Chemical binding

About: Chemical binding is a research topic. Over the lifetime, 1822 publications have been published within this topic receiving 52516 citations.

Surface plasmon resonance immunosensor for detection ofLegionella pneumophila

Abstract: An immunosensor based on surface plasmon resonance (SPR) onto a protein G layer by self-assembly technique was developed for detection ofLegionella pneumophila. The protein G layer by self-assembly technique was fabricated on a gold (Au) surface by adsorbing the 11-mercaptoundecanoic acid (MUA) and an activation process for the chemical binding of the free amine (-NH2) of protein G and 11-(MUA) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) in series. The formation of the protein G layer by self-assembly technique on the Au substrate and the binding of the antibody and antigen in series were confirmed by SPR spectroscopy. The surface topographies of the fabricated thin films on an Au substrate were also analyzed by using an atomic force microscope (AFM). Consequently, an immunosensor for the detection ofL. pneumophila using SPR was developed with a detection limit of up to 102 CFU per mL.

Binding of Leishmania spp with gold nanoparticles supported polyethylene glycol and its application for the sensitive detection of infectious photogenes in human plasma samples: A novel biosensor.

Abstract: The management of pathogen detection using a rapid and cost-effective method presents a major challenge to the biological safety of the world. The field of pathogen detection is nascent and therefore, faces a dynamic set of challenges as the field evolves. Visceral leishmaniasis (VL), or kala-azar is the most severe form of leishmaniasis. Delay to the accurate diagnosis and treatment is likely to lead to fatality. The reliable, fast and sensitive detection is closely linked to safe and effective treatment of Leishmania spp. Despite several routine and old method for sensitive and specificity detection of Leishmania spp, there is highly demand for developing modern and powerfully system. In this study a novel ultra-sensitive DNA-based biosensor was prepared for detection of Leishmania spp. For the first time, the specific and thiolated sequences of the Leishmania spp genome (5'-SH-[CH2 ]6 ATCTCGTAAGCAGATCGCTGTGTCAC-3') were recognized by electrochemical methods. Also, selectivity of the proposed bioassay was examined by three sequences that were mismatched in 1, 2, and 3 nucleotides. The linear range (10-6 to 10-21 M) and limit of detection (LLOQ = 1 ZM) obtained are remarkable in this study. Also, simple and cost-effective construction of genosensors was another advantage of the proposal DNA-based assay. The experimental results promise a fast and simple method in detection of kala-azar patients with huge potential of the nanocomposite-based probe for development of ideal biosensors.

Antimicrobial activity of silk treated with Aloe-Vera

Abstract: Natural Silk in its native form is made up of the filament fibroin, coated with sericin - gummy matter which is antimicrobial in nature. However, sericin is removed during the pretreatment process. Silk being a natural and hygroscopic, fiber gets attacked by microbes easily. Hence antimicrobial treatment can add value to the wear and care of silk textiles. Synthetic antimicrobial agents may be toxic and even carcinogenic and hence the natural and eco-friendly antimicrobial agents are good substitutes for imparting the desired properties. In the present paper, ready for dyeing (RDF) silk fabric was treated with Aloe-Vera using 1,2,3,4-butanetetracarboxylic acid (BTCA) as crosslinking agent and sodium hypophosphite (SHP) as catalyst. The treated fabric with a concentration of 15 % of Aloe-Vera showed excellent antimicrobial properties. Since BTCA was used as a crosslinking agent, crease recovery angle, improved with minimal loss in breaking and tearing strength. The mechanism of treatment of Aloe-Vera is found to be chemical binding with silk and not simply of coating or impregnation. FTIR studies showed that the carboxyl side groups and short chain amino acids side groups act as sites for BTCA crosslinking interalia chemical binding of Aloe-Vera. SEM studies revealed that no coating or tangible impregnation on the surface of the fiber is visible substantiating the chemical binding phenomenon. This is further substantiated by the durability of the finish to dry cleaning of treated silk. Since Aloe-Vera is a natural product and BTCA is an eco-friendly resin, the treatment of silk with Aloe-Vera is eco-friendly in nature.

Nitrogen-doped hollow porous carbon nanospheres coated with MnO2 nanosheets as excellent sulfur hosts for Li–S batteries

Abstract: In this work, nitrogen-doped hollow porous carbon nanospheres coated with MnO2 nanosheets (NHPC@MnO2) were prepared as a novel sulfur host for the cathode of lithium-sulfur battery. N-doping of carbon and deposition of the inherently polar MnO2 promote chemical binding of the host with sulfur and its reduction products, known as polysulfides. Meanwhile, proper N-doping can improve the electron conductivity of carbon, and the nanosheet structure may help to guarantee facile electron- and lithium-ion transport through MnO2. Attributed to these advantages, the NHPC@MnO2/S cathode with a high sulfur content (70 wt% and 2.6 mg cm-2) exhibited an excellent cycle stability: its capacity retention was 93% within 100 cycles at 0.5 C. It also displayed a good rate capability: discharge capacities being ∼1130 mAh g-1 at 0.2 C, ∼1000 mAh g-1 at 0.5 C, ∼820 mAh g-1 at 1 C, and ∼630 mAh g-1 at 2 C. Our work demonstrates the synergistic effect of MnO2 nanostructure and N-doped carbon nanospheres for enhanced performance of lithium-sulfur battery cathodes.