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Chromosome

About: Chromosome is a research topic. Over the lifetime, 17538 publications have been published within this topic receiving 660077 citations. The topic is also known as: chromosomes & GO:0005694.


Papers
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Journal ArticleDOI
10 Aug 1995-Genomics
TL;DR: The isolation of two YAC clones that map to chromosome 14 and that contain multiple copies of a D4Z4-like repeat is described and the name 3.3-kb repeat is proposed for this family of repetitive sequence elements.

153 citations

Journal ArticleDOI
TL;DR: Metaphase chromosomes of three cervical cancer cell lines were subjected to in situ hybridizations with the DNA of human papillomaviruses (HPV) types 16 and 18 and indirectly support a trans-acting function of HPV-mediated cell transformation.
Abstract: Metaphase chromosomes of three cervical cancer cell lines (HeLa, CasKi, SiHa) were subjected to in situ hybridizations with the DNA of human papillomaviruses (HPV) types 16 and 18, respectively. Previous studies have demonstrated multiple copies of HPV 18 DNA in HeLa and of HPV 16 DNA in CasKi cells, but only 1-2 HPV 16 copies in cells of the SiHa line. The viral DNA persists in an integrated state (Schwarz et al 1985). Analysis of the integration sites revealed at least 11 chromosomal sites of HPV 16 integration in CasKi cells. SiHa cells contain integrated HPV 16 DNA in the region q21-q31 of chromosome No. 13. In HeLa cells integration of HPV 18 occurred in chromosome No. 8, band q24. Thus, no evidence was obtained for the existence of preferential chromosomal regions for HPV integration. The data indirectly support a trans-acting function of HPV-mediated cell transformation.

153 citations

Journal ArticleDOI
11 Mar 1994-Cell
TL;DR: It is demonstrated that the chromosomes undergo a dramatic structural reorganization prior to synapsis in zygotene, and the specialized prezygotene chromosome morphology may facilitate homology recognition once the homologs have been brought together.

153 citations

Journal ArticleDOI
TL;DR: This is the first demonstration of how a complete set of mouse chromosome paints can be produced, separated using flow cytometry into 18 distinguishable peaks.
Abstract: Using peripheral blood lymphocyte cultures and duallaser flow cytometry, we have routinely obtained high-resolution bivariate flow karyotypes of the dog in which 32 peaks are resolved. To allow the identification of the chromosome types in each peak, chromosomes were flow sorted, amplified and labelled by polymerase chain reaction with partially degenerate primers and hybridized onto metaphase spreads of a male dog. The chromosome paints from 22 of the 32 peaks each hybridized to single homologue pairs and eight peaks each hybridized to two pairs. Paints from the remaining two peaks hybridized to only one homologue each in the male metaphase spread, thus corresponding to the sex chromosomes X and Y. All of the 38 pairs of autosomes and the two sex chromosomes of the dog could be accounted for in these painting experiments. The positions of chromosomes 1–21 were assigned to the flow karyotype (only chromosomes 1–21 have as yet been officially designated). The high-resolution flow karyotype and the chromosome paints will facilitate further standardization of the dog karyotype. The ability to sort sufficient quantities of dog chromosomes for the production of chromosome-specific DNA libraries has the potential to accelerate the physical and genetic mapping of the dog genome.

153 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20241
2023862
20221,198
2021368
2020359
2019365