Topic
Chitin
About: Chitin is a research topic. Over the lifetime, 6590 publications have been published within this topic receiving 253993 citations.
Papers published on a yearly basis
Papers
More filters
TL;DR: CAP-1 inhibited precipitation of calcium carbonate in an in vitro anticalcification assay dose-dependently, and completely inhibited it at 3×10-7 M, indicating that this molecule was bifunctional and played an important role in formation of the exoskeleton.
Abstract: Organic matrices in calcified hard tissues have been considered to control calcification. A matrix peptide, designated CAP-1, was extracted and purified by anionexchange and reverse-phase high performance liquid chromatographies from the exoskeleton of the crayfish, Procambarus clarkii. The amino acid sequence of CAP-1 was determined by mass spectral and sequence analyses of the intact peptide and its enzymatically digested peptides. CAP-1 consisted of 78 amino acid residues, including a phosphoserine residue, and was rich in acidic amino acid residues. CAP-1 had a RebersRiddiford consensus sequence, which is conserved in cuticle proteins from many arthropods. CAP-1 inhibited precipitation of calcium carbonate in an in vitro anticalcification assay dose-dependently, and completelyinhibited it at 3×10-7 M. CAP-1 also showed chitinbinding ability, indicating that this molecule was bifunctional and played an important role in formation of the exoskeleton.
105 citations
TL;DR: Observations support the notion that the insolubilization of the glucan is caused by linkage to chitin, and the linkage of the two polymers significantly contributes to the mechanical strength of the cell wall.
Abstract: Pulse-chase experiments with [14C]glucose demonstrated that in the cell wall of wild-type Saccharomyces cerevisiae alkali-soluble (1-3)-beta-glucan serves as a precursor for alkali-insoluble (1-3)-beta-glucan. The following observations support the notion that the insolubilization of the glucan is caused by linkage to chitin: (i) degradation of chitin by chitinase completely dissolved the glucan, and (ii) disruption of the gene for chitin synthase 3 prevented the formation of alkali-insoluble glucan. These cells, unable to form a glucan-chitin complex, were highly vulnerable to hypo-osmotic shock indicating that the linkage of the two polymers significantly contributes to the mechanical strength of the cell wall. Conversion of alkali-soluble glucan into alkali-insoluble glucan occurred both early and late during budding and also in the ts-mutant cdc24-1 in the absence of bud formation.
105 citations
TL;DR: The chitin isolated from various natural sources is suitable for biotechnological applications (e.g. as supporting material for immobilizing enzymes) and its degree of acetylation was determined.
Abstract: Chitin was isolated from various natural sources including Cuban lobsters, Sanlucar prawns, Norway lobsters, Squills, Spanish crayfish, American crayfish and Fusarium oxysporum with a yield of 14–25% on a dry basis.
The physico-chemical properties of chitin from the different sources were studied by IR spectroscopy and scanning electron microscopy, and its degree of acetylation was determined. The chitin thus obtained is suitable for biotechnological applications (e.g. as supporting material for immobilizing enzymes).
105 citations
TL;DR: There is strong evidence that OnCht plays an important role in regulating chitin content of the PM and subsequently affecting the growth and development of the ECB larvae.
105 citations
TL;DR: The data suggest that chitin synthesis is critical to microfilarial sheath morphogenesis in this parasitic nematode.
105 citations