Topic
Canine Mastocytoma
About: Canine Mastocytoma is a research topic. Over the lifetime, 59 publications have been published within this topic receiving 1417 citations.
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TL;DR: The C2 cell line was radiosensitive, and a fraction of cells died via apoptosis in a dose-dependent manner, and in response to radiation, C2 cells did not upregulate VEGF production or VEGFR.
Abstract: Objective—To establish the radiosensitivity and effect of irradiation on vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) expression in the canine mastocytoma cell line C2. Sample Population—Canine mastocytoma cell line C2. Procedures—C2 cells were irradiated with single doses of 2, 4, 6, and 8 Gy. The 3-(4, 5-di-methyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide assay and proliferation assays with (methyl-hydrogen 3) thymidine were used for radiosensitivity experiments. Expression of VEGFR was determined via flow cytometry and apoptotic rate via annexin assay. Human and canine VEGF ELISA kits were evaluated in crossover assay experiments, and the canine kit was used thereafter. Results—C2 cells secreted VEGF constitutively. Radiation did not induce a significant increase in VEGF secretion, regardless of radiation dose. Consistently, radiation did not up-regulate VEGFR. Cell survival rates decreased in a dose-dependent manner. The apoptotic cell fraction had a dose-dependent incre...
10 citations
01 Jan 2019
TL;DR: The clinical and hematological changes in a patient with canine mastocytoma when submitted to chemotherapy and electrochemotherapy is reported.
Abstract: Mastocytoma is a round cell neoplasm often found in dogs, accounting for 7% to 21% of cutaneous neoplasms. This study aimed to report the clinical and hematological changes in a patient with canine mastocytoma when submitted to chemotherapy and electrochemotherapy. It was attended at the Metropolitan Veterinary Hospital (HVM) of Caucaia Ceará, a bitch without a defined breed presenting a cutaneous nodule in the right knee. Initial examinations were requested, where the cytologic diagnosis was suggestive of mastocytoma, in which the absence of metastasis was evidenced by the imaging tests. The female was referred for evaluation with an oncologist, who instituted as treatment the surgical removal of the nodule with the use of electrochemotherapy on the lateral and deep margins, still requiring a cutaneous graft. Histopathological analysis indicated grade II mastocytoma. After 32 days of the surgical procedure, the patient underwent the first chemotherapy session and with one week the patient follow-up examinations showed marked hematological alterations, also showing tiredness, dyspnea and hyporexia, resulting from pneumonia and the presence of free pleural fluid, whose cytological analysis revealed a pyogranulomatous inflammatory process. In the case of dogs with canine mastocytoma submitted to Revista Brasileira de Higiene e Sanidade Animal Brazilian Journal of Hygiene and Animal Sanity ISSN: 1981-2965
10 citations
TL;DR: The results suggest that stimulation using MF and Co0.2Mn0.8Fe2O4 nanoparticles is involved in mechanisms associated with controlling cell proliferative potential signaling events, and shows that nanoparticles and MF elevate the temperature in vitro in tumor cells, thereby increasing the expression of ROS as well as heat shock proteins.
Abstract: Cobalt manganese ferrite nanoparticles have application potential in the biomedical field, however there is limited information concerning the biological response. The aim of this work was to investigate the cytotoxic potential of cobalt-manganese ferrite nanoparticles in canine mastocytoma tumor cells (C2) and adipose-derived mesenchymal stromal stem cells (ASCs) cultured under a static magnetic field (MF). In this study, we investigated the viability and proliferation rate of ASC and C2 cells cultured with Co0.2Mn0.8Fe2O4 nanoparticles under 0.5T MF. We observed cells morphology and measured intracellular ROS generation. Thermal observations were used to characterize the thermotrophic cell behavior in different condition and RNA level of heat shock proteins and apoptotic genes was measured. Nanoparticles reduced cell viability, caused cell damage, i.e., through the formation of reactive oxygen species (ROS) and increased transcriptional level of apoptotic genes (Bcl-2, Bax, p53, p21). In addition, we have found that C2 mastocytoma cells cultured with metal oxide nanoparticles under MF exhibited unexpected biological responses, including thermotolerance and apoptotic response induced by the expression of heat shock proteins and ROS produced under a MF. Our results suggest that stimulation using MF and Co0.2Mn0.8Fe2O4 nanoparticles is involved in mechanisms associated with controlling cell proliferative potential signaling events. We can state that significant differences between normal and cancer cells in response to nanoparticles and MF are apparent. Our results show that nanoparticles and MF elevate the temperature in vitro in tumor cells, thereby increasing the expression of ROS as well as heat shock proteins.
9 citations
TL;DR: It is suggested that the relatively greater affinity of the murine sulfomucopolysaccharide for amino-containing compounds explains the high concentration of nitrogen in crude extracts of the Murine Sulfomucpolysaccharides.
8 citations
TL;DR: The cloning of cDNAs encoding two canine IL-13 receptor α chains, both of which were found to contain the highly conserved motifs, such as cysteine and tryptophan residues in their N-terminal portion and the WSXWS at C-terminus are reported.
7 citations