Topic
Calibration curve
About: Calibration curve is a research topic. Over the lifetime, 6552 publications have been published within this topic receiving 95128 citations.
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TL;DR: A simple, rapid and sensitive high-performance liquid chromatographic method for the simultaneous determination of captopril and hydrochlorothiazide in human plasma samples was developed and suitably validated and successfully applied.
123 citations
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TL;DR: In this paper, a valveless flow-injection analysis system incorporating a series of functional modules, provided by a set of flat elements that are stacked vertically to form a series internal flow streams, has been developed.
Abstract: A valveless flow-injection-analysis system incorporating a series of functional modules, provided by a set of flat elements that are stacked vertically to form a series of internal flow streams, has been developed. A dye is used for sample introduction and injection and the system is coupled to an optical detector. The samples undergo a minimal amount of dilution, retaining 96–97% of their original concentration. Good run-to-run reproducibility is achieved, as indicated by measured standard deviations of less than 1%. A calibration curve has been generated and produces a linear fit to the data. The use of micromachining techniques to produce the set of flat elements and therefore to manufacture large quantities of identical flow-injection systems is discussed.
122 citations
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TL;DR: An electro membrane extraction (EME) methodology was utilized to study the isolation of some environmentally important pollutants from aquatic media based upon the electrokinetic migration process to show a rather high degree of selectivity towards pentachlorophenol (PCP).
120 citations
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TL;DR: In this article, a wide spectral range time resolved laser-induced breakdown spectroscopy has been investigated to evaluate its potential for simultaneous determination of major, minor and trace concentrations in water.
119 citations
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TL;DR: A modification of competitive PCR which is based on standardization of interpolation from a calibration curve which enables accurate analysis of multiple samples by comparison with a single set of standard samples containing known amounts of the wild-type template of interest.
Abstract: PCR is an exquisitely sensitive method for detection of minute amounts of target DNA. The exponential nature of DNA amplification, however, is prone to burden the experimental data with significant standard error due to the tube-to-tube inherent variations in amplification efficiency. Therefore the reliable quantitation of DNA or RNA templates by PCR necessitates introduction of an internal standard. Typically, in a series of replicates of analyzed sample the defined range of such standards is coamplified together with constant unknown amount of wildtype template (1, 2) or alternatively both templates are subjected to endpoint dilution (3). Obviously, each of the methods is based on multiple PCR reactions which are to be performed simultaneously with each of the analyzed samples. Such procedure, nevertheless, may become cumbersome when large numbers of samples are to be analyzed. We describe a modification of competitive PCR which is based on standardization of interpolation from a calibration curve. This approach enables accurate analysis of multiple samples by comparison with a single set of standard samples containing known amounts of the wild-type template of interest. Effective compensation for intra-assay variability is achieved using a constant amount of internal standard to spike all samples being analyzed as well as the samples used as standards. The comprehensive equation — logiN^/N^) = \og(N01/Ng^) + nlogieff^effi) defines the yield of the two products in any competitive PCR in terms of the ratio of the two original templates and their relative efficiencies of amplification, and simplifies when the products of both the endogenous wild-type template and the exogenous internal standard are generated with the same efficiency. Provided that this requirement (effi = effd is
118 citations