Showing papers on "Chitinase published in 1971"
TL;DR: Both glucanase and chitinase were extracted from ethylene-treated bean leaves and purified on hydroxyapatite and carboxymethyl Sephadex columns and had an isoelectric point near pH 11 and was specific for beta-1, 3-linkages.
Abstract: Glucanase (endo-beta-1, 3-glucan 3-glucanohydrolase, EC 3.2.1.6, laminarinase, callase) and chitinase (poly-beta-1, 4-[2-acetamido-2-deoxy] -d-glucoside glycanohydrolase, EC 3.2.1.14) were extracted from ethylene-treated bean (Phaseolus vulgaris L. cv. Red Kidney) leaves and purified on hydroxyapatite and carboxymethyl Sephadex columns. The glucanase prepared was homogeneous as judged by analytical centrifugation data, electrophoresis, and antibody-antigen reactions. On the basis of gel filtration, antibody-antigen reactions, and amino acid analysis, the molecular weight was estimated to be between 11,500 and 12,500. However, ultracentrifugation gave a higher estimate of 34,000. The glucanase had an isoelectric point near pH 11 and was specific for beta-1, 3-linkages. The chitinase was only partially purified as judged by electrophoretic behavior.
372 citations
TL;DR: Yeast cells extracted with alkali and acetic acid revealed very thin cell envelopes with prominent bud scars in the shape of a shallow crater with a raised rim, which concluded that chitin is localized in a ring around the bud scar, sandwiched between two layers of glucan.
212 citations
TL;DR: Results of these experiments indicate that the lipase and protease attack the cuticle earlier than the chitinase, which enables penetration of the fungus through the cuticles of the infested insect.
70 citations
32 citations
TL;DR: An inducible lytic enzyme complex from Streptomyces satsumaensis was capable of releasing spherical protoplast-like bodies from the mycelium of Geotrichum candidum and showed the presence of chitinase, mannanase and laminaranase.
Abstract: An inducible lytic enzyme complex from Streptomyces satsumaensis was capable of releasing spherical protoplast-like bodies from the mycelium of Geotrichum candidum. An analysis of this enzyme complex revealed the presence of chitinase, mannanase and laminaranase. Also a combination of chitinase and exolaminaranse from other sources could produce the “protoplasts” under standard conditions.
16 citations