Topic
Chitinase
About: Chitinase is a research topic. Over the lifetime, 4690 publications have been published within this topic receiving 161786 citations. The topic is also known as: 1,4-beta-poly-N-acetylglucosaminidase & poly-beta-glucosaminidase.
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TL;DR: Optimization of physico-chemical parameters, nutritional parameters and studies on the degradation of colloidal chitin to N-acetyl-D-glucosamine showed improved efficiency when endochitinase and chitobiase were used in combination.
62 citations
62 citations
Journal Article•
TL;DR: Chitinase produced by P. illinoisensis KJA-424 caused the lysis of M. incognita eggshell and resulted in the inhibition of egg hatching in vitro, and chitinolytic activity significantly increased for the first 2 days of incubation.
Abstract: A bacterium having strong chitinolytic activity on 02% colloidal chitin-containing agar medium was isolated from coastal soil in Korea Based on the nucleotide sequence of a conserved segment of a 16S rRNA gene, the bacterium was identified as Paenibacillus illinoisensis KJA-424 The population of P illinoisensis KJA-424 and chitinase activity significantly increased for the first 2 days of incubation On SDS-PAGE analysis with 001% glycol chitin, three protein bands (63, 54, and 38 kDa) with chitinolytic activity were detected The effect of P illinoisensis KJA-424 on the egg hatch of root-knot nematode (Meloidogyne incognita) was investigated After 7 days of incubation with the chitinase- producing P illinoisensis KJA-424, none of the eggs hatched, whereas a 398% egg hatching rate was observed in the water control Inverted and scanning electron microscopic observations demonstrated that P illinoisensis KJA-424 deformed and destroyed the eggshell of M incognita In conclusion, chitinase produced by P illinoisensis KJA-424 caused the lysis of M incognita eggshell and resulted in the inhibition of egg hatching in vitro
62 citations
TL;DR: All of the structural and functional properties of AtChiC are quite similar to those obtained for NtChiV, and seem to be common to class V chitinases from higher plants.
Abstract: Expression of a class V chitinase gene (At4g19810, AtChiC) in Arabidopsis thaliana was examined by quantitative real-time PCR and by analyzing microarray data available at Genevestigator. The gene expression was induced by the plant stress-related hormones abscisic acid (ABA) and jasmonic acid (JA) and by the stress resulting from the elicitor flagellin, NaCl, and osmosis. The recombinant AtChiC protein was produced in E. coli, purified, and characterized with respect to the structure and function. The recombinant AtChiC hydrolyzed N-acetylglucosamine oligomers producing dimers from the non-reducing end of the substrates. The crystal structure of AtChiC was determined by the molecular replacement method at 2.0 A resolution. AtChiC was found to adopt an (β/α)8 fold with a small insertion domain composed of an α-helix and a five-stranded β-sheet. From docking simulation of AtChiC with pentameric substrate, the amino acid residues responsible for substrate binding were found to be well conserved when compared with those of the class V chitinase from Nicotiana tabacum (NtChiV). All of the structural and functional properties of AtChiC are quite similar to those obtained for NtChiV, and seem to be common to class V chitinases from higher plants.
62 citations
TL;DR: A chitinase was purified from the culture filtrate of Streptomyces thermoviolaceus OPC-520 and showed high sequence homology with Chitinases from Serratia marcescens QMB1466 and Bacillus circulans WL-12.
Abstract: A chitinase was purified from the culture filtrate of Streptomyces thermoviolaceus OPC-520. The enzyme showed a high optimum temperature (70 to 80 degrees C), a high optimum pH level (8.0 to 10.0), and heat stability. This enzyme showed high sequence homology with chitinases from Serratia marcescens QMB1466 and Bacillus circulans WL-12.
61 citations