Topic
Catechol-O-methyl transferase
About: Catechol-O-methyl transferase is a research topic. Over the lifetime, 1646 publications have been published within this topic receiving 87360 citations.
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TL;DR: Results indicate that the activities of MAO‐A and PST in the monolayers approach those found in the gray matter, and additional studies with both tissues suggest that the PST of both tissues is the thermostable form of the enzyme.
Abstract:
The activities of monoamine oxidase (MAO), catechol-O-methyltransferase (COMT), phenol sulfotransferase (PST), alkaline phosphatase (AP), γ-glutamyl transpeptidase (GT), and angiotensin converting enzyme (ACE) were quantitated in primary cultures of bovine brain microvessel endothelial cell monolayers and cerebral gray matter. Significant MAO-A and -B, cytosolic and membrane-bound COMT, PST, AP, GT, and ACE activities are demonstrated in bovine gray matter. By comparison, enzyme activities of the monolayers vary with the age of the monolayer and are generally higher in complete monolayers. Relative to gray matter enzyme activities, the monolayers are enriched with AP, GT, and ACE, enzymes considered to be markers for brain endothelium. Results also indicate that the activities of MAO-A and PST in the monolayers approach those found in the gray matter. Conversely, cytosolic COMT and MAO-B activities in the monolayers are negligible and much lower, respectively, compared to activities in gray matter. Additional studies with both tissues suggest that the PST of both tissues is the thermostable form of the enzyme.
72 citations
Bial1
TL;DR: The results reported here show that tolcapone is particularly potent in inhibiting MB-COMT from liver and brain under in vivo experimental conditions, though it does not discriminate between MB- and S- COMT under in vitro experimental conditions when using the same amount of enzyme in the assay.
72 citations
TL;DR: It is concluded that tolcapone can either slightly improve or impair the memory functions depending on task specific elements and performance factors.
72 citations
TL;DR: It is found that gender may be an important variable in consideration of the impact of COMT on cognition, and when gender is taken into consideration, any negative impact of comT genotype may extend to cognitive domains other than those associated with prefrontal regions.
72 citations
TL;DR: It is suggested that the COMT val(158)met polymorphism may affect age-related differences in gray and white matter density in females.
72 citations