Showing papers on "Cellular compartment published in 1972"

Pancreas acinar cell differentiation. VII. Autoradiographic demonstration of intracellular radioactivity with with S-adenosyl-L-(C 3 H 3 ) methionine.

Abstract: After rat pancreas anlagen were grown in an organ culture medium for 9 days and further incubated for 15, 30 or 60 minutes in media to which C3H3-Sadenosyl-L-methionine (C3H3-SAM) was added, autoradiography demonstrated radioactivity in the nucleolus, nucleus and cytoplasm of the acinar cell. Under similar circumstances, except that the anlagen were grown in a methionine-deficient (CD-MD) medium prior to incubation with C3H3-SAM, much greater concentrations of radioactivity were present in the nucleolus, nucleus and cytoplasm of the acinar cell than in the respective compartments of anlagen grown in control medium. The nucleolus showed the highest concentration of radioactivity in anlagen grown in either control or methionine-deficient medium. The nucleolus of the acinar cell of anlagen grown in CD-MD medium appeared to be the most undermethylated cellular compartment and was larger than the nucleolus of the acinar cell grown in the control CD medium, so it is possible that a deficient methylation of ribosomal RNA precursors led to a piling up of RNA in the nucleolus and a decrease in the flow of ribosomal precursors to the cytoplasm. Other substance might have been undermethylated and thereby have inhibited differentiation.

Intracellular Clinical Chemistry

Abstract: The plasma compartment, on which almost all routine analyses in clinical chemistry are performed, occupies only a small part of the body, most of which consists of cells. For most components the intracellular and extracellular compartments have a different composition in health, and change differently in disease. The readily available cells are erythrocytes, and although these can be used for many purposes, including vitamin studies, they should not be used for studies of water and electrolytes. Muscle cells and leukocytes are the other available cells: they present different problems of isolation and methodology, but valid work has been done on their use for studies of water, electrolytes, and pH in health and disease. A recently developing study is the identification of inborn errors of metabolism by analysis of leukocytes and other cells. All cellular studies offer problems in that changes in one type of cell cannot necessarily be applied to changes in the cellular compartment of the body as a whole.