Topic
Chitinase
About: Chitinase is a research topic. Over the lifetime, 4690 publications have been published within this topic receiving 161786 citations. The topic is also known as: 1,4-beta-poly-N-acetylglucosaminidase & poly-beta-glucosaminidase.
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TL;DR: A new definition of "exo" and "endo" chitinase that differs from that found in the literature is suggested and is proposed that exochitin enzyme activity be defined as processive action starting at the nonreducing ends of chit in chains with release of successive diacetylchitobiose units.
221 citations
TL;DR: A PR-3 rice chitinase gene (RC7), isolated from R. solani-infected rice plants, was introduced into indica rice cultivars IR72, IR64, IR68899B, MH63, and Chinsurah Boro II by the biolistic and PEG-mediated transformation system and showed different levels of enhanced resistance when challenged with the sheath blight pathogen R.solani.
221 citations
TL;DR: A chitinase-encoding cDNA from the tobacco hornworm, Manduca sexta, is isolated and sequenced, and its sequence is compared with genes encoding chitinolytic enzymes from other sources, and chit inase gene expression and hormonal regulation during the larval-pupal transformation is studied.
219 citations
TL;DR: Accumulation of these proteins, in combination with sugars, appears to constitute a novel, developmentally regulated defense mechanism against phytopathogens in the maturing fruit.
Abstract: During ripening of grape (Vitis labruscana L. cv Concord) berries, abundance of several proteins increased, coordinately with hexoses, to the extent that these became the predominant proteins in the ovary. These proteins have been identified by N-terminal amino acid-sequence analysis and/or function to be a thaumatin-like protein (grape osmotin), a lipid-transfer protein, and a basic and an acidic chitinase. The basic chitinase and grape osmotin exhibited activities against the principal grape fungal pathogens Guignardia bidwellii and Botrytis cinerea based on in vitro growth assays. The growth-inhibiting activity of the antifungal proteins was substantial at levels comparable to those that accumulate in the ripening fruit, and these activities were enhanced by as much as 70% in the presence of 1 M glucose, a physiological hexose concentration in berries. The simultaneous accumulation of the antifungal proteins and sugars during berry ripening was correlated with the characteristic development of pathogen resistance that occurs in fruits during ripening. Taken together, accumulation of these proteins, in combination with sugars, appears to constitute a novel, developmentally regulated defense mechanism against phytopathogens in the maturing fruit.
216 citations
TL;DR: The PGPR mixed bioformulation Pf1+ B. subtilis +neem+chitin was found to be the best for reducing the fruit rot incidence besides increasing the plant growth and yield parameters under both greenhouse and field conditions.
216 citations