous HCl solutions have been estimated at 25°C at concentrations up to 1 mol-kg−1, using potentiometric measurements with ion-selective electrodes and appropriate calibration procedures.
Abstract: The single ion activity coefficients of hydrogen and chloride ions in aqueous HCl solutions have been estimated at 25°C at concentrations up to 1 mol-kg−1, using potentiometric measurements with ion-selective electrodes and appropriate calibration procedures. Two methods are described for an internal calibration of the electrodes in the extended Debye–Huckel concentration range. The results are compared to the conventional pH calibration with external buffer solutions. Since the latter calibration method does not account for the liquid junction potential E
J which arises at the reference electrode, the resulting activity coefficients are quite different in HCl solutions of higher concentration. These differences between internal and external calibration decrease significantly, when a correction for E
J is introduced into the conventional pH calibration. Hence, in solutions of higher ionic strength the accuracy of the conventional pH electrode calibration using buffer solutions is very limited, when exact H+ activities are required. The consistency of the results indicates that the liquid junction potentials in the examined systems calculated by the Henderson/Bates approximation are of reasonable precision.
39 citations