Topic
Circular dichroism
About: Circular dichroism is a research topic. Over the lifetime, 24086 publications have been published within this topic receiving 732164 citations. The topic is also known as: CD.
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TL;DR: A self-consistent procedure for estimating the secondary structure content from circular dichroism spectra of proteins, which finds the Kabsch and Sander protein crystal structure analysis to be most suitable for the prediction method.
1,009 citations
TL;DR: Fluorescence, absorption, mass, and NMR spectra all indicate that multiple species are present, but that their creation is both nucleotide- and time-dependent, and that the nanoclusters are associated with the chiral ss-DNA template.
Abstract: The high affinity of Ag+ for DNA bases has enabled creation of short oligonucleotide-encapsulated Ag nanoclusters without formation of large nanoparticles. Time-dependent formation of cluster sizes ranging from Ag1 to Ag4/oligonucleotide were observed with strong, characteristic electronic transitions between 400 and 600 nm. The slow nanocluster formation kinetics enables observation of specific aqueous nanocluster absorptions that evolve over a period of 12 h. Induced circular dichroism bands confirm that the nanoclusters are associated with the chiral ss-DNA template. Fluorescence, absorption, mass, and NMR spectra all indicate that multiple species are present, but that their creation is both nucleotide- and time-dependent.
980 citations
TL;DR: Z-DNA in Other Naturally Occurring Sequences, e.g. d(CA/GT}n, Drosophilia and Chironomus, and Other Species : 831.
Abstract: CONTENTS PERSPECTIVE AND SUMMARY 792 INTRODUCTION 793 MOLECULAR STRUCTURE OF Z-DNA 795 METHODS FOR DETECTING Z-ONA 803 Physical-Chemical Methods 804 Use of Specific Anti-Z-DN A Antibodies 806 PHYSICAL-CHEMICAL STUDIES OF Z-DNA 809 Ultraviolet Circular Dichroism and Absorbance 809 Raman Spectra 810 Nuclear Magnetic Resonance Studies 8 1 1 Other Methods 813 Theoretical Analyses: Energetics and Mechanics 813 CHEMICAL FACTORS INFLUENCING THE EQUILIBRIUM BETWEEN BAND Z-DNA 816 Covalent DN A Modifications 816 Ions, Solvents, and Small Molecules 818 Cytosine Methylation in CG Sequences Stabilizes Z-DNA 819 NEGATIVE SUPERCOILING STABILIZES Z-DNA 821 Topological Constraints in DN A 821 Form V DNA 825 PROTEINS THAT BIND TO Z-DNA 826 NUCLEOTIDE SEQUENCES THAT FAVOR Z-DNA FORMATION 828 Polymers and Repetitive Sequences, e.g. d(CA/GT}n 828 Z-DNA in Other Naturally Occurring Sequences 829 CYTOLOGICAL STUDIES OF Z -DNA IN CHROMOSOMES 830 Drosophilia and Chironomus 830 Other Species : 831
977 citations
967 citations
TL;DR: Circular dichroism is a spectroscopic method which depends on the fact that certain molecules interact differently with right and left circularly polarized light and can provide information about the secondary structure of proteins and nucleic acids and about the binding of ligands to these types of macromolecule.
Abstract: Publisher Summary Circular dichroism (CD) is a spectroscopic method which depends on the fact that certain molecules interact differently with right and left circularly polarized light. Circularly polarized light is chiral—that is, it occurs in two nonsuperimposable forms that are mirror images of one another. To discriminate between the two chiral forms of light, a molecule must be chiral, including the vast majority of biological molecules. A method that can discern the subtle differences between non superimposable mirror image molecules (enantiomers) must be highly sensitive to the three-dimensional features of molecules—that is, to conformation. Binding of ligands or protein-protein and protein–DNA interactions can also alter the circular dichroism spectrum of the protein and/or nucleic acid. These changes in CD can be used to determine equilibrium constants, and they can also provide evidence for conformational changes. Thus, CD can provide information about the secondary structure of proteins and nucleic acids and about the binding of ligands to these types of macromolecule.
941 citations