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Chlorococcum

About: Chlorococcum is a research topic. Over the lifetime, 268 publications have been published within this topic receiving 7317 citations.


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Journal ArticleDOI
TL;DR: In this article, a laboratory-based integrated approach was undertaken for improvement of lipid accumulation in green microalgae under sodium chloride (NaCl) stress, and the maximum biomass yields were obtained in the media with initial NaCl concentrations at 2 g L−1 (for Chlorella) and 0.05 g L −1 (For Chlorococcum) after 14 days of culture.
Abstract: A laboratory based integrated approach was undertaken for improvement of lipid accumulation in green microalgae under sodium chloride (NaCl) stress. The maximum biomass yields (1.22 ± 0.04 g L−1 in Chlorella and 1.31 ± 0.04 g L−1 in Chlorococcum) were obtained in the media with initial NaCl concentrations at 2 g L−1 (for Chlorella) and 0.05 g L−1 (for Chlorococcum) after 14 days of culture. Interestingly, maximum lipid accumulation obtained was 45.8 ± 0.4% w/v for Chlorella (on day eight) and 36.33 ± 0.56% w/v for Chlorococcum (on day ten) at an exposure of 5 g L−1 and 1.5 g L−1 NaCl concentrations, respectively. Fatty acid profiles of methyl esters for neutral and polar lipid fractions showed the presence of myristic (C14:0), palmitic (C16:0), palmitoleic (C16:1), oleic (C18:1) and linoleic (C18:2) acids as major fatty acids. Fluorescence intensities (% positive Nile red stained cells) of neutral and polar lipid fractions were also higher in NaCl stress conditions. A significant correlation between polar lipid and neutral lipid was found in both Chlorella (r2 = 0.979) and Chlorococcum (r2 = 0.972). Flow cytometric study demonstrated a positive fluorescence signal for neutral and polar lipid bodies related to Nile red fluorescence. Associated biochemical changes, like those in total chlorophyll, carotenoid, carbohydrate and protein contents, were determined under variable NaCl concentrations. The elemental compositions (CHNS) and CO2 fixation rate were assessed using high lipid yielding cells under optimum NaCl concentrations. Biodiesel fuel properties were obtained from high lipid yielding cells of the test microalgae and compared with Indian and international diesel standards. Finally large-scale cultivation of Chlorella ellipsoidea was performed in a hybrid photobioreactor (800 L capacity) and the highest biomass and lipid content obtained was 1.66 ± 0.09 g L−1 (at 8 days) and 54.73 ± 1.78% w/v (at 10 days) at 5 g L−1 NaCl respectively. This optimized NaCl concentration makes it an economical substitute for biodiesel production on a large scale.

11 citations

Journal ArticleDOI
TL;DR: It is proposed that pigmentation of old cultures be used as the first criterion for distinguishing species of Chlorococcum.
Abstract: Eighteen isolates of Chlorococcum were incubated under defined culture conditions for 6-7 weeks. At that time, isolates were separated into green, yellow-green, orange, or red color groups according to the color of the cultures. Total chlorophyll, carotenoid concentration, and percentage of carotenoids in relation to total pigment content were measured for each species. Individual carotenoids of each isolate were identified and quantified. Each color group had pigment characteristics which were distinctive. Other studies on Chlorococcum are discussed in relation to this work. It is proposed that pigmentation of old cultures be used as the first criterion for distinguishing species of Chlorococcum.

11 citations

Journal ArticleDOI
TL;DR: The present study suggested that the four isolates are good for biodiesel production.
Abstract: Twenty-five (25) strains of microalgae were isolated and screened for growth, lipid accumulation and biodiesel production from arid environments of North East Nigeria. Isolates that produced biomass concentration (≥ 1.50 g L -1 cell dry weight), accumulated high concentrations of lipids (≥ 18% of the cell biomass) and could be purified on agar plates were selected for further studies. Four strains morphologically identified as Oocystis IA1, Chlorella IA7, Chlorococcum KA9 and Botyrococcus YA5 were selected. The maximum biomass concentrations (g L -1 ) and lipid contents (%) were 3.02, 3.92, 3.59 and 2.89, and 57, 67.23, 63 and 65 dry cell weights for Oocystis IA1, Chlorella IA7, Chlorococcum KA9 and Botyrococcus YA5, respectively. The highest oil productivities (mg L -1 day -1 ) were 135.28 ± 3.32 ( Oocystis IA1), 165.22 ± 3.36 ( Chlorella IA7), 131.76 ± 1.11 ( Chlorococcum KA9) and 140.37 ± 2.13 ( Botyrococcus YA5). Maximum chlorophyll contents (g g -1 cell) were 34.97, 30.00, 39.71 and 32.27, respectively. Fatty acid methyl ester profiles indicated the presence of C16:0, C16:1, C18:0, C18:1, and C18:2. Oleic acid (C18: 1) was predominant, ranging between 73.3 and 85.6%. Biodiesel properties were within the ASTM standards. The present study suggested that the four isolates are good for biodiesel production. Keywords: Biodiesel, Chlorella , Chlorococcum , Botyrococcus sp., lipid accumulation, microalgae, Oocystis . Abbreviation: FAME, Fatty acids methyl esters.

11 citations

Journal ArticleDOI
TL;DR: Proteins of whole cell extracts from 16 Chlorococcum species and 3 Tetracystis species were analyzed for isozymes of nonspecific α‐esterases, leucine aminopeptidase, and malate dehydrogenase using starch gel electrophoresis.
Abstract: SUMMARY Proteins of whole cell extracts from 16 Chlorococcum species and 3 Tetracystis species (formerly Chlorococcum) were analyzed for isozymes of nonspecific α-esterases, leucine aminopeptidase, and malate dehydrogenase using starch gel electrophoresis. Each species could be identified by the banding patterns. Chlorococcum species are heterogenous regarding the presence, number, electrophoretic mobility, concentration, and distribution of isozymes. The data are discussed in relation to current knowledge of each species. Taxonomic applications of algal isozymes are considered.

11 citations

Journal Article
TL;DR: Results showed that different initial pH values had slight effects on growth of algae,because pH values changed to about 10.0 after 4 days of cultivation,but different constant pH had marked effect ongrowth of algae.
Abstract: Growth of algae at different initial and constant pH values was studied with bulk culture.Initial pH value in BG11 culture medium was adjusted to 6.0,7.0,8.0,9.0 and 10.0 with either 0.5mol/L NaOH or 0.5mol/L HCL.Constant pH values were kept by addition of 20mmol/L bicine.Results showed that different initial pH values had slight effects on growth of algae,because pH values changed to about 10.0 after 4 days of cultivation,but different constant pH had marked effect on growth of algae.Optimal pH values for Microcystis aeruginosa,Anabaena flos-aquae and Oscillatoria planctonica were 9.0, 8.0~9.0 and 7.0~8.0 respectively while for Scendesmus obliquus,Chlorococcum sp and Chlamydomonas reinhardtii were 9.0~ 10.0,7.0~8.0 and 7.0 respectively.

10 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20221
202121
202013
201923
201812
201714