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Showing papers on "Chlorococcum published in 2000"


Journal ArticleDOI
TL;DR: This strain represents a potential source of lutein, a commercially interesting carotenoid of application in aquaculture and poultry farming, as well as in the prevention of cancer and diseases related to retinal degeneration.

293 citations


Journal ArticleDOI
TL;DR: The results show that despite of the lower carotenoidcontent as compared with Haematococcus, Chlorococcum can be apotentially interesting strain for secondarycarotenoids production because of its higher growthrate.
Abstract: Changes in the in vivo chlorophyll fluorescencequenching, photosynthesis and pigment composition werefollowed in the green alga Chlorococcum sp.during exposure of the culture to nitrogen deficiencyand salinity stress with the aims to study theinterrelations between changes in physiological andphotochemical parameters and xanthophyll-cyclepigments content during adaptation to stress, and toevaluate the capacity of this green alga to producesecondary carotenoids in tubular photobioreactors.Exposure of Chlorococcum to nitrogendeficiency, 0.2 M NaCl and high irradiance outdoorscaused a strong depression of the photosyntheticactivity and of photochemical quantum yield ofPSII (Fv/Fm). These changes wereaccompanied by an increase of the non-photochemicalquenching coefficient (NPQ), of the amount ofxanthophyll-cycle pigments and of thecarotenoid/chlorophyll ratio. As a result of exposureto stress conditions, cell division completelystopped, although an increase in the biomass dryweight could be detected due to an increase in thecell size. These processes were followed, with acertain delay (15–20 h), by massive appearance ofsecondary carotenoids that reached the maximum (about50% total carotenoids) after 2–3 days of cultivation.The results show that despite of the lower carotenoidcontent (2 mg g-1 dry wt) as compared with Haematococcus, Chlorococcum can be apotentially interesting strain for secondarycarotenoid production because of its higher growthrate.

100 citations


Journal ArticleDOI
TL;DR: Results indicate that higher temperature and pH favor the introduction ofhydroxyl group to the corresponding substrates, however, under high oxygen level keto group was relatively easy to be added compared to the addition of hydroxyl.
Abstract: The effects of temperature, pH and oxygen level onsecondary carotenoids (SC) accumulation in Chlorococcum sp were investigated The optimaltemperature, pH and oxygen level for the yields ofsecondary carotenoids and astaxanthin were35 °C, pH 8 and 10% (v/v), respectively Whilethe ratio (R) of products containing hydroxyl group(s) to those containing keto group (s) increased withthe increase of temperature and pH, R value decreasedwith the level of oxygen These results indicate thathigher temperature and pH favor the introduction ofhydroxyl group to the corresponding substrates,however, under high oxygen level keto group wasrelatively easy to be added compared to the additionof hydroxyl

79 citations


Journal Article
TL;DR: The extent to which each parameter to stimulate the formation of SC in the alga were varied and the potentially improved SC production by manipulating the nutrient levels in the modified media were discussed.
Abstract: The freshwater green alga Chlorococcum sp. grew on NH 4 + , NO 3 - , urea, yeast extract, and peptone as the nitrogen source showing similar patterns of growth and secondary carotenoid (SC) production. However, the most suitable nitrogen source for the induction of SC was urea. The effects of nutrient levels (urea, phosphate, sulfate, ferrous iron, and salt) on growth and SC production were studied by varying the concentration of each nutrient in batch cultures. High biomass production was achieved in cultures containing 20-28 mM urea, 4.8-10 mM phosphate, 1.6 mM sulfate, 70 mM NaCl, and 20- 100 μM iron. The highest SC content in biomass was obtained when cultured at 5- 10 mM urea, 1-2 mM phosphate, 1.6 mM sulfate, 170 mM NaCI, and 50 μM iron. The optimum concentrations of nutrients for biomass and for the SC accumulation in biomass were evaluated and the two media for achieving high biomass production and SC production were thus developed. The extent to which each parameter to stimulate the formation of SC in the alga were varied and the potentially improved SC production by manipulating the nutrient levels in the modified media were discussed.

15 citations


Patent
23 May 2000
TL;DR: In this paper, the objective composition for preventing the darkening and browning of food is prepared by using the algae extract in combination with an antioxidant such as Lascorbic acid and/or a metal chelating agent.
Abstract: PROBLEM TO BE SOLVED: To obtain a composition for preventing the darkening and browning of a food, exerting little influence on the color and taste of the food, having high safety and useful for the treatment of fish and shellfish (or its processed product), vegetable (or its processed product), fruit (or its processed product), or the like, by including an extract of algae of e.g. the order Chlorellales. SOLUTION: The objective composition contains an extract of one or more kinds of algae belonging to the order Chlorellales such as Chlorococcum and Characium, the order Ulvales such as Monostroma and Enteromorpha, the order Gigartinales such as Eucheuma and Meristothma or the order Bangiales such as Erythrotrichia and Colaconema. Preferably, the objective composition for preventing the darkening and browning of food is prepared by using the algae extract in combination with an antioxidant such as L-ascorbic acid and/or a metal chelating agent.

2 citations