Topic
Buffer solution
About: Buffer solution is a research topic. Over the lifetime, 6948 publications have been published within this topic receiving 112440 citations. The topic is also known as: pH buffer & buffer.
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TL;DR: The results indicate that the stacking efficiency is markedly improved with addition of phosphate buffer in the sample matrix, and depending on the nature of the analytes, the most effective stacking of these analytes occurs when the ratio of the conductivity of buffer electrolyte to that of sample matrix is in the range 1.4-1.2.
38 citations
TL;DR: In this paper, it was shown that the affinity-enhancing influence of divalent cations depends strongly on the precise nature of the cation used, with barium being far more effective than the conventionally used magnesium.
38 citations
TL;DR: Chromium(III) and Chromium(VI) have been speciated by capillary electrophoresis (CE) with on-column UV-Vis detection at 254nm and a negative potential as mentioned in this paper.
Abstract: Chromium(III) and Chromium(VI) have been speciated by capillary electrophoresis(CE) with on-column UV-Vis detection at 254nm and a negative potential. Chromium(III) has been converted to an anionic complex using ethylenediaminetetraacetate (EDTA) to be detected simultaneously with Cr(VI) by CE. A cationic surfactant, cetyltrimethylammonium bromide (CTAB), was added to a buffer solution of 10mM phosphate in order to reverse the electroosmotic flow (EOF) in the capillary. This results in a short analysis time and better peak shapes. The effect of the buffer pH over the range of 6 to 11 on the separation of each species has been observed. Nitrate and chromate have been nicely separated at a higher pH. Linear calibrations for chromate and a chromic EDTA complex were established over the concentration range of 50-200pg/nl with detection limits of 1.5pg/nl and 5pg/nl, respectively, using a 10mM phosphate buffer and a 0.5mM CTAB solution.
37 citations
TL;DR: In this article, a self-assembled 5-hydroxy-3-hexanedithiol-1,4-naphthoquinone (JUG thio) was selfassembled on gold and the surface coverage is 2.2 × 10 −10 ǫ cm −2, consistent with a dense monolayer.
37 citations
TL;DR: In this article, the spectroscopic behavior of catechin (5,7,3,4,tetrahydroxyflavan-3-ol) has been studied in the presence and the absence of air using UV-vis absorption spectrophotometry and fluorescence spectroscopy.
Abstract: The spectroscopic behavior of catechin (5,7,3',4'-tetrahydroxyflavan-3-ol), has been studied in the presence and the absence of air using UV-vis absorption spectrophotometry and fluorescence spectroscopy. The UV-vis absorption spectrum of catechin shows a very sharp and strong absorption maximum peak at 275 nm in deaerated water. New absorption maximum peaks appeared in aerated water, as well as in basic aqueous solution, caused by the oxidation of catechin. The absorbances in the UV-vis absorption spectrum of catechin decreased when the solution was left in the dark for a long time. The fluorescence emission spectrum of catechin after a long time period differs markedly from that in freshly prepared solution; the fluorescence maxia shifted as time passes after adding catechin to the solutions. When the deaerated basic catechin solutions were left in the dark for a long time, their fluorescence quantum yields were found to be nearly zero. This suggests that the oxidized catechin molecules were seen to have slowly undergone successive chemical reactions in basic buffer solution.
37 citations