Topic
Chitinase
About: Chitinase is a research topic. Over the lifetime, 4690 publications have been published within this topic receiving 161786 citations. The topic is also known as: 1,4-beta-poly-N-acetylglucosaminidase & poly-beta-glucosaminidase.
Papers published on a yearly basis
Papers
More filters
TL;DR: The results indicate that genes so far considered to code for PR proteins may also be involved in the plant response to oxidative stress and Prolonged ozone treatment for 2 days of tobacco Bel W3 led to a persistent level of β-1,3-glucanase and basic chitinase mRNAs, as well as to an increase of acidic chit inase and ‘pathogenesis-related’ (PR) 1b mRNA levels.
Abstract: Treatment of the ozone-sensitive tobacco cultivar Bel W3 with an ozone pulse (0.15 microliter/l, 5 h) markedly increased the mRNA level of basic beta-1,3-glucanase and to a lower degree that of basic chitinase. The increase of beta-1,3-glucanase mRNA level occurred within 1 h and showed a transient maximum. Seventeen hours after ozone treatment, the beta-1,3-glucanase mRNA level decreased to lower values. The increase of basic chitinase mRNA level was delayed and was less pronounced than that of beta-1,3-glucanase mRNA. Cultivar Bel B showed only a small increase of beta-1,3-glucanase mRNA level after the same ozone treatment, whereas its basic chitinase mRNA was more strongly induced. Prolonged ozone treatment for 2 days of tobacco Bel W3 led to a persistent level of beta-1,3-glucanase and basic chitinase mRNAs, as well as to an increase of acidic chitinase and 'pathogenesis-related' (PR) 1b mRNA levels. The results indicate that genes so far considered to code for PR proteins may also be involved in the plant response to oxidative stress.
125 citations
TL;DR: A combination of glycol chitin and aniline blue plate assays correctly identified all in planta inducers of systemic resistance as measured by control of Cercospora leaf spot in classical challenge assays for systemic acquired resistance without the inclusion of false positive identifications, reducing the workload in subsequent disease challenge Assays by nearly 70%.
124 citations
124 citations
TL;DR: A pathogenesis-related protein was found in both the infected and the uninfected leaves of cucumber plants inoculated on the first true leaf with a fungal, a bacterial or a viral pathogen.
124 citations
TL;DR: The intercellular washing fluid from leaves of sugar beet contains a number of proteins exhibiting in vitro antifungal activity against the devastating leaf pathogen Cercospora beticola, and one of these, designated IWF4, was identified, which has a strong affinity to chitin.
Abstract: The intercellular washing fluid (IWF) from leaves of sugar beet (Beta vulgaris L.) contains a number of proteins exhibiting in vitro antifungal activity against the devastating leaf pathogen Cercospora beticola (Sacc.). Among these, a potent antifungal peptide, designated IWF4, was identified. The 30-amino-acid residue sequence of IWF4 is rich in cysteines (6) and glycines (7) and has a highly basic isoelectric point. IWF4 shows homology to the chitin-binding (hevein) domain of chitin-binding proteins, e.g. class I and IV chitinases. Accordingly, IWF4 has a strong affinity to chitin. Notably, it binds chitin more strongly than the chitin-binding chitinases. A full-length IWF4 cDNA clone was obtained that codes for a preproprotein of 76 amino acids containing an N-terminal putative signal peptide of 21 residues, followed by the mature IWF4 peptide of 30 residues, and an acidic C-terminal extension of 25 residues. IWF4 mRNA is expressed in the aerial parts of the plant only, with a constitutive expression in young and mature leaves and in young flowers. No induced expression of IWF4 protein or mRNA was detected during infection with C. beticola or after treatment with 2,6-dichloroisonicotinic acid, a well-known inducer of resistance in plants.
123 citations