Topic
Chitin
About: Chitin is a research topic. Over the lifetime, 6590 publications have been published within this topic receiving 253993 citations.
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TL;DR: An enzyme that catalyzes hydrolysis of acetamido groups of chitin derivatives was found in the supernatant fraction of Mucor rouxii and accounts for the formation of Chitosan in fungi.
Abstract: 1. An enzyme that catalyzes hydrolysis of acetamido groups of chitin derivatives was found in the supernatant fraction of Mucor rouxii. 2. Partially O-hydroxyethylated chitin (glycol chitin) was used as a substrate in the purification and characterization of this enzyme. A 140-fold purification was obtained by means of ammonium sulfate fractionation followed by chromatography on carboxymethylcellulose and DEAE-cellulose. 3. The enzyme releases about 30% of the acetyl groups of glycol chitin, giving a product with a decreased sensitivity to lysozyme. The enzyme also deacetylates chitin and N-acetylchitooligoses, whereas it is inactive toward bacterial cell wall peptidoglycan, N-acetylated heparin, a polymer of N-acetylgalactosamine, di-N-acetylchitobiose and monomeric N-acetylglucosamine derivatives. 4. This enzyme shows a pH optimum of 5.5. The Km value for glycol chitin is 0.87 g/l or 2.6 mM with respect to monosaccharide residues. 5. The occurrence of this enzyme accounts for the formation of chitosan in fungi.
203 citations
TL;DR: In this article, the effect of solvent composition and temperature on chitin structure was investigated with an optical microscope, from which 8 Wt% NaOH/4 Wt % urea concentrations were deduced as suitable and −20°C as the appropriate temperature.
202 citations
TL;DR: The effect of chitin, poly-β-(1→4)- N -acetyl-glucosamine, and chitosan, a polymer of glucosamine obtained by the deacetylation of ch itin, on growth and nutrient digestibility was studied in tilapia.
202 citations
TL;DR: In this article, the authors described the direct conversion of chitin into a nitrogen-containing (N-containing) furan derivative (3A5AF) for the first time under optimized conditions, the yield of 3A5F reached 75% with ca 50% chitIN conversion by using boric acid and alkaline chlorides as additives, and NMP as a solvent.
201 citations
TL;DR: Total Phenolic content of soybean leaves increased following chitosan and chitin oligomer treatments, showing a positive correlation between enzyme activity and total phenolic content.
201 citations