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Buffer solution

About: Buffer solution is a research topic. Over the lifetime, 6948 publications have been published within this topic receiving 112440 citations. The topic is also known as: pH buffer & buffer.


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Journal ArticleDOI
TL;DR: P pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells, and thefluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH.
Abstract: We have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes.

102 citations

Journal ArticleDOI
06 Jul 2009-Langmuir
TL;DR: The level of stratification and the degree of layer intermixing can be controlled by varying the type and concentration of salt during SA-LbL assembly and the final microstructure in comparison with traditional LbL assemblies is found to be more stratified.
Abstract: We apply neutron reflectivity to probe the internal structure of spin-assisted layer-by-layer (SA-LbL) films composed of electrostatically assembled polyelectrolytes. We find that the level of stratification and the degree of layer intermixing can be controlled by varying the type and concentration of salt during SA-LbL assembly. We observe well-defined layer structure in SA-LbL films when deposited from salt-free solutions. These films feature 2-nm-thick bilayers, which are approximately 3-fold thicker than those in conventional LbL films assembled under similar conditions. The addition of a 10 mM phosphate buffer promotes progressive layer interdiffusion with increasing distance from the substrate. However, adding 0.1 M NaCl to the phosphate buffer solution restores the layer stratification. We also find that SA-LbL films obtained from buffer solutions are more stratified as compared to the highly intermixed layers seen in conventional LbL films from buffer. Our results yield new insights into the mechanism of SA-LbL assembly and the final microstructure in comparison with traditional LbL assemblies.

102 citations

Journal ArticleDOI
TL;DR: In this paper, a novel fluorescent sensor based on thiooxorhodamine B has been prepared to detect Hg 2+ in aqueous buffer solution, which demonstrates high selectivity for sensing Hg2+ with about 383-fold enhancement in fluorescence emission intensity and micromolar sensitivity.

102 citations

Journal ArticleDOI
TL;DR: In this article, a point defect model for the passive film was proposed to reproduce the experimental impedance at frequencies from 10 kHz to 1 mHz, at pH values from 9 to 11, and at applied voltages of 0.l and 0.5 V/sce.

102 citations

Journal ArticleDOI
TL;DR: A highly sensitive technique for the measurement of atmospheric HONO and HNO 3 is reported in this paper, which is based on aqueous scrubbing using two coil samplers, followed by conversion of HNO3 to nitrite, derivatization of nitrite to a highly light-absorbing azo dye with sulfanilamide (SA) and N -(1-naphthyl) ethylenediamine (NED), and high performance liquid chromatography (HPLC) analysis.

102 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202317
202232
202185
2020151
2019220
2018245