Topic
Buffer solution
About: Buffer solution is a research topic. Over the lifetime, 6948 publications have been published within this topic receiving 112440 citations. The topic is also known as: pH buffer & buffer.
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TL;DR: The difference between experimental and theoretical pH profiles is due to the inconsistency of the generally accepted model of the "unstirred layer", assuming the existence of a strict boundary between the regions of "pure diffusion" and "ideal stirring".
57 citations
TL;DR: In this article, hydrophilic copolymers of methacrylic acid (MAA) and methyl methacrylate (MMA) were prepared by suspension polymerization in an aqueous medium using 2,2'-azobisisobutyronitrile as the initiator and hydroxyapatite as the suspending agent.
57 citations
TL;DR: In this paper, the effects of a montmorillonite (M), a non-crystalline aluminium hydroxide (AL) and an Al(OH)2-montmorillonites complex (AM) on the adsorption, residual activity and kinetic properties of invertase were determined.
Abstract: The effects of a montmorillonite (M), a non-crystalline aluminium hydroxide (AL) and an Al(OH)2-montmorillonite complex (a montmorillonite partially coated with OH-Al ions; AM) on the adsorption, residual activity and kinetic properties of invertase were determined. In the range of pH 3.0–9.0 the ability of the clay minerals to adsorb protein molecules was in the order M > AM > AL and was influenced by the nature of the buffer solution. Adsorption resulted in a significant reduction of invertase activity (more than 85% at pH 4.65). the extent of which depended on clay mineral (AL > AM > M) and on pH. The free and immobilized enzyme showed maximum activity in the range of pH 4.5–5.5 and both states obeyed Michaelis-Menten kinetics. The Vmax, and Km values indicated a partial non-competitive inhibition of the clay minerals. Invertase adsorbed on AM and AL was more stable to thermal deactivation than was free enzyme, whereas invertase held on M was much less stable. In contrast, invertase adsorbed on M was more stable to the action of protease than free enzyme or that adsorbed on AM or AL.
57 citations
TL;DR: In this article, the diblock poly(N-isopropylacrylamide)-block-poly(methacrylic acid) (PNM) polymeric chains are grafted onto the membrane pore surfaces by using the surface initiated atom transfer radical polymerization (SI-ATRP) method.
57 citations
TL;DR: In this article, a stabilizing matrix able to generate ettringite from calcium sulphoaluminate in the presence of CaSO4·2H2O and Ca(OH)2 has been studied.
57 citations