Chlorococcum

About: Chlorococcum is a research topic. Over the lifetime, 268 publications have been published within this topic receiving 7317 citations.

Biochemical characterization of microalgae collected from north east region of India advancing towards the algae-based commercial production

Abstract: Selection of suitable strain of microalgae is the crucial factor for large-scale production of algae-based products. Efforts have been made here for isolation, identification and biochemical characterization of five microalgae strains collected from Tripura (a small state in north-eastern region of India). Two Chlorococcum sp. (NITAAP008 and NITAAP019) demonstrate their high lipid (15–24%), equal amounts of carbohydrate and protein (35–40%), with specific growth rate of 0.13 day−1. These strains are potential resource for biofuel production. After lipid extraction, remaining biomass can be used as source of carbohydrate for the production of other biofuels. One isolated strain is identified as Chlorella sp. (NITAAP009) and shows 22–33% carbohydrate, 41–50% protein and 5% chlorophyll with specific growth rate of 0.125 day−1. Another Chlorella sp. (NITAAP011) isolated from lake area exhibits significant chlorophyll (5–6.4%), 30–50% carbohydrate, 48–60% protein and low lipid (1–10%) with lower specific growth rate (0.10 day−1). Both strains are having industrial competence for chlorophyll production due to their synthesizing ability of significant amount of chlorophyll (5–6.4%). The last one, Korshikoviella sp. (NITAAP017), has 15–18% lipid, 22–34% carbohydrate, 30–43% protein and 3–4% chlorophyll with specific growth rate of 0.12 day−1 and can be used for food supplement production or lipid synthesis. © 2017 Curtin University of Technology and John Wiley & Sons, Ltd.

Biofixation of carbon dioxide by Chlorococcum sp. in a photobioreactor with polytetrafluoroethene membrane sparger

Abstract: The greenhouse effect, caused by excessive carbon dioxide emissions, constitutes a major aspect of global warming. Biological fixation of carbon dioxide using microalgae is an effective carbon dioxide reduction technology, but its widespread implementation is limited by the poor mass transfer efficiency. In this study, Chlorococcum sp. was cultured in a photobioreactor with a polytetrafluoroethene membrane sparger (PTFE) to study CO 2 biofixation and microalgae growth. Daily variations of dissolved oxygen (DO), pH and dissolved CO 2 were analyzed during batch culture of Chlorococcum sp. in the photobioreactor. The culture of Chlorococcum sp. under different operating conditions, such as pH, light cycle (light:dark) and nitrate feeding, were carried out to optimize the CO 2 biofixation rate and the algal productivity. The results confirmed that the photobioreactor with a membrane sparger is an alternative option for CO 2 removal from flue gas by cultivation of microalgae. Keywords: Bioreactor, polytetrafluoroethene membrane sparger (PTFE) membrane sparger, Chlorococcum sp., greenhouse gas African Journal of Biotechnology Vol. 11(29), pp. 7445-7453, 10 April, 2012

New species of green microalgae (Chlorophycophyta) from an eastern Washington silt loam

Abstract: Five new species of green microalgae are described from axenic cultures isolated from the surface millimetre of a Schumacher silt loam in Whitman County, Washington, U.S.A. They are as follows: Order Chlorococcales—Characium astipitatum sp. nov. (BM-105), Chlorococcum pseudodictyosphaerium sp. nov. (BM-104), Dictyococcus schumacherensis sp. nov. (BM-126). Order Chlorosarcinales—Chlorosarcinopsis amylophila sp. nov. (BM-141). Order Chaetophorales—Pleurastrum pholoheterotrophicum sp. nov. (BM-172). Supplementary characteristics are quantified in addition to traditional morphological descriptions. The properties included are form and colour of the plant-mass, differential utilization of nitrogen and carbon sources, growth in complex bacteriological media, production of extracellular enzymes, and tolerance to antibiotics and crystal violet.

An alternative high-throughput staining method for detection of neutral lipids in green microalgae for biodiesel applications

Abstract: A simple and high-throughput method for determining in situ intracellular neutral lipid accumulation in Chlorella ellipsoidea and Chlorococcum infusionum with flow cytometry and confocal microscopy was established by employing different solvents and a lipophilic dye, Nile red. Seven different organic solvents, acetic acid, dimethyl sulfoxide (DMSO), acetone, methanol, ethanol, n-hexane, and chloroform at different concentrations ranging from 0 to 80% (v/v) were tested. The fluorescence signal for neutral lipids was collected with a 586/42 emission filter (PE-A) and the maximum fluorescence intensity (% grandparent) was measured as 74.01 ± 4.82% for Chlorella and 70.1 ± 5.52% for Chlorococcum at 30% acetic acid (v/v). The statistical analysis of Nile red-stained cells showed a high coefficient of variation (CV), standard deviation (SD), mean, and median values in the acetic acid-based staining method, followed by DMSO, n-hexane and chloroform. Confocal microscopy revealed a high rate of accumulation of cytosolic neutral lipids when stained with Nile red and other organic solvents. Higher lipid accumulation in Fesupplemented conditions was also detected and a maximum lipid content of 57.36 ± 0.41% (4-fold) in Chlorella and 48.20 ± 0.43% (4-fold) in Chlorococcum were measured at 0.001 g/L of ferrous sulfate (FeSO4). High fluorescence intensity (75.16 ± 0.24% in Chlorella and 72.24 ± 1.07% in Chlorococcum) in Fe-treated cells confirmed the efficiency of the staining procedure.

Decolorization of dyestuffs by some species of green algae and cyanobacteria and its consortium

Abstract: Synthetic dyes are scattered in untreated or inappropriately treated effluents, and their dangerous items created during the halfway corruption are released into the water bodies that cause a horrendous smell, which prompts anomalous changes in the nature of water. In the present study, green algae and cyanobacteria are considered as a significant hotspot for decolorizing color and material gushing. Chlorococcum sp., Scenedesmus obliquus, Chlorella vulgaris, and Oscillatoria sp. were investigated for degradation and removal of some azo dyes [Reactive Orange 122 (Orange 2RL) and Reactive Red 194 (Reactive Red M-2BF)]. The results showed that the maximum decolorization was spotted at 20 ppm Reactive Orange 122 with Oscillatoria sp. mixed with S. obliquus (98.54%). 20 ppm Reactive Red 194 was decolorized by Oscillatoria sp. mixed with S. obliquus (97.58%) after 7 days of incubation. The decolorization was detected by spectroscopic analysis and Fourier transformed infrared (FTIR) spectroscopy. The suitable factors that accelerated the azo dye decolorization and enhanced the biological treatment methods to be more effective and speedier in decolorization were investigated. At 25 °C and continuous lighting, the highest percentage of the azo dye decolorization was obtained; BG11 was the suitable medium that gives a high percentage of the azo dye decolorization. However, relative to the effect pH on azo dye decolorization, results show pH 11 and pH 9 more effective on azo dye decolorization for Reactive Orange 122 and Reactive Red 194, respectively. A total of 6% of thiamine and ascorbic acid recorded maximum degradation activity at Reactive Orange 122 when treated with Oscillatoria sp. mixed with S. obliquus 79.13% and 77.18%, respectively.