Topic
Chitinase
About: Chitinase is a research topic. Over the lifetime, 4690 publications have been published within this topic receiving 161786 citations. The topic is also known as: 1,4-beta-poly-N-acetylglucosaminidase & poly-beta-glucosaminidase.
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TL;DR: The results of this research prove that the substances which are metabolised by S. rimosus the most efficiently are chitosan and the shrimp shell waste, the finest inducer of chitinase synthesis.
58 citations
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TL;DR: A chitinase and a chitosanase were induced from a squid pen powder (SPP)-containing medium of Bacillus cereus TKU030 and purified by precipitation with ammonium sulphate and combined column chromatography and showed high stability in the presence of various surfactants, such as SDS,Tween 20, Tween 40 and Triton X-100.
58 citations
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TL;DR: Chitin metabolism is of high relevance for shrimp growth because it has to be synthesized and cleaved in each molt, based on the need of shrimp to synthesize or hydrolyze chitin from exoskeleton.
58 citations
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TL;DR: Crystal structures of both OfChi-h and its complex with chitoheptaose reveal that OfCha-h possesses a long and asymmetric substrate binding cleft, which is a typical characteristics of a processive exo-chitinase.
58 citations
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TL;DR: A kinetic analysis of the purified mutant chitinases suggests the involvement of both the Asp-197 and AsP-202 residues in the catalytic events of this enzyme, although the effects of mutations of Asp -197 were less severe than those of the other mutations.
Abstract: The contribution of the Asp-197 and Asp-202 residues in chitinase A1 of Bacillus circulans WL-12 to the catalytic reaction was studied by site-directed mutagenesis of these residues. A kinetic analysis of the purified mutant chitinases suggests the involvement of both the Asp-197 and Asp-202 residues in the catalytic events of this enzyme, although the effects of mutations of Asp-197 were less severe than those of the other mutations.
58 citations