Topic
Candida rugosa
About: Candida rugosa is a research topic. Over the lifetime, 916 publications have been published within this topic receiving 23195 citations.
Papers published on a yearly basis
Papers
More filters
870 citations
TL;DR: The structure of the Candida rugosa lipase determined at 2.06-A resolution reveals a conformation with a solvent-accessible active site and provides a new image of the substrate binding region and active site access, which is different from that inferred from theructure of the "closed" form of the G. candidum lipase.
535 citations
TL;DR: In this paper, the stability and enzymatic activity of Candida rugosa Lipase (E.C.1.3) immobilized on γ-Fe2O3 magnetic nanoparticles were reported.
Abstract: We report the stability and enzymatic activity of Candida rugosa Lipase (E.C.3.1.1.3) immobilized on γ-Fe2O3 magnetic nanoparticles. The immobilization strategies were either reacting the enzyme amine group with a nanoparticle surface acetyl, or amine groups. In the former, the enzyme was attached through a CN bond, while in the latter it was connected using glutaraldehyde. AFM images show an average particle size of 20 ± 10 nm after deconvolution. The enzymatic activity of the immobilized lipase was determined by following the ester cleavage of p-nitrophenol butyrate. The covalently immobilized enzyme was stabile and reactive over 30 days.
531 citations
01 Jan 2003
453 citations
TL;DR: A method for immobilization of Candida rugosa lipase to two types of chitosan beads by activating the hydroxyl groups of ch itosan using carbodiimide coupling agent has been successfully developed.
371 citations