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Showing papers on "Bufo melanostictus published in 2010"


Journal ArticleDOI
TL;DR: It is indicated that amphibian migration should be taken into account when roadside ditches are being planned and civil engineering should consider the needs of the amphibians’ living space and thereby reduce its impact on the amphibian’ original habitat, so that human beings live in harmony with nature.

18 citations


Journal Article
TL;DR: The skin secretion of Bufo melanostictus had no antibacterial activity when it was digested with a proteinase trypsin, and the result indicated that the antibacterial component was a protein.
Abstract: The skin secretion of Bufo melanostictus was collected and purified by separate proteins on a Sephadex G-25 columnAntimicrobial activity was determined by miniaturized methodsAntibacterial activities against the Gram positive bacteria Staphylococcus aureus and Bacillus subtilis,and the gram negative bacteria Aeromonas hydrophial were obserbedWeak antibacterial activity for the gram negative bacteria Valginolyticus,Vparahaemolyticus,Vfluvialis and Escherichia coli were also observedThe skin secretion of Bufo melanostictus had no antibacterial activity when it was digested with a proteinase trypsinThe result indicated that the antibacterial component was a proteinThe peptides cultured at various water temperature and pH showed antimicrobial stability against the temperature and pHIt was no hemolysis for fish red blood cells on the low concentration

1 citations