Chlorococcum

About: Chlorococcum is a research topic. Over the lifetime, 268 publications have been published within this topic receiving 7317 citations.

In vitro optimization for enhanced cellulose degrading enzyme from Bacillus licheniformis KY962963 associated with a microalgae Chlorococcum sp. using OVAT and statistical modeling

Abstract: Cellulase has gained interest in researchers due to its great applicability in the field of biotechnology. The Bacillus licheniformis KY962963 used in the study was isolated from Chlorococcum sp. Optimization of various physical parameters like pH, temperature, salinity and effect of co-inducers on the cellulase production (FPase and CMCase) was carried out. In crude enzyme extracts, the selected bacterial strain exhibited higher endoglucanase (CMCase) activity as compared to total cellulase activity (FPase). The highest production of cellulase was obtained with Bacillus licheniformis KY962963 when 1% (w/v) cellulose and 0.5% (w/v) peptone as co-inducers was used at 37 °C, pH 7.0, 72 h of production time where CMCase activity was found to be of 7.72 IU/ml and FPase 6.19 IU/ml. The conditions were optimized using OVAT approach which were further validated through statistical modeling. The methods used in this study has established its suitability to optimize cellulase production for its industrial applicability.

Reproductive rate of Brachionus calyciflorus under the influence of salinity, temperature, feed type and feed concentration

Abstract: The impact of salinity, temperature, feed type and feed concentration on reproductive rate ‘r’ of Brachionus calyciflorus were examined in the present study. Three salinity levels (0.5%, 5.0% and 10.0%) and three temperature regimes (25 ± 1 oC, 29 ± 1 oC and 35 ± 1 oC) were evaluated. Four microalgal diets, Chlorella ellipsoidea, Ankistrodesmus convolutes, Scenedesmus protuberans and Chlorococcum infusorium at a concentration of 1x 106, 2x 106, 3x 106, 4x 106, 6x 106, 8x 106, 10x 106 and 12x 106 were tested in the experiment. The findings showed that all these parameters have significant influence on reproductive potential of Brachionus calyciflorus. The optimum salinity and temperature required for rmax for B. calyciflorus were of 0.5% and 29 ± 1 oC, respectively. The intrinsic rate of reproduction of B. calyciflorus for the best salinity x feed concentration with C. ellipsoidea (8 x 106 cell mL-1), A. convolutus (6 x 106 cells mL-1), C. infusorium (8 x 106 cells mL-1) and S. protuberans (4 x 106cells mL-1 ) at the optimal salinity and temperature were 1.91, 1.83, 1.77 and 1.62. Based on the study, the maximum reproductive potential of B. calyciflorus can be achieved by feeding with C. ellipsoidea at a salinity and temperature of 0.5% and 29 ± 1 oC, respectively for the larviculture of fishes.

Isolation and identification of lipid-producing microalgae of Uzbekistan

Abstract: From water samples of the Syr-Darya andAmu-Darya are isolated 15 local microalgae belonging to the genus Chlorococcum and 13 to the genus ScenedesmusAir-dry biomass ofmicroalgae ranged from129 to 279 g/l of environment The lipid content of effective strains of Chlorococcum sp4, Chlorococcum sp8, Chlorococcum sp12 and Chlorococcum sp37 was 418 - 580%of the total dry biomass ofmicroalgaeHigh lipid accumulation observed in cells of cultures Scenedesmus sp29 and Scenedesmus sp37- 40,3%and 48,0%, respectively