Showing papers on "Canine Mastocytoma published in 2018"
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TL;DR: The JAK2/STAT5 pathway is a novel potential target of therapy in canine mastocytoma and synergistic anti-neoplastic effects were obtained by combining pimozide with KIT-targeting drugs in NI-1 cells.
Abstract: Background
Mastocytoma are frequently diagnosed cutaneous neoplasms in dogs. In non-resectable mastocytoma patients, novel targeted drugs are often applied. The transcription factor STAT5 has been implicated in the survival of human neoplastic mast cells (MC). Our study evaluated the JAK2/STAT5 pathway as a novel target in canine mastocytoma.
Materials and Methods
We employed inhibitors of JAK2 (R763, TG101348, AZD1480, ruxolitinib) and STAT5 (pimozide, piceatannol) and evaluated their effects on 2 mastocytoma cell lines, C2 and NI-1.
Results
Activated JAK2 and STAT5 were detected in both cell lines. The drugs applied were found to inhibit proliferation and survival in these cells with the following rank-order of potency: R763 > TG101348 > AZD1480 > pimozide > ruxolitinib > piceatannol. Moreover, synergistic anti-neoplastic effects were obtained by combining pimozide with KIT-targeting drugs (toceranib, masitinib, nilotinib, midostaurin) in NI-1 cells.
Conclusion
The JAK2/STAT5 pathway is a novel potential target of therapy in canine mastocytoma.
16 citations