Showing papers on "Bufo melanostictus published in 2015"
TL;DR: The findings show that berberine possesses a skin-darkening potential and could be used as a safe melanogenic agent for the treatment of hypopigmentation disorders or vitiligo.
10 citations
TL;DR: Bufo melanostictus and Java are recorded as new host and locality for M. japonica, respectively, and Meteterakis wonosoboensis n.
Abstract: During a survey on the parasites of amphibians of Indonesia, toads (30 Bufo melanostictus) and 246 frogs (213 Fejervarya cancrivora, 11 F. limnocharis, 22 Rana macrodon from West Java and 68 F. cancrivora from Central Java) were examined for parasitic nematodes. Three species of nematodes were found and described, i.e. Meteterakis wonosoboensis n. sp. from Fejervaria cancrivora; Meteterakis japonica from Bufo melanostictus, F. cancrivora and F. limnocharis; and Chabaudus sp. from F. cancrivora, F. limnocharis and Rana macrodon. Meteterakis wonosoboensis n. sp. is distinguished from other species of the genus by the length and shape of spicules, the number of caudal papillae, the presence of gubernaculum in male and the presence of vulval flap in female. Bufo melanostictus and Java are recorded as new host and locality for M. japonica, respectively.
4 citations
Patent•
11 Nov 2015
TL;DR: In this article, a processing method for toad venom includes the steps that step 1, according to toad selection, high-quality adult bufo gargarizans or bufo melanostictus, the weight is larger than 50 g is selected; step 2, according cleaning, a bleaching powder water solution is used for soaking the toads; step 3, according smell removing, a mixed solution is prepared to soak the toad; step 4, according serous fluid collection, a method with no side effects or small side effects is used to stimulate the snakes to
Abstract: A processing method for toad venom includes the steps that step1, according to toad selection, high-quality adult bufo gargarizans or bufo melanostictus, the weight is larger than 50 g is selected; step2, according cleaning, a bleaching powder water solution is used for soaking the toads; step3, according to smell removing, a mixed solution is prepared to soak the toads; step4, according to serous fluid collection, a method with no side effects or small side effects is used for stimulating the toads to secrete serous fluid; step5, according to impurity filtering, red copper screens of 80 meshes and 120 meshes are used in sequence to filter away fine impurities of the toad serous fluid; step6, according to pre-drying, the liquid level is limited to be smaller than or equal to 3 cm, and then the toads stand in a dark place, so that block mass venom is obtained; step7, according to coloring, a baking light is used for baking the surface of the block mass venom; step8, according to drying, drying in the shade is performed for 10-15 days, and then a low-temperature microwave vacuum drier is used for obtaining cake venom, wherein the water content of the cake venom is 7-12%; step9, according to aging, vacuum packaging is performed on the cake venom, the cake venom is kept in the dark place, and therefore the toad venom finished product is obtained. Through the nine steps, the obtained toad venom is good in quality and color and luster, the collected toads are high in survival rate and recover rapidly, and the yield of the toad venom can be increased.
4 citations
Journal Article•
TL;DR: The anti-proliferative effects of Bufo melanostictus Schneider extracts on gastric cancer MGC803 cells were enhanced with increasing concentration and action time and the number of adherent cells decreased.
Abstract: To extract, isolate and identify active constituents in Bufo melanostictus Schneider, and to study their anti-proliferative and apoptosis-inducing effects on human gastric cancer MGC803 cells. Chemical constituents were isolated and purified by repeated silica gel column chromatography, ODS reverse phase column chromatography, preparative liquid chromatography and recrystallization, and structurally elucidated based on physicochemical properties & spectral data of compounds and reference literatures. Anti-proliferative effects of different concentrations of Bufo melanostictus Schneider extracts on gastric cancer MGC803 cells were detected by MTT, and their morphological changing effects on these cells were observed by microscopy. Four compounds were isolated from the skin of Bufo melanostictus Schneider. The anti-proliferative effects of Bufo melanostictus Schneider extracts on gastric cancer MGC803 cells were enhanced with increasing concentration and action time. 72 h after action of test solution in the high-dose group, proliferation inhibition rate of MGC-803 cells reached 77.5%. Under the microscope, firmly adherent MGC-803 cells with intact membranes were observed in the control group; cells were bright and clear, had good refraction, and were fully spread; uniform cytoplasmic distribution was observed as well. In various treatment groups, cell density became gradually sparse with increasing drug concentration, and cell surfaces were wrinkled. In the high-dose group, most cells were disrupted; cell morphology was not intact; cell refraction decreased; most cells were detached; chromatins were condensed, compacted and split into blocks; and the number of adherent cells decreased. Active constituents in Bufo melanostictus Schneider can induce apoptosis of gastric cancer MGC803 cells.
Journal Article•
TL;DR: Electrolytes level was high during aestivation and hibernation but level of Serum cholesterol, blood glucose and blood urea was low, During breeding and post breeding season electrolyteslevel was low but that of serum cholesterol,Blood urea and blood glucose was high.
Abstract: Serum electrolyte blood glucose, blood urea and serum cholesterol are estimated in four phases of annual biological cycle of Rana tigrina and Bufo melanostictus. Electrolytes level was high during aestivation and hibernation but level of Serum cholesterol, blood glucose and blood urea was low, During breeding and post breeding season electrolytes level was low but that of serum cholesterol, blood urea and blood glucose was high.