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Showing papers on "Canine Mastocytoma published in 2009"


Journal ArticleDOI
TL;DR: The C2 cell line was radiosensitive, and a fraction of cells died via apoptosis in a dose-dependent manner, and in response to radiation, C2 cells did not upregulate VEGF production or VEGFR.
Abstract: Objective—To establish the radiosensitivity and effect of irradiation on vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) expression in the canine mastocytoma cell line C2. Sample Population—Canine mastocytoma cell line C2. Procedures—C2 cells were irradiated with single doses of 2, 4, 6, and 8 Gy. The 3-(4, 5-di-methyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide assay and proliferation assays with (methyl-hydrogen 3) thymidine were used for radiosensitivity experiments. Expression of VEGFR was determined via flow cytometry and apoptotic rate via annexin assay. Human and canine VEGF ELISA kits were evaluated in crossover assay experiments, and the canine kit was used thereafter. Results—C2 cells secreted VEGF constitutively. Radiation did not induce a significant increase in VEGF secretion, regardless of radiation dose. Consistently, radiation did not up-regulate VEGFR. Cell survival rates decreased in a dose-dependent manner. The apoptotic cell fraction had a dose-dependent incre...

10 citations


Journal ArticleDOI
TL;DR: The effect of TRAIL on the canine C2 mastocytoma cell line suggests that this cytokine might be suitable for treatment of mast cell tumors in dogs.

7 citations


01 Jan 2009
TL;DR: Two patients brought in to the Emergency Clinic for consultation were diagnosed with canine mastocytoma, which started as a single nodule and combined therapy of vinblastine and prednisolon has proven poor efficiency.
Abstract: Mast cells are well known for their neoplastic transformation in solitary and multiple cutaneous mast cell tumours, as well as visceral and systemic mastocytosis. In this paper we are presenting 2 cases brought in our Emergency Clinic for consultation, which were diagnosed with canine mastocytoma. In both of the patients, the tumor started as a single nodule. They were diagnosed with grade 3 mastocytoma using cytological and histopathological exam. Combined therapy of vinblastine and prednisolon has proven poor efficiency. Survival times, after the diagnosis, were 35 days (patient 1) and 22 days respectively (patient 2).

3 citations


Journal ArticleDOI
TL;DR: It is proposed that the identification of the precise signaling defect in C2 cells will yield useful information regarding the pathway leading to mast cell exocytosis and facilitate the restoration of the complete signaling cascade through complementation of the missing/defective signal transducer.

2 citations