Topic
Cell growth
About: Cell growth is a research topic. Over the lifetime, 104237 publications have been published within this topic receiving 3751303 citations. The topic is also known as: GO:0016049 & cellular growth.
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TL;DR: The patterns of mRNA and protein expression of 7 protein kinase C (PKC) isozymes in NIH 3T3 cells are determined and high expression of PKC-epsilon contributes to neoplastic transformation.
546 citations
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TL;DR: It is demonstrated that purified E. coli-derived recombinant human TNF inhibits encephalomyocarditis virus replication in "aged" human fibroblasts, suggesting that IFN-beta 2 has biological functions distinct from the other interferons.
546 citations
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TL;DR: Ascorbic acid enhanced the antilymphoma effect of As2O3 in vivo without additional toxicity, and may provide a novel therapy for lymphoma.
546 citations
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TL;DR: Using strains where CLN1 was expressed conditionally, the essential function of ClN proteins was found to be limited to the G1 phase, consistent with the hypothesis that Cln proteins activate the Cdc28 protein kinase, shown to be essential for the G 1 to S phase transition in S. cerevisiae.
546 citations
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TL;DR: In vitro kinase activity assays of PKC immunoprecipitates from the conjugates of T cells and APCs showed a selective increase in the activity ofPKC-Θ, indicating that the translocated enzyme is active.
Abstract: Every cell contains many families of protein kinases, and may express several structurally related yet genetically distinct kinases of each family. The activity of the serine/threonine protein kinase C (PKC) enzymes has long been implicated in T-cell activation, but it is not known which members of the PKC family regulate the T-cell response to foreign antigens. The activation of T cells by antigen-presenting cells (APCs) is spatially restricted to their site of contact, where receptors on the T cells engage their counter-receptors on the APCs. We used this localized engagement to identify, at the single-cell level, intracellular proteins involved in the activation process. By digital immunofluorescence microscopy, we localized six isoforms of PKC in antigen-specific T-cell clones activated by APCs. Surprisingly, only PKC-theta translocated to the site of cell contact. Accordingly, in vitro kinase activity assays of PKC immunoprecipitates from the conjugates of T cells and APCs showed a selective increase in the activity of PKC-theta, indicating that the translocated enzyme is active. Several modes of partial T-cell activation that failed to cause PKC-theta translocation also failed to cause T-cell proliferation, further suggesting the involvement of PKC-theta in T-cell activation.
545 citations