Chlorococcum

About: Chlorococcum is a research topic. Over the lifetime, 268 publications have been published within this topic receiving 7317 citations.

The effects of Zn2+ stress on the growth, physiological characteristics and cell structure of Chlorococcum sp.

Abstract: In the Zn2+ stress experiments, the concentrations of Zn2+ were 0.1, I, 10, 50, 100, 200 and 400 mg/L, BG11 culture medium was served as the control. The results showed that Zn2+ affected markedly on the growth, physiological characteristics and cell structure of Chlorococcum sp. under certain concentration. When the concentration of Zn2+ was 0.1-1mg/L, the growth of Chlorococcum sp. showed no obvious difference compared with the control. When the concentration of Zn2+ was 10-50mg/L, Chlorococcum sp. could maintain certain growth rate yet. When the concentration of Zn2+ was higher than 100mg/L, the growth of Chlorococcum sp. was inhibited markedly. The contents of Chl alpha + Chl b and Chl a decreased gradually with the increase of the concentrations of Zn2+ in the medium. When the concentration of Zn2+ was less than 10mg/L, the photosynthesis and respiration of Chlorococcum sp. increased gradually with the increase of Zn2+ concentrations; and when the concentration of Zn2, was higher than 10mg/L, they decreased gradually with the increase of Zn2+ concentrations. The content of malondiadehyde and activity of peroxidase increased gradually with the increase of Zn2+ concentrations, and the activities of eatalase and superoxide dismutase increased at first and later decreased with the increase of Zn2+ concentrations. Compared with the cell cultured in BG11, the cell cultured in low concentrations (<= 10mg/L) showed few changes in pigment and thickness of cell wall. The cell wall of cell cultured in high concentrations of Zn2+ became thicker, and the pigment decreased and the pyrenoid disappeared. When the concentration of Zn2+ was <= 10mg/L, the removal rate of Chlorococcum sp. on Zn2+ reached the maximum and was 100%, and it was above 80% under the concentrations that Chlorococcum sp. could maintain the growth. The results demonstrated that the Chlorococcum sp. could be applied to the treatment of wastewater containing Zn2+ because the Chlorococcum sp. could endure the stress of Zn2+ and was of high removal rate on Zn2+.

Production of astaxanthin-rich chlorophyceae

Abstract: PURPOSE:To obtain the subject chlorophyceae in a simple culture process by making a culture of the chlorophyceae capable of bio-synthesis of astaxanthin in a culture medium containing sodium-, potassium- and/or rabidium-salts. CONSTITUTION:The objective chlorophyceae can be obtained by making a culture of the chorophyceae capable of biosynthesis of astaxanthin in a culture medium containing 0.2-1M of at least one kind of sodium salts, potassium salts and rabidium salts. The chlorophyceae to be used as the starting material is, e.g., Chlamydomonas, Hematocaccus, Ankistrodesmus, Chlorella, Chlorococcum, Coelastrum, Crucigenia, Dictyococcus, Scenedesmus, Scotiella, Protosiphon. Among then, e.g., Chlorella and Scenedesmus have already been used as foods, thus being free from any problem in respect of safety. Moreover, for the above Chlorophyceae, mass culture technology has been established, therefore, they are preferably applicable to the present production process.

Environmental Factors Associated with the Eukaryotic Microbial Community and Microalgal Groups in the Mountain Marshes of South Korea.

Abstract: The diversity indices of eukaryotic microalgal groups in the Jeonglyeongchi, Waegok, and Wangdeungjae marshes of Mount Jiri, Korea, were measured using Illumina MiSeq and culture-based analyses. Waegok marsh had the highest species richness, with a Chao1 value of 828.00, and the highest levels of species diversity, with Shannon and Simpson index values of 6.36 and 0.94, respectively, while Wangdeungjae marsh had the lowest values at 2.97 and 0.75, respectively. The predominant species in all communities were Phagocata sibirica (Jeonglyeongchi, 68.64%), Aedes albopictus (Waegok, 34.77%), Chaetonotus cf. (Waegok, 24.43%), Eimeria sp. (Wangdeungjae, 26.17%), and Eumonhystera cf. (Wangdeungjae, 22.27%). Relative abundances of the microalgal groups Bacillariophyta (diatoms) and Chlorophyta (green algae) in each marsh were respectively: Jeonglyeongchi 1.38% and 0.49%, Waegok 7.0% and 0.3%, and Wangdeungjae 10.41% and 4.72%. Illumina MiSeq analyses revealed 34 types of diatoms and 13 types of green algae. Only one diatom (Nitzschia dissipata) and five green algae (Neochloris sp., Chlamydomonas sp., Chlorococcum sp., Chlorella vulgaris, Scenedesmus sp.) were identified by a culture-based analysis. Thus, Illumina MiSeq analysis can be considered an efficient tool for analyzing microbial communities. Overall, our results described the environmental factors associated with geographically isolated mountain marshes and their respective microbial and microalgal communities.

Immature symbiotic system between horizontally transmitted green algae and brown hydra

Abstract: Some strains of brown hydra (Hydra vulgaris) are able to harbor the green algae Chlorococcum in their endodermal epithelial cells as symbionts However, the relationship between brown hydra and chlorococcum is considered to be incipient symbiosis because most artificially introduced symbionts are not stable and because symbiotic H vulgaris strains are rare in the wild In this study, we compared the gene expression levels of the newly established symbiotic hydra (strain 105G), the native symbiotic strain (J7), and their non-symbiotic polyps to determine what changes would occur at the early stage of the evolution of symbiosis We found that both the 105G and J7 strains showed comparable expression patterns, exhibiting upregulation of lysosomal enzymes and downregulation of genes related to nematocyte development and function Meanwhile, genes involved in translation and the respiratory chain were upregulated only in strain 105G Furthermore, treatment with rapamycin, which inhibits translation activity, induced the degeneration of the symbiotic strains (105G and J7) This effect was severe in strain 105G Our results suggested that evolving the ability to balance the cellular metabolism between the host and the symbiont is a key requirement for adapting to endosymbiosis with chlorococcum

Removal Efficiency of Nitrogen and Phosphorus in Wastewater by Two Species of Algae

Abstract: By analyzing the concentration changes of nitrogen and phosphorus in experimental water,both a laboratory study and an outdoor experiment have been conducted for testing the removal efficiency of nutrients by algae.The test alga for the laboratory study is Chlorococcum sp.and for the field experiment is Cladophora oligoclona Kutz.respectively.The results show that Chlorococcum sp.grow quite well and have high removal rates of nitrogen and phosphorus in the sewage with very high concentrations of nitrogen and phosphorus.The removal rates of total soluble nitrogen,nitrate,ammonia and total soluble phosphorus from synthetic sewage were 46.2%,37.8%,98.4% and 79.3% in six days.The removal rate of total soluble phosphorus from crude lake water by the alga was 79.2% in five days.Cladophora oligoclona can grow normally and absorb relatively on nitrogen and phosphorus from the natural water in outdoor conditions.It plays an important role on reducing the specific conductance and improving the water quality.According to the results obtained in this study and referring to other relational research reports,both Chlorococcum sp.and Cladophora oligoclona have ability for removing nutrients from wastewater.Chlorococcum sp.is suitable for rehabbing and improving in static water and Cladophora oligoclona is suitable for treating and protecting in flowing water.