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Chitin
About: Chitin is a research topic. Over the lifetime, 6590 publications have been published within this topic receiving 253993 citations.
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TL;DR: The thermally-induced conversion of chitosonium acetate in film form into a water-insoluble chitin film was examined by thermal analysis (DMTA, TGA, DSC, and TMA) and by solid state 13C-NMR spectroscopy as mentioned in this paper.
Abstract: The water-soluble solids comprised of the ionic complex between chitosan and acetic acid, chitosonium acetate, are converted into chitin by heating. The thermally-induced conversion of a water-soluble chitosonium acetate in film form into a water-insoluble chitin film was examined by thermal analysis (DMTA, TGA, DSC, and TMA) and by solid state 13C-NMR spectroscopy. Results indicate that tan δ-transitions occur at increasingly high temperatures, and over progressively wider temperature ranges, as the transformation progresses. Likewise, the storage modulus, log E′, increases as the chitosonium acetate film undergoes “cure” and converts to chitin. Cure kinetic parameters are obtained using the model proposed by Provder et al. modified for glass transition temperature (Tg). The results suggest the existence of two sequential first order reactions, an initial and a late cure reaction, having activation energies of approximately 15 and 21 kcal/mol, respectively. © 1996 John Wiley & Sons, Inc.
87 citations
TL;DR: Chitinase activity was confirmed on non-denaturing one-dimensional and two-dimensional gels using a sandwich assay with glycol chitin as a substrate and two of the proteins had similarities with endochitinases as shown by their N-terminal amino acid sequences.
87 citations
TL;DR: It could not, however, be conclusively established whether protein and carbohydrate are covalently linked, because the chitinase is resistant to endo-beta-N-acetyl-glucosaminidase.
87 citations
TL;DR: It is demonstrated for the first time that a chitin-like material is present in A. aegypti eggs, ovaries and eggshells, and a Chitin synthesis inhibitor can be used to inhibit mosquito oogenesis, and chitIn synthesis inhibitors have potential for controlling mosquito populations.
87 citations
TL;DR: The results showed that Ganoderma lucidum chitin has similar characteristic of chit in from different fonts as well as the advantage of the biotechnological processes and the fact that the fungus may be used as a potential raw material for chitIn production were demonstrated.
Abstract: The chitin was isolated from the Ganoderma lucidum submerged cultures mycelium as potential source of chitin under biotechnological processes. The extraction of chitin was carried out through 5 different assays which involved mainly three phases: pulverization of the mushroom, deproteinization of the mycelia with NaOH solution, and a process of decolorization with potassium permanganate and oxalic acid. The chitin contents extracted from 9-day mycelia were 413, 339, 87, 78, and 144 mg/g(-1) (milligrams of chitin/grams of dry biomass) for A1, A2, A3, A4, and A5, respectively. Obtained chitin was characterized by X-Ray Diffraction (XRD), by Fourier transform infrared spectroscopy (FTIR), and by thermal analysis (TGA). The results showed that Ganoderma lucidum chitin has similar characteristic of chitin from different fonts. The advantage of the biotechnological processes and the fact that Ganoderma lucidum fungus may be used as a potential raw material for chitin production were demonstrated.
87 citations