Showing papers on "Chrysanthemum indicum published in 2020"

Chrysanthemum indicum and Chrysanthemum morifolium: Chemical Composition of Their Essential Oils and Their Potential Use as Natural Preservatives with Antimicrobial and Antioxidant Activities.

Abstract: The composition of essential oils of Chrysanthemum indicum and C. morifolium were comparatively studied using both Gas Chromatography/Flame ionization Detector (GC/FID) and Gas Chromatography/Mass spectrometry (GC/MS) analyses. The antiviral activity was determined using a plaque reduction assay against three common viruses namely, herpes simplex type-1 (HSV-1), hepatitis A (HAV) and vesicular stomatitis virus (VSV). The antimicrobial activity was assessed using agar diffusion and microdilution methods and the minimum inhibitory concentration (MIC) values were determined. In addition, the anti-mycobacterial evaluation was carried out using the Alamar blue assay and the effect against Helicobacter pylori was investigated. The anti-trypanosomal activity was evaluated using the resazurin method. GC investigations revealed that camphor is the major constituent of both oils accounting for 36.69 and 14.56% in the essential oils from C. indicum and C. morifolium, respectively. C. indicum was biologically more active in all experiments; it exhibited a notable antitrypanosomal activity with an IC50 value equals 45.89 μg/mL and a notable antimicrobial activity versus Streptococcus agalactiae with a MIC value of 62.5 μg/mL. It also inhibited the replication of VSV with an IC50 value of 3.14 μg/mL. Both oils revealed antioxidant potential with IC50 values of 2.21 and 2.59 mg/mL for C. indicum and C. morifolium, respectively. This study provides evidence beyond the traditional use of both Chrysanthemum indicum and C. morifolium as anti-infective agents. Thus they could be used as spices in food and can be incorporated in different food products and pharmaceutical preparations as natural preservatives possessing antioxidant potential.

Chrysanthemum indicum L.: A Comprehensive Review of its Botany, Phytochemistry and Pharmacology.

Abstract: Chrysanthemum indicum L. (C. indicum L.), a member of the Compositae family, is a perennial plant that has been used as a traditional medicine for more than 2000 years in China and is widely used for the treatment of Pemphigus, swelling, pain, and scrofula. To date, more than 190 chemical constituents have been isolated and identified from this plant, including flavonoids, terpenoids, phenylpropanoids, and phenolic acids. Numerous modern studies have shown that extracts or monomeric compounds from C. indicum L. have several pharmacological activities, such as anti-inflammatory anti-oxidation, antipathogenic microorganism, anticancer, immune regulation, and hepatoprotective effects. However, resource availability, the research on the mechanism, and quality control are still insufficient, which deserves further efforts. In this paper, the advances in botany, phytochemistry, and pharmacology of C. indicum L were reviewed. We hope that this review can provide important information for traditional Chinese medicine, phytochemistry, synthetic and medicinal chemistry researchers for making full use of C. indicum L. resource.

Small-scale alpine topography at low latitudes and high altitudes: refuge areas of the genus Chrysanthemum and its allies

Abstract: Cultivated chrysanthemum (Chrysanthemum morifolium Ramat.) is an economically important ornamental plant species grown worldwide. However, the origin of the genus Chrysanthemum remains unclear. This study was conducted in the Hengduan Mountains, Yunnan Province. We took advantage of a special geographic region where the southernmost species of Ajania and the highest altitude population of Chrysanthemum indicum coexist to investigate their evolutionary origins. Diversity analysis of 9 populations of 5 species that came from 3 genera was carried out based on morphological traits and SRAP markers. Furthermore, topographical and ecological analyses and surveys of the vegetation communities in the plots were carried out for correlation analysis, and past data were used to reconstruct the ancient topography and vegetation to estimate the migration path and divergence time. We found that Chrysanthemum and Ajania were closely related based on the smooth transition states among marginal female florets and their common pollination system. The genetic relationship between Phaeostigma and Chrysanthemum was relatively distant, and Ajania was between them. Low light intensity and relatively humid habitats may be driving the elongation and evolution of marginal female florets. We found that Chrysanthemum and related genera were largely restricted to stony topographies at an altitude of ~3000 m.a.s.l. and in specialized alpine coniferous (Pinus) and broad-leaved (Quercus) mixed forest marginal communities. These stony topographies have become ecological islands of refuge for these species in the current interglacial period. The Hengduan Mountains play a key role in the evolution, divergence, and survival of Chrysanthemum and its allies.

Using profitable chrysanthemums for phytoremediation of Cd- and Zn-contaminated soils in the suburb of Shanghai

Abstract: We studied the profitable phytoremediation method with commercial chrysanthemum (Chrysanthemum indicum L.) in order to remediate the soils contaminated with heavy metals and generate economy income from the contaminated sites. A field experiment was carried out to remediate the contaminated soil through growing the commercial chrysanthemum plants in a farmland polluted with heavy metals of Cd and Zn due to application of creek sediments in the western suburb of Shanghai, Southeast China, since June 2013. After the consecutive 3 years of phytoremediation, Cd and Zn contents in the soil were reduced by 78.1% and 28.4%, respectively. We also found that the rice grain growing on the 3-year phytoremediated soil met the requirements of dietary safety, so did the vegetable growing on the 5-year phytoremediated soil. Growing chrysanthemum plants as a method of phytoremediation can not only remove a large amount of toxic heavy metals from the contaminated soil but also be highly profitable from the sales of chrysanthemum flowers.

Effect of Ethyl Methanesulfonate on Induced Morphological Variation in M3 Generation of Chrysanthemum indicum var. aromaticum

Abstract: Mutation breeding is considered to be economic and efficient in plant improvement, and the use of chemical mutagens such as ethyl methanesulfonate (EMS) can potentially address plant breeding challenges. The aim of this study was to induce morphological mutants in C. indicum var. aromaticum using EMS treatments with different doses, and to analyze the morphological and physiological traits of obtained mutants in expectation of finding favorable mutants. Results revealed significant effects of EMS doses on seed germination. The sample germination rate significantly decreased with increasing of EMS doses. The obtained morphological mutants were two viable types, containing leaf and stem mutants. Overall leaf size was significantly larger as a result of EMS treatments. And the height of mutant plants was significantly higher. Anatomical characteristics exhibited changes in both leaves and stems of the mutant plants. The puncture strength of the bent stem from the mutant plants was low, with weak penetration resistance. The total lignin and cellulose contents of mutant plants stem decreased significantly as a result of the EMS treatments. These results demonstrate the efficiency of EMS to induce mutations in C. indicum var. aromaticum, and this method can be useful in the future to assist breeding of this plant.

Molecular and morphological discrimination of Chrysanthemum indicum using allele-specific PCR and T-shaped trichome

Abstract: Chrysanthemum indicum L. is a traditional oriental medicinal herb prepared as a tea from flowers that have been used in China and South Korea since ancient times. It has a long history in the treatment of hypertension, inflammation, and respiratory diseases. Among Chrysanthemum species, C. indicum has more active chemical components as well as better therapeutic effects, and C. indicum is mostly used for medicinal purposes in South Korea. However, the usage of C. indicum has become problematic over the years due to the abundance of adulterated Chrysanthemum and confusion with morphologically related species such as C. morifolium, C. boreale, and Aster spathulifolius. Thus, here we developed a method for molecular authentication using chloroplast universal region rpoC2 and morphological authentication based on T-shaped trichomes of the adaxial leaf surface. By using a species-specific primer derived from the rpoC2 region, we established a multiplex allele-specific PCR for the discrimination of C. indicum. Amplicons of 675 bp for C. indicum and 1026 bp for other Chrysanthemum species were produced using both rpoC2-specific and common primers. These primers can be used to analyze dried samples of Chrysanthemum. Morphological discrimination was performed using T-shaped trichomes present only on the adaxial leaf surface of C. indicum species, and then molecular markers were utilized to authenticate C. indicum products from adulterant samples available in the market. Our results indicate that these molecular markers in combination with morphological differentiation can serve as an effective tool for identifying C. indicum.

New diploid populations of Chrysanthemum indicum L. (Asteraceae) from Korea

Abstract: Chrysanthemum indicum (Asteraceae) is a perennial plant belonging to the genus Chrysanthemum. The basic chromosome number of Chrysanthemum sensu stricto is х = 9, and it consists of a series of polyploids ranging from diploid to decaploid. However, C. indicum, which occurs in Korea, is known to consist of only tetraploids, except for two diploid populations that are sympatric with C. zawadskii and C. boreale. During the collection of plant materials as part of a study to ascertain the diversity of Chrysanthemum in Korea, we found new diploid populations (2n = 18) of C. indicum in the southern region of Korea and describe them here in detail.

Validation of Natural Cosmetic Resources Containing EtOH Extracts of Chrysanthemum indicum and Cymbopogon cirtratus by the Simultaneous Analysis of Their Marker Compounds Using High Performance Liquid Chromatography-Diode Array Detector

Abstract: Purpose: Recently, the demand for natural extracts and naturally derived ingredients from core raw materials has increased in the cosmetic industry, but their manufacturing and quality control standards are not sufficiently established. This study aims to standardize the compositions of marker compounds from natural cosmetics using ethanol extracts of Chrysanthemum indicum (CI) and Cymbopogon cirtratus (CC). The method is then validated. Methods: In liquid chromatography (LC)/mass spectrometry, astragalin and p -coumaric acid were determined as the marker compounds for quantitative analysis. The performances (linearity, accuracy, and precision) of these two marker components were simultaneously analyzed in a mixture of both natural products using high-performance liquid chromatography with a diode array detector (HPLC-DAD). Results: The marker compounds of CI and CC in the extracts were determined as astragalin and p -coumaric acid, respectively. The detected marker compounds from the CI and CC showed significant linearity (R2≥0.9999). The astragalin and p -coumaric acid markers achieved limits of detection of 1.20 and 0.07 ng/mL, respectively, and limits of quantification of 3.59 and 0.22 ng/mL, respectively. The marker concentrations were 0.24 μg/mL in CI and 1.55 μg/mL in CC. Conclusion: This study suggests that the developed method may be an important basic data for the manufacturing and quality control of natural cosmetic.

An assessment of the interaction for three Chrysanthemum indicum flavonoids and α-amylase by surface plasmon resonance.

Abstract: This study evaluated the interaction of Chrysanthemum indicum (CI) flavonoids (luteolin, acacetin, and buddleoside) with α-amylase. Surface plasmon resonance (SPR) assay showed their equilibrium dissociation constants (KD ) are 1.9695 ± 0.12, 2.9240 ± 0.20, and 3.2966 ± 0.08 mM at pH 6.0, respectively. Furthermore, their binding affinities were influenced by KCl, MgCl2, and CaCl2. Enzymatic kinetic studies revealed that three flavonoids exhibited noncompetitive α-amylase inhibitory activity. The inhibitory sequence is luteolin > acacetin > buddleoside, which was in accordance with the results of binding affinity from SPR. 1,1-diphenyl-2-picryl hydrazyl radical assay demonstrated that antioxidant activities of three flavonoids were inhibited significantly with α-amylase. Meanwhile, the study reveals that hydroxyl on C'-4, C'-5, and C-7 of flavonoids play an important role on the interaction of three flavonoids with α-amylase. Also, SPR could be used as sensor for rapid screening inhibitors of α-amylase and provide useful information for the application of C. indicum flavonoids in food and pharmaceutical area.

Inhibitory Effect of Lactic Acid Bacteria-fermented Chrysanthemum indicum L. on Adipocyte Differentiation through Hedgehog Signaling

Abstract: 1 Department of Culinary Arts & Hotel Food Service-Major in Culinary Arts, Yeonsung University, Yanghwa-ro 37beon-gil 34, Manan-gu, Anyang-si, Gyeonggi-do 14011, Korea L.FOUNDER INC., 406, Business Incubation Seoul National University of Science & Technology 232, Gongneung-ro, Nowon-gu, Seoul 01811, Korea Department of Biological Engineering, College of Engineering, Konkuk University, Neuundong-ro 120, Gwangjin-gu, Seoul 05029, Korea

ANTI-DIABETIC PROPERTY OF GREEN SYNTHESIZED ZINC-OXIDE NANOPARTICLES FROM LEAF EXTRACT OF Chrysanthemum indicum PLANT

Abstract: Diabetes mellitus gives a particular mortality and diabetes belonging tangles. Traditional medicines are applied in control of diabetes mellitus but are expensive and are not easily receivable and as well keep several side effects. Herbal plants used in diabetes mellitus treatment are considered to be inexpensive and easily acquirable. In recent studies nanoparticles of zinc oxide were estimated to check the anti-diabetic property. 36 rats of weight 150-200 gm and age 6-8 weeks were used. The study showed that zinc oxide nanoparticles showed considerably reduced blood glucose in diabetic rats. In inference, the nanoparticles of zinc oxide act as proponents’ anti-diabetic agent. Key-Words: Diabetic Mellitus, Diabetic Activity, Zinc Oxide Nanoparticles, Anti-diabetic Agent. © RASĀYAN. All rights reserved INTRODUCTION Diseases with the clinical features of diabetes have been recognized since antiquity. The Ebers papyrus, dating from 1500 BC depict a polyuric manifest that resembles diabetes. The word 'diabetes' was at first applied by Aretacus of Cappadocia in the second centenary AD. Diabetes is a deficit in the body's capability to modify glucose (sugar) to energy. Glucose is the main resource of energy for our body. When food assimilate it is exchanged in the form of fats, proteins or carbohydrates. Foods that affect blood sugar are called carbohydrates. Carbohydrates when digested change into glucose. Examplebread, pasta, potatoes, corn, fruits and milk products. Particular with transpose to the blood and is employed by cells for energy. In this way for glucose to be transpose from blood into the cells, the hormone-insulin is expected. One of the essential components in our body is Insulin which is a hormone that go through by means of pancreas, the work of pancreas is to promote glucose which enter in the cells and give energy to the body. The prevalence of diabetes mellitus is rising and it is now the seventh salient reason for death in the USA. In the present time, the current rate of diabetics increased (6% per year), the number of diabetic patients is increasing day by day and it will be double in coming 15years. Epidemiol logically, diabetes mellitus has been connected to the western lifestyle and is unusual in upbringing consuming a more “primitive” diet. All modus of diabetes are on the outclass, type 2 diabetes in exclusive the people who are suffering from diabetes will increase by 55% by 2035. EXPERIMENTAL Collection of Samples The leaf of Chrysanthemum indicum, gathered from the Department of Horticulture, SHUATS, Prayagraj. Place of Work The present work was carried out in the Department of Pharmacy, United Institute of Pharmacy, UCER, Prayagraj. Vol. 13 | No. 1 |570 573| January March | 2020 571 ZINC-OXIDE NANOPARTICLES Neha Silas and Reena S. Lawrence Drug, Chemicals and Instruments Streptozotocin (STZ) chemical name 2 deosil-2-(3-methyl-3-nitroso urea), citrate buffer, metformin, zinc oxide nanoparticles. Test-tubes, Capillary Tubes, Blood Glucose Monitoring System. Preparation of Zinc Oxide Nanoparticles The zinc oxide nanoparticles synthesized using the Chrysanthemum indicum plant extract. 1ml of Chrysanthemum indicum extract will be taken and boiled to 60-80C using a stirrer-heater. 1mM Zinc Nitrate solution (9ml) is added to the solution as the temperature reaches 60C, the mixture is then boiled till it turn into a paleyellow coloured solution. This indicates the formation of ZnO nanoparticles. Fig.-1: C.indicum Leaf Extract Fig.-2: Formation of ZnO NPs Animals involved in the Study Young male Wister rats which were 7-8 weeks old weighing 150-200 gm was taken from inbred animal house of C.D.R.I, Lucknow. The animals were kept in polypropylene cages in the favorable environmental conditions of 12 h light and 12 h dark at room temperature. The rats were given with the standard laboratory pellet diet and water. At least 15 days before the experiment, rats were made habitual to the laboratory condition and prepare for the experiment. To maintain the laboratory condition, they were kept in a well ventilated animal house. The experimental protocol was approved by the Institutional Animal Ethical Committee (IAEC) animal and the care of the laboratory was taken as per the CPCSEA regulation (Reg. No1451/PO/Re/S/11/CPCSEA Dated 06/05/2018). Determination of Oral Acute Toxicity Study As per the Organization for Economic Co-operation and Development (OECD), Guideline 423 A, the lethal medium dose (LD50) was performed in rats to start the experiment. Preparation of Extract Dose Weighed quantity of zinc oxide nanoparticles was suspended in water and delivered orally to experimental animals. Suspension of extract was prepared freshly. The extract was administered at a dose of 200-400 mg/kg of body weight. EXPERIMENTAL The solution of STZ (50 mg/kg of body weight) in 0.1 M citrate buffer was freshly injected intraperitoneally in a volume of 1 ml/kg. After injecting STZ to the animals they show hyperglycemia within 2 days. By measuring the fasting blood glucose level after 48 hours after the injection of STZ, diabetes was confirmed. In STZ rats, the rats whose blood glucose level over 200 mg/dl was indicated as diabetic and grouped in different groups for further experiments. Research Design and Procedure Five groups of rats were used to study the effect of Chrysanthemum indium nanoparticles. Each group having six rats. Group I: The control rats, administered interaperitoneally or orally with normal saline solution. Vol. 13 | No. 1 |570 573| January March | 2020 572 ZINC-OXIDE NANOPARTICLES Neha Silas and Reena S. Lawrence Group II: Diabetic control rats, administered interaperitoneally or orally with normal saline solution. Group III: Diabetic rats, administered interaperitoneally or orally with extract 200 mg/kg body weight. Group IV: Diabetic rats, administered interaperitoneally or orally with extract at 400 mg/kg body weight. Group V: Diabetic rats, administered interaperitoneally or orally with leaf extract of Chrysanthemum Indicum. Group VI: Diabetic rats, administered interaperitoneally or orally with the standard drug metformin at 100 mg/kg body weight. Anti-hyperglycemic Activity All the rats were kept inside the cage for 3 days to adapt to the experimental condition, after which they have fasted overnight. Diabetes was determined in rats by intraperitoneal injection of STZ dose of 50 mg/kg which was dissolved in normal saline. All the animals were given food and water after the STZ treatment. 2 days after STZ injection, blood glucose level was checked and used as parameter to match pair of diabetic rats with an identical level of intensity only the rats whose fasting blood glucose level is higher than 200mg/dl were consider to be diabetic and used in experiment. In normal rats, the mean blood concentration of glucose was 86mg/dl. Animals were separated into 6 different groups of 6 rats i.e I to VI. Normal control rats were named as Group I. two days after STZ injection, the treatment was started. By using oral gavages tube, the drugs were administered daily, orally for 3 weeks. Blood samples were collected from eyesight for the measurement of blood glucose levels. The blood glucose level was checked by glucometer (one-touch). RESULTS AND DISCUSSION The effect of oral administration of zinc oxide nanoparticles of Chrysanthemum Indicum on blood glucose level of animals in 21 days is depicted in Table-1. Fig.-3: Graph Showing Blood Glucose Levels in STZ-induced Diabetic Rats in 21 days

Antidepressant Activity of Flower Extract of Chrysanthemum indicum L. in Experimental Animal

Abstract: Depression is a common symptom in today's scenario. The Ethanolic extract of Chrysanthemum indicum Linn (EECI) contains flavanoids compound which has shown therapeutic potential in neurological diseases. The study was undertaken to evaluate the antidepressant activity of EECI using the Despair Swim Test (DST) & Tail Suspension Test (TST). The Swiss Albino mice weighing about 20-25 gm were used. The animals were divided into 4 groups, each group comprising of 6 animals,(n=6). Group I was controlling received Distilled Water (10ml/kg per oral), Group II Standard Imipramine HCl (10gm/kg per oral) and Group III & IV Test group, receives EECI (250& 500 mg/kg per oral respectively). All drugs were administered for 10 days. The results were analyzed using one way ANOVA followed by Dunnett test, p<0.05 was considered as significant. The effect of EECI on immobility periods of mice was assessed in DST & TST. The effect of EECI was compared with that of control. The effect of 500mg/kg showed a significant reduction in immobility time of mice in both DST & TST. The present study suggests that possible antidepressant activity of EECI in mice on the 500mg/kg drug administration is more than 250mg/kg.

Natural deodorant composition using polyphenol

Abstract: Disclosed is a natural deodorant composition using polyphenol components. The natural deodorant composition using the polyphenol components of the present invention is characterized by comprising Korean angelica root, green tea, sodium hydrogen carbonate, Chrysanthemum indicum, cinnamon, Illicium verum and purified water. The natural deodorant composition is harmless to a human body by using natural substances.

Composition for inhibiting adipogenesis comprising Chrysanthemum indicum fermented by lactic acid bacteria

Abstract: The present invention relates to a composition for inhibiting adipogenesis or reducing obesity, which is excellent in the inhibitory effect of adipogenesis through regulation of the Hedgehog signaling pathway of Chrysanthemum indicum fermented by using lactic acid bacteria.

Composition comprising natural complex extract for protecting liver and relieving hangover

Abstract: The present invention relates to a composition for protecting liver functions and relieving hangover, containing a complex natural extract as an active ingredient More specifically, the present invention relates to a composition for protecting liver functions and relieving hangover, which exhibits an excellent hangover relieving functions and liver protection effects by containing, as an active ingredient, a complex natural extract composed of Oenanthe javanica, bean sprouts, Artemisia capillaris Thunberg, Hovenia dulcis Thunb fruits, quince, pueraria, licorice, Chrysanthemum indicum and unripe Rubus coreanus fruits in a specific mixing ratio

Efficacy of various fungicides and nano-chemicals against Fusarium wilt of Chrysanthemum

Abstract: Chrysanthemum flower (Chrysanthemum indicum Ramat) is popularly designated as “Queen of the east”, or autumn queen (as its bloom in November-December) (Shibata, 2008; Teixeira et al., 2013). Fusarium oxysporum f. sp. chrysanthemi (Foc) causing vascular wilt is one of the most devastating pathogens attacking Chrysanthemum. This pathogen bears a great ability to attack all the growth stages, ranging from nursery to flowering stages (Pinto et al., 2010). In this experiment, an attempt was made to manage this theat (Foc) by means of three systemic (Propiconazole, Hexaconazole, Vitavax) and two non-systemic fungicides (Mancozeb, Thiram) at four different concentrations (100, 200, 300 and 400ppm), both in lab (in-vitro) and in pot conditions. And the observations reflected that Mancozeb was the most effective in terms of radial growth inhibition and disease severity, followed by Propiconazole for all the tested fungicides at all the concentrations in both experiments. A significant effectiveness over control was also noted for Hexaconazole and Vitavax, but only at their higher concentrations in poison food technique. While in pot experiments, Hexaconazole and Vitavax produced quite similar effects. Among all tested fungicides, Thiram stands to be least effective at all the concentrations. An attempt was made to compare the effectiveness of four selected nano-chemicals namely, Copper, Molybdenum, Cobalt and Magnesium, over above mentioned traditional chemicals at the same concentration, both in vitro and in pot condition. Interestingly, a superiority of traditional fungicides was recorded in both in vitro and pot experiments. Among all the nano-particles under evaluation Cu NP was the most effective, followed by Molybdenum. Magnesium nanoparticles were found to be the least effective among all the tested nanoparticles. It was noteworthy that all the nanoparticles were effective, only at there higher concentrations.

Anti-inflammatory composition containing mixed extracts of fermented Gentianae scabrae Radix Chrysanthemum indicum and Mentha piperascens

Abstract: The present invention relates to a pharmaceutical, health functional food, health food, and cosmetic composition for anti-inflammation containing a fermented Gentiana scabra extract, a Chrysanthemum morifolium extract, and a Mentha Canadensis extract. The composition according to the present invention contains a mixed extract of one or more extracts selected from a group consisting of the fermented Gentiana scabra extract, the Chrysanthemum morifolium extract, and the Mentha Canadensis extract, thereby having an effect of significantly improving an anti-inflammatory effect. In addition, the composition does not affect a cell survival rate to have no side effects, thereby being able to be usefully used for the natural pharmaceutical, health functional food, health food, and cosmetic composition for anti-inflammation.

Method for preparing chrisanthemum indicum extract containing increased content of aglycon by enzymes and composition for preventing or treating obesity made thereby

Abstract: The present invention relates to a method for preparing an enzyme-treated extract of Chrysanthemum indicum having an increased content of aglycone, and a composition for preventing or treating obesity prepared thereby. According to the present invention, the enzyme-treated extract of Chrysanthemum indicum prepared by complex enzyme treatment maximizes an extraction yield and the content of aglycone, and the extract exhibits an anti-obesity effect by inhibiting the differentiation of adipocytes and the production of triglycerides, and activating AMPK which inhibits expression factors related to obesity. Therefore, the extract may be usefully utilized as a pharmaceutical composition for preventing or treating obesity, or a health food composition for preventing or ameliorating obesity.

Phytoremediation method suitable for cadmium-contaminated soil in northern areas of China

Abstract: The invention discloses a phytoremediation method suitable for cadmium-contaminated soil in northern areas of China. The method comprises the following steps: sowing Chrysanthemum indicum seeds in thecadmium-contaminated soil in March of the same year, wherein the use amount of the seeds is 0.1 g/m , and the sowing depth is 5 +/-1 cm; timely removing weeds and irrigating plants in the plant growth period to keep good moisture content of soil; performing harvesting in the middle ten days of September, cutting off the overground parts of the plants with plant roots left in the soil; and growing new Chrysanthemum indicum seedlings from the roots left in soil in March of the second year. The method has a high remediation efficiency, tests show that the biological enrichment coefficient of the Chrysanthemum indicum to soil cadmium reaches up to 7.76, and the remediation method is simple, is easy to master, and has a better remediation effect on the northern cadmium-contaminated soil thanexisting methods.