Topic
Calibration curve
About: Calibration curve is a research topic. Over the lifetime, 6552 publications have been published within this topic receiving 95128 citations.
Papers published on a yearly basis
Papers
More filters
TL;DR: An optical chemical sensor based on immobilization of 2-bromo-2-pyridylazo)-5-(diethylamino)phenol (Br-PADAP) in Nafion membrane is described and the results were compared with those obtained using atomic absorption spectrometry.
42 citations
TL;DR: An HPLC method that uses triple quadrupole mass detection has been validated with the objective both to improve the signal-to-noise (S/N) ratio and to achieve the maximum specificity for the analyte in those urine samples that are richer in possible inteferents.
Abstract: 1-Hydroxypyrene is a metabolite of pyrene, a member of the class of polycyclic aromatic hydrocarbons (PAHs) whose toxic properties in some cases include carcinogenicity. The determination of 1-hydroxypyrene in human urine is used as a biological indicator for exposure to PAHs, which is related to the combustion of organic materials, like smoking, living in urban environments, and eating grilled or smoked food. The determination of 1-hydroxypyrene by high-performance liquid chromatography (HPLC) with fluorescence detection has very good sensitivity but it is not highly specific: this can reduce accuracy in the quantitative determination of low levels of analyte in a complex matrix like urine. An HPLC method that uses triple quadrupole mass detection has been validated with the objective both to improve the signal-to-noise (S/N) ratio and to achieve the maximum specificity for the analyte in those urine samples that are richer in possible inteferents. The calibration range for 1-hydroxypyrene is from 0.005-0.1 microg/L in the urine of non-smoking healthy volunteers. After solid-phase extraction, samples were analyzed by HPLC/tandem mass spectrometry (MS/MS) in the multiple reaction monitoring (MRM) mode. In order to obtain reliable results quantitative analysis must be performed by means of the internal standard method (we used deuterium-labelled 1-hydroxypyrene): the method accuracy is not less than 85%. The S/N ratio at a concentration of 0.1 microg/L is about 10, and therefore this can be considered the lowest limit of quantitation. The method performance does not change if urine samples are measured using a calibration curve prepared in methanol, thus reducing the time of analysis and costs.
42 citations
TL;DR: The results demonstrate that when an analyte contributes to IS signal, linearity and accuracy can be affected with low IS concentration, and measures to reduce their impact are proposed.
42 citations
TL;DR: The optimization and validation of a quick and simple method for the simultaneous determination of total content and available fractions of As, Cr, Cu, Ni, Pb and Tl in sediments by ET AAS is described, which has been proved to be useful for environmental research.
42 citations
TL;DR: A new type of polarographic electrode (Clark) was found to provide a means of accurately and rapidly determining the O2 tension of whole blood at all levels of Po2.
Abstract: A new type of polarographic electrode (Clark) was found to provide a means of accurately and rapidly determining the O2 tension of whole blood at all levels of Po2. The electrode response is independent of ph, CO2 tension and hematocrit. Temperature and pressure changes produce predictable linear variations in electrode response for any given oxygen tension. There were found to be occasional marked variations in calibration curves for unknown reasons. Thus, accuracy can only be assured by developing a calibration curve for each subject's blood. A simple system for calibration of the electrode in whole blood has been described. Submitted on June 14, 1957
42 citations