Showing papers on "Chrysanthemum indicum published in 2018"

Highly Oxidized Guaianolide Sesquiterpenoids with Potential Anti-inflammatory Activity from Chrysanthemum indicum

Abstract: Ten new highly oxidized monomeric (1–8) and dimeric guaianolides (9 and 10), along with two known guaianolide derivatives (11 and 12), were isolated from the aerial parts of Chrysanthemum indicum using a bioassay-guided fractionation procedure. The new compounds were characterized by the basic analysis of the spectroscopic data obtained, and the absolute configurations were determined by both empirical approaches and ECD calculations. Inhibitory effects of 1–12 on nitric oxide production were investigated in lipopolysaccaride (LPS)-mediated RAW 264.7 cells, and most of them (1–8 and 11) displayed IC50 values in the range 1.4–9.7 μM. Moreover, a mechanistic study revealed that the potential anti-inflammatory activity of compound 1 appears to be mediated via suppression of an LPS-induced NF-κB pathway and down-regulation of MAPK activation.

SSR marker development and intraspecific genetic divergence exploration of Chrysanthemum indicum based on transcriptome analysis

Abstract: Chrysanthemum indicum L., an important ancestral species of the flowering plant chrysanthemum, can be used as medicine and for functional food development. Due to the lack of hereditary information for this species and the difficulty of germplasm identification, we herein provide new genetic insight from the perspective of intraspecific transcriptome comparison and present single sequence repeat (SSR) molecular marker recognition technology. Through the study of a diploid germplasm (DIWNT) and a tetraploid germplasm (DIWT), the following outcome were obtained. (1) A significant difference in Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations for specific homologous genes was observed using the OrthoMCL method for the identification of homologous gene families between the two cytotypes. Ka/Ks analysis of common, single-copy homologous family members also revealed a greater difference among genes that experienced positive selection than among those experiencing positive selection. (2) Of more practical value, 2575 SSR markers were predicted and partly verified. We used TaxonGap as a visual tool to inspect genotype uniqueness and screen for high-performance molecular loci; we recommend four primers of 65 randomly selected primers with a combined identification success rate of 88.6% as priorities for further development of DNA fingerprinting of C. indicum germplasm. The SSR technology based on next-generation sequencing was proved to be successful in the identification of C. indicum germplasms. And the information on the intraspecfic genetic divergence generated by transcriptome comparison deepened the understanding of this complex species’ nature.

Effect of Volatile Organic Chemicals in Chrysanthemum indicum Linné on Blood Pressure and Electroencephalogram.

Abstract: This study identified the volatile organic compounds in the essential oils that are extracted from Chrysanthemum indicum Linne (C. indicum Linne) and investigated the effects of the inhalation of these compounds. We detected a total of 41 volatile organic compounds, including 32 hydrocarbons, four acids, three alcohols, two ketones, and one aldehyde. In a sniffing test, seven types of volatile organic compounds were identified. Furthermore, the volatile organic compounds in C. indicum Linne that were identified were found to be derived from 1,8-cineole and camphor. After inhalation of the essential oils, the subjects' systolic blood pressure and heart rate decreased. This indicates that inhalation of the essential oils extracted from C. indicum Linne provides mental and physical relaxation. We examined the changes in electroencephalogram findings that are observed after C. indicum Linne essential oil inhalation. An increase in theta and alpha waves, which usually appear during relaxation, as well as a decrease in beta and gamma waves, which appear during brain activity such as excessive attention, were noted. These results indicate that C. indicum Linne essential oil inhalation helps to reduce blood pressure and may provide mental and physical relaxation.

DKB114, A Mixture of Chrysanthemum Indicum Linne Flower and Cinnamomum Cassia (L.) J. Presl Bark Extracts, Improves Hyperuricemia through Inhibition of Xanthine Oxidase Activity and Increasing Urine Excretion.

Abstract: Chrysanthemum indicum Linne flower (CF) and Cinnamomum cassia (L.) J. Presl bark (CB) extracts have been used as the main ingredients in several prescriptions to treat the hyperuricemia and gout in traditional medicine. In the present study, we investigated the antihyperuricemic effects of DKB114, a CF, and CB mixture, and the underlying mechanisms in vitro and in vivo. DKB114 markedly reduced serum uric acid levels in normal rats and rats with PO-induced hyperuricemia, while increasing renal uric acid excretion. Furthermore, it inhibited the activity of xanthine oxidase (XOD) in vitro and in the liver in addition to reducing hepatic uric acid production. DKB114 decreased cellular uric acid uptake in oocytes and HEK293 cells expressing human urate transporter (hURAT)1 and decreased the protein expression levels of urate transporters, URAT1, and glucose transporter, GLUT9, associated with the reabsorption of uric acid in the kidney. DKB114 exerts antihyperuricemic effects and uricosuric effects, which are accompanied, partially, by a reduction in the production of uric acid and promotion of uric acid excretion via the inhibition of XOD activity and reabsorption of uric acid. Therefore, it may have potential as a treatment for hyperuricemia and gout.

Chrysanthemum indicum L. ethanol extract reduces high-fat diet-induced obesity in mice.

Abstract: The present study was undertaken to investigate the mechanism behind the anti-obesity effect of the 50% ethanol extract of Chrysanthemum indicum L. flowers (CIEE) in a mouse model of high-fat diet (HFD)-induced obesity. Male C57BL/6J mice (six mice in each group) were administered CIEE (8, 40 and 200 mg/kg) for 6 weeks while being fed with a HFD. Garcinia cambogia (GC) was used as the positive control and was administered in the same manner as CIEE. Results demonstrated that oral administration of CIEE significantly reduced body weight, epididymal white adipose tissue (EWAT), liver weight and serum levels of total cholesterol and triglyceride (P<0.05). In addition, CIEE reduced serum leptin and increased adiponectin levels. CIEE significantly downregulated peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein-α and fatty acid synthase expression levels in EWAT, and upregulated the protein expression of PPARα in liver tissue of HFD-fed obese mice (P<0.05). These results suggested that Chrysanthemum indicum L. flowers may be a potentially effective therapeutic agent for obesity and its associated complications.

Chrysanthemum indicum microparticles on removal of hazardous Congo red dye using response surface methodology

Abstract: Biomass-derived adsorbents have been intensively studied due to their competence in reducing pollutants with conventional methods. Flowers are used as source in medicine, cosmetics and even as adsorbents for pollutant abatement. In this study, the microparticles from Chrysanthemum indicum were used as adsorbent for reducing the Congo red dye concentration from synthetic solution. Batch trials were evaluated to understand the influence of factors and optimization was carried out using central composite design. Maximum reduction (84.1%) achieved under the optimized settings of pH (1.0), adsorbent dose (300 ppm), stirring speed (150 rpm), and contact time (75 min) at initial dye concentration (150 ppm at 30 °C). Microparticle size ensured with surface morphology of the adsorbent using electron microscopy and functional groups was studied using infrared spectroscopy techniques, respectively. Regression coefficient (R2) value was obtained as 0.956 which indicates that the predicted values were in good agreement with their corresponding experimental values for the Congo red dye adsorption. Based on the investigation, it is inferred that the Chrysanthemum indicum flower has the potential for Congo red dye reduction from aqueous solution.

A 1-deoxy-d-xylulose 5-phosphate reductoisomerase gene probably involved in the synthesis of terpenoids in Chrysanthemum indicum var. aromaticum

Abstract: Terpenoids are important secondary metabolites in plants. The synthesis of terpenoids involves a key enzyme, 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXR). In an aromatic ornamental plant, ...

Peroxisome proliferator-activated receptor-γ agonistic effect of Chrysanthemum indicum capitulum and its active ingredients

Abstract: Objective: The capitulum of Chrysanthemum indicum (Compositae) is the source of a crude drug used in Japanese traditional Kampo and traditional Chinese medicine. Previous research showed that C. indicum flowers promote adipocyte differentiation through the activation of peroxisome proliferator-activated receptor (PPAR)-γ that may be an important mechanism for controlling systemic insulin resistance or other biological functions. Materials and Methods: The capitulum of C. indicum was extracted with methanol, and its PPAR-γ agonistic activity was measured using luciferase assay. The active ingredients were isolated by the activity-guided fractionations. Results: We isolated (Z)-tonghaosu and (E)-tonghaosu, and (E)-tonghaosu had PPAR-γ agonistic constituents by the activity-guided fractionation from the capitulum of C. indicum. The isomer (Z)-tonghaosu did not show PPAR-γ-agonistic activity. Conclusion: (E)-tonghaosu is one of the active ingredients as PPAR-γ agonist in the capitulum of C. indicum. Abbreviations used: ANOVA: One-way analysis of variance; DMEM: Dulbecco's modified minimum essential medium; FBS: Fetal bovine serum; HPLC: High-performance liquid chromatography; MCI: Methanol extract of the capitulum of Chrysanthemum indicum; NMR: Nuclear magnetic resonance; PPAR: Peroxisome proliferator-activated receptor.

The use of Lycii Fructus and Chrysanthemum Indicum in the Ophthalmology disease from literature and experimental studies review

Abstract: Objectives : To present rough guide for the proper use of Lycii Fructus and Chrysanthemum indicum on ophthalmic patients. Methods : Traditional medical classics(本草綱目, 銀海精微, 秘傳眼科龍木論, 東醫寶鑑) and recent studies for Lycii Fructus and Chrysanthemum indicum were reviewed. Results : The results are as follows. 1. According to traditional medical classics, the efficacy of Lycii Fructus and Chrysanthemum indicum is removing nebula(退翳) and improving acuity of vision(明目). 2. According to contemporary studies, Lycii Fructus has strong antioxidant, neuroprotective and anti-inflammatory effect. Chrysanthemum indicum has strong anti-inflammatory effect. Results and Conclusions : The Lycii Fructus is proper to macula or neuro-ophthalmology disease. Chrysanthemum indicum is proper to cornea or conjunctiva disease. Absolutely, It may be changed according to the individual condition. However, this may be a rough guide for usage in Korean medicine ophthalmology.

Method of preparing functional beverage

Abstract: The present invention relates to a production method of a functional beverage having a relaxing function and enhanced sleep-aid and cognitive functions, which comprises a step of mixing Schisandra chinensis fruit, Dimocarpus longan Loureiro, Liriope muscari, and Acorus gramineus to form a mixture; a step of extracting the mixture using a hot-water extraction or an ethanol extraction method; and a step of adding a distillate extract of Chrysanthemum indicum.

Formula of traditional Chinese medicine acne removing cream and preparation method thereof

Abstract: The invention relates to a formula of traditional Chinese medicine acne removing cream and a preparation method thereof. The formula of the traditional Chinese medicine acne removing cream comprises folium eriobotryae, cortex mori radicis, radix scutellariae, fructus gardeniae, chrysanthemum indicum, rhizoma imperatae, coptidis rhizoma, radix paeoniae rubra, raw pagodatree flower bud, honeysuckle,angelica sinensis, radix sophorae flavescentis and aloe. The traditional Chinese medicine acne removing cream is obtained after repeated trying and gradual improvement by the inventor, has a good curing effect on acnes, and has slight side effects.

Manufacturing method of ferment exract of chrysanthemum indicum

Abstract: The present invention relates to a method for producing a fermented Chrysanthemum indicum extract. More specifically, the present invention relates to a method for producing a fermented Chrysanthemum indicum extract, comprising the following steps: (S1) obtaining a fermented product by fermenting a culture solution composed of Chrysanthemum indicum, a base culture medium, and hydroxypropyl-beta-cyclodextrin with yeast; and (S2) producing an extract of the fermented product. According to the present invention, it is possible to minimize inhibition of microbial proliferation during a fermentation process.

Composition for Anti-Diabetes Using Compounds Isolated From Gynura procumbens et al

Abstract: The present invention discloses an anti-diabetic composition using compounds isolated from Chrysanthemum indicum from which the anti-diabetic activity is confirmed in a second diabetic animal model According to the present invention, it is possible to provide the anti-diabetic composition which can be produced into food or medicines

The complete plastid genome sequence of Chrysanthemum lucidum (Asteraceae): an endemic species of Ulleung Island of Korea.

Abstract: The characteristic of complete chloroplast (cp) genome sequence of Chrysanthemum lucidum, one of famous insular plant and an endemic to Ulleung Island of Korea, was firstly introduced in the present study. It was 150,985 bp and contained a large single copy region (82,786 bp) and a small single copy region (18,281 bp) which were separated by two inverted repeat regions (24,959 bp). In total, 131 genes were identified and they were consisted of 76 coding genes, eight rRNA genes, and 36 tRNA genes. Comparing to the previously reported Chrysanthemum indicum and C. x morifolium cp genomes, we found complete inversion of SSC region in this taxa. rpoC1 gene was pseudogenes due to 1 bp insertion of poly-A sequence in the 3′ of exon 2.

Comparative Analysis of the Flavor Compounds in Cultivated Chrysanthemum indicum L.

Abstract: This study investigated the chemical composition of four Korean cultivated Chrysanthemum indicum L. (Gamguk 1 ho, Gamguk 2 ho, Gamguk 3 ho, and Wonhyang) which are used in the food and fragrance industries to identify their volatile flavor compounds. These compounds were analyzed using headspace GC-MS from plant samples cultivated in the same region of Korea (Sancheong-gun, Gyeongsangnam-do). A total of 23 compounds were identified, eight of which were common across the four cultivars. The major flavor components in the three Gamguk plants were identified as 3-carene, camphene, β-phellandrene, eucalyptol and (+)-camphor. Eleven compounds, including (+)-camphor at 31.40%, were identified in Gamguk 1 ho. Gamguk 2 ho was found to contain 12 flavor compounds, predominant of which was camphene at 25.60%. Thirteen compounds including (+)-camphor (26.88%) were identified in Gamguk 3 ho, while 17 were detected in the Wonhyang cultivar, including trans-piperitol (47.33%), sabinene, and γ-terpinyl acetate. These results indicate differences in the type and ratio of functional volatile flavor ingredients in Chrysanthemum indicum L. cultivars which is highly valuable as material for fragrance product development.

Herbal extracts for treatment of herpesvirus infections

Abstract: Disclosed is a formulation comprising at least two of Rheum palmatum, Chrysanthemum indicum and Sanguisorba officinalis extracts or prevalent molecules therefrom and a pharmaceutically acceptable carrier. Further disclosed is a formulation comprising one or more of Rheum palmatum, Chrysanthemum indicum and Sanguisorba officinalis extracts, or prevalent molecules therefrom in combination with acyclovir and a pharmaceutically acceptable carrier. Disclosed also is a method for treating herpesvirus infections by administering a formulation comprising extracts of Rheum palmatum, Chrysanthemum indicum or Sanguisorba officinalis extracts or prevalent molecules therefrom with or without an antiviral agent, such as acyclovir and a pharmaceutically acceptable carrier.

Self-made chrysanthemum indicum bath lotion

Abstract: The invention relates to self-made chrysanthemum indicum bath lotion. The self-made chrysanthemum indicum bath lotion comprises, by weight, 30-50 parts of chrysanthemum indicum, 20-40 parts of honeysuckle, 5-10 parts of glycerin, 5-10 parts of honey, 80-100 parts of soap base and an appropriate amount of purified water. The self-made chrysanthemum indicum bath lotion can make the skin white, smooth and tender and also has effects of dispelling wind and clearing heat and calming the liver and improving eyesight.

Health-preserving tea beverage with effects of reducing weight and lowering blood pressure

Abstract: The invention relates to a health-preserving tea beverage with effects of reducing weight and lowering blood pressure. The health-preserving tea beverage with effects of reducing weight and lowering blood pressure consists of the following raw materials: 10-15 parts of lethariella cladonioides, 10-15 parts of notoginseng flower, 5-10 parts of ginseng flower, 10-15 parts of chrysanthemum indicum buds, 5-10 parts of dried hawthorn, 3-5 parts of stevia rebaudiana, 15-20 parts of honey, 0.05-0.1 part of pectin, 0.01-0.02 part of ethyl maltol, 0.02-0.03 part of butyl hydroxy anisd, 0.01-0.02 part of vitamin and 1000-1200 parts of drinking water. The health-preserving tea beverage with effects of reducing weight and lowering blood pressure has the beneficial effects that health-preserving effects of clearing away heat and toxic materials, reducing weight and lowering blood pressure and the like are achieved.

Patch composition for halt of breast-feeding and suppression of congestion in mothers breast

Abstract: An object of the present invention is to provide a patch composition for inhibiting breast congestion and promoting delectation. The present invention relates to a patch composition comprising a pharmaceutical composition comprising Chrysanthemum indicum, Lycium chinense, Malva verticillata, Zingiber officinale, Plantago asiatica and Rumex crispus.

Preparation method of chrysanthemum indicum polysaccharide plant disease-resistant inducer

Abstract: The invention discloses a preparation method of a chrysanthemum indicum polysaccharide plant disease-resistant inducer. The preparation method comprises the following specific steps: drying chrysanthemum indicum, carrying out coarse grinding, adding a grinding aid, and carrying out superfine grinding to obtain chrysanthemum indicum powder; adding water and 8-chlorotheophylline into the chrysanthemum indicum powder, carrying out ultrasonic extraction, carrying out suction filteration, concentrating the filtrate, carrying out alcohol precipitation, carrying out centrifugation, collecting the precipitate, and carrying out freeze drying to obtain a chrysanthemum indicum polysaccharide; supplementing the chrysanthemum indicum polysaccharide with a common pharmaceutical excipient, and preparingthe obtained substance into agent forms such as water aqua, powder and the like to obtain the disease-resistant inducer. The preparation method has the beneficial effects that the preparation method of the disease-resistant inducer is low in production cost and low in energy consumption, can improve the extraction efficiency and extraction rate of the chrysanthemum indicum polysaccharide, and canimprove the scavenging effect of the chrysanthemum indicum polysaccharide on different free radicals, and the prepared disease-resistant inducer can improve plant resistance and promote plant growth.

Skin-whitening skin-care composition containing chrysanthemum indicum cell extract, and preparation method thereof

Abstract: The invention discloses a skin-whitening skin-care composition containing a chrysanthemum indicum cell extract, and a preparation method thereof. The skin-care composition comprises the following components in parts by weight: 0.25 to 1.75 parts of a chrysanthemum indicum cell extract, 0.3 to 1.5 parts of fibroin, 2.0 to 6.0 parts of a liquorice root extract, 1.0 to 4.0 parts of a lotus flower extract, 0.5 to 2.5 parts of a peach flower extract, 5.0 to 8.0 parts of jojoba oil, 2.0 to 5.0 parts of a wetting agent, 1.5 to 5.0 parts of an emulsifier, 0.25 to 1.25 parts of a thickening agent and 40 to 60 parts of deionized water. The skin-whitening skin-care composition has a good inhibition effect on tyrosinase, has a good skin-whitening effect, contains the active components which are not liable to oxidation and deactivation, has high permeability on skin, can penetrate into the deep skin and is favorable for absorption of active components.

Medicinal herbal extract

Abstract: An object of the present invention is to provide a method for manufacturing a medicinal crop extract having anti-stress effect. The method for manufacturing a medicinal crop extract having anti-stress effect according to the present invention comprises the steps of: mixing 1 part by weight of Chrysanthemum indicum, 0.1-10 parts by weight of Cyperus rotundus, 0.1-10 parts by weight of Aucklandia lappa, 0.1-5 parts by weight of Syzygium aromaticum based on the weight of dried herbs; and extracting the mixture by any one distillation method selected from water distillation, steam distillation and water-steam distillation.

Antioxidant skincare product containing chrysanthemum indicum cell extracts and preparation method thereof

Abstract: The invention discloses an antioxidant skincare product containing chrysanthemum indicum cell extracts and a preparation method thereof The antioxidant skincare product is prepared from the followingraw materials by weight percent: 040%-280% of chrysanthemum indicum cell extracts, 025%-250% of myrtle fruit extracts, 100%-320% of vine tea extracts, 030%-160% of maize germ extracts, 300%-1200% of hydrogenated coconut oil, 100%-500% of surfactant, 300%-900% of wetting agent and the balance of deionized water The skincare product disclosed by the invention has an excellent synergetic enhancement effect for scavenging free radicals and resisting against oxidization and is mild and non-irritant to the skin