Showing papers on "Color reaction published in 1979"
TL;DR: A new spectrophotometric method for the determination of formaldehyde is described, based on a colour reaction with oxalyidihydrazide and copper(II); the optimum reaction conditions, and other analytical parameters such as interferences, Beer's law, sensitivity, collection efficiency, etc. have been studied.
18 citations
TL;DR: An improved method is now proposed which is excellent on the ground of convenience and reproducibility; same readings were obtained with the tetraborate solution from various preparations which were stable at least for two years.
18 citations
TL;DR: The formation of hydroxylated anions of I (I) and the presence of equilibria between the isomers of I and I' were confirmed by nuclear magnetic resonance studies.
Abstract: The color observed in the Jaffe reaction is due to the formation of the isomers of the 1 : 1 σ-complex (I) of creatinine and picric acid The formation of hydroxylated anions of I (I') and the presence of equilibria between the isomers of I and I' were confirmed by nuclear magnetic resonance studies The phenomenon of the augmentation of the color intensity on neutralizing the alkaline reaction mixture of picric acid and creatinine can be explained in terms of shifts of the equilibria between the isomers of I and I' Three isomers of the 1 : 2 σ-complex are formed via the isomers of I and I' in an alkaline mixture of picric acid and creatinine, but not in the presence of a large excess of picric acid, as determined by high performance liquid chromatography
6 citations
TL;DR: Heterocyclic cation and aromatic anion are used in the chromatographic buffers for the analysis of monoamines and diamines on a sulfonated cation-exchange column using an amino acid analyzer, and have been successfully used for biological samples.
6 citations
Patent•
06 Dec 1979
TL;DR: In this paper, an antibiotic A-11079-B1b and its pharmaceutically acceptable salt are described. But the antibiotic is not suitable for use in the treatment of cancer.
Abstract: NEW MATERIAL:An antibiotic A-11079-B1b and its pharmaceutically acceptable salt Color white Melting point 216 degC; molecular weight 263; molecular formula C11H13N5O3; specific rotatory power [alpha] D-157 deg (C=045% H2O) Solubility soluble in water, DMSO, DMF, etc; insoluble in ethyl acetate, chloroform, etc Color reaction:KMnO4 decolorization, periodic acid color reaction: positive; FeCl3 reaction, ninhydrin reaction, etc: negative USE:Antifungal agents, carcinostatics, and intermediates for the synthesis of nucleic acid-type physiologically active substances PROCESS:Strains of the genus Ampullariella sp A 11079 (FERM-P No4494) are cultured in a solid or liquid medium at 25-30 degC at a neutral or weakly basic pH, and the objective substance is separated, collected, and purified by an adsorbent or ion exchange resin to give the antibiotic A-11079-B1b
6 citations
TL;DR: In this paper, a fungal enzyme, l-Iysine α-oxidase (EC 1.4.3), was used to determine l-lysine levels in serums from several animals without pretreatments.
Abstract: l-Lysine could be determined satisfactorily with a new fungal enzyme, l-Iysine α-oxidase (EC 1.4.3). The method consists of the oxidative deamination of l-Iysine with l-lysine α-oxidase and the spectrophotometric determination of one of the reaction products: α-keto-e-aminocaproate, its intramolecular dehydrated form, Δ1-piperideine-2-carboxylate or hydrogen peroxide. The method on the basis of the color reaction of hydrogen peroxide formed from l-lysine with 4-aminoantipyrine and phenol in the presence of peroxidase was most sensitive and simple. The method could be used for the direct assay of l-lysine levels in serums from several animals without pretreatments.
5 citations
TL;DR: A new colorimetric method for the serum enzyme assay was developed, using the color reaction of a ferrous salt complex and a primary aromatic amine, in good agreement with those of the p-nitroanilide method used to assay each enzyme.
4 citations
Patent•
09 Oct 1979
TL;DR: In this article, a small amount of eithanol is added to the crude extract, and insoluble substance is isolated by carrying out thin-layer chromatography three times three times.
Abstract: NEW MATERIAL:Heterocyclic phenol compound AOS Elemental analysis: C 6264, H 607, N 562, O 2568%; molecular weight: 284; mp: 1246-1254 degC; solubility: slightly soluble in water, insoluble in n-hexane and easily soluble in ether and ethanol; color reaction: positive to nitrous acid, Rhodamine B, DPPH or PDAB; shows brown color with PNA or PNA and sodium carbonate, and blue to green with 50% sulfuric acid; color; light brown USE:Natural antioxidant of fats and oils, food, confectionery, etc containing thereof, etc PROCESS:Defatted safflower seeds are extracted with a polar organic solvent, to obtain crude extract (the yield is about 2%) A small amount of eithanol is added to the crude extract, and insoluble substance is isolated The filtrate is cooled, centrifuged, and-subjected to liquid column chromatography Then, AOS is isolated by carrying out thin-layer chromatography three times
3 citations
Patent•
24 Mar 1979
TL;DR: P-725A as mentioned in this paper is an acidic oil-soluble colorless prismatic crystal, which decomposes at 165-170 deg c.C. and is shown to be soluble in methanol, tetrahydrofuran, etc.
Abstract: PURPOSE:Physiologically active substance P-725A. Characteristics thereof are as follows: Acidic oil-soluble colorless prismatic crystal. Melting point: decomposes at 165-170 deg.C. Elementary analysis: C 45.95, H 4.45, N 8.94, O 41.22. Molecular weight: 157. Molecular formula: C6H7NO4. Specific rotation: [alpha] D =+48.9 (C=1.0, methanol). Color reaction: positive to iodo reaction, ninhydrin reaction, etc.; negative to naphthoresorcinol-sulfuric acid reaction. Solubility: soluble in methanol, etc., easily soluble in tetrahydrofuran, etc. and slightly soluble in benzene, etc.UV absorption spetrum: for example, shows absorption maximum at 293nm (E 1%cm 758) in methano
1 citations
Patent•
17 May 1979
TL;DR: Insecticide and miticide effective to tetranychidae and noxious insects using FERM-P No.4084 is described in this article, and the desired compounds are collected from the filtrate of the culturing medium by fractionation using an adsorbent.
Abstract: NEW MATERIAL:Antibiotics C-8030B, C, D and E. characteristics thereof are as followa; specific rotation [alpha] D (water solution): B=8.0 deg., C=+12.5 deg., D=-10.8 deg., E=-9.1 deg.; molecular weight: B 600, C 800, D 600, E 700; solubility: all compounds are easily soluble in water and dimethyl sulfoxide, and slightly soluble in methanol, etc.; color reaction: all components are positive to periodic acid-benzidine reaction, etc., quasi-positive to Ehrlich reaction, and negative to phthalic acid-aniline reaction, etc.; C compound is unstable in a basic condition and stable in a neutral or acidic condition; the others are stable in a basic and an acidic conditions. USE:Insecticide and miticide effective to tetranychidae and noxious insects. PROCESS:Streptomyces species C-8030 (FERM-P No.4084) is cultured, and the desired compounds are collected from the filtrate of the culturing medium by fractionation using an adsorbent, etc.
1 citations
Patent•
22 Oct 1979
TL;DR: Antibiotic AB-102 showing the following physicochemical properties Specific rotation: [alpha] D0 (c=05, in DMSO); elemental snalysis: C 4944, H419, N 1796, S 795; mp: does not show clear melting point up to 300 degC; color reaction: negative to ninhydrin reaction and anthrone reaction, and positive to biuret reaction; solubility: easily soluble in CH3OH CHCl3 mixed solution and C2H5 OH CHCl 3 mixed soution, soluble in p
Abstract: NEW MATERIAL:Antibiotic AB-102 showing the following physicochemical properties Specific rotation: [alpha] D0 (c=05, in DMSO); elemental snalysis: C 4944%, H419%, N 1796%, S 795%; mp: does not show clear melting point up to 300 degC; color reaction: negative to ninhydrin reaction and anthrone reaction, and positive to biuret reaction; solubility: easily soluble in CH3OH CHCl3 mixed solution and C2H5 OH CHCl3 mixed soution, soluble in pyridine, concentrated hydrochloric acid, etc, slightly soluble in CHCl3, etc, and insoluble in CH3OH, etc; etc USE:Antibiotic Active against Gram-positive bacteria, particularly acid-fast bacteria such as resistant bacteria and tuberculous bacilli PROCESS:Actionplanes japanencis subspecies japanencis 221-2 (FERM-P NO4464), etc is cultured, and antibiotic AB-102 is separated from a cultured substance and purified
Patent•
12 May 1979
TL;DR: In this article, a wide variety of antibacterial preparations are described, such as Micromonospora sagamiencis SU-2 (FERM-P No.4230) and its nontoxic acid adduct salts.
Abstract: NEW MATERIAL:Antibiotic SU-2 and its nontoxic acid adduct salts. Characteristics thereof are as follows: basic white powder; elemental analysis: C48.38%, H8.72%, N11.83%; molecular weight: 450; molecular formula: C19H38N4O8; m.p.: 102- 112 deg.C; UV absorption spectrum does not show characteristic absorption peaks and only shows terminal absorptions in the region of 280-360nm; easily soluble in water, soluble in methanol, etc. and insoluble in chloroform, etc,; color reaction: positive to ninhydrin reaction, etc. and negative to Elson-Morgan reaction, etc. USE:A wide variety of antibacterial preparations. Effective also against bacteria of Proteus genus and Pseudomonas genus, and used in medicines and cleaning agents. PROCESS:Micromonospora sagamiencis SU-2 (FERM-P No.4230) is cultured, and the desired substance is collected from a culture product by an adsorption-desorption method with a cation-exchange resin, etc.
TL;DR: In this article, the use of a new reagent for the detection of nitrogen dioxide in air and water is reported, based on the reaction of the nitrite ion with catechol in an acetic acid medium and the resultant nitroso-compound is coupled with p‐aminophenol.
Abstract: The use of a new reagent for the detection of nitrogen dioxide in air and water is reported. The method is based on the reaction of the nitrite ion with catechol in an acetic acid medium and the resultant nitroso‐compound is coupled with p‐aminophenol. A violet colour indicates the presence of nitrite. The method can be used for the detection of oxides of nitrogen in air up to 0.8 ppm and in water up to 0.5 mg/litre.
Patent•
12 May 1979
TL;DR: In this article, the authors described the characteristics of the Staphylococcus SP No 216 (FERM-P No3773) under aerobic conditions, and the desired substance was purified and collected using ion exchange resins.
Abstract: NEW MATERIAL:Antibiotic No 216 substance Characteristics thereof are as follows: orange-yellow amorphous flaked having metallic luster; elemental analysis: C 3883%, H 522%, N 1391%, S 262%, Fe 468%; mp: above 300 degC; color reaction: negative to ninhydrin reaction, etc and quasi-positive to Molisch's reaction; stability of an aqueous solution: unstable in an acidic side and stable in a neutral or alkaline solution, dicomposed and deactivated by heating in a neutral solution at 100 degC for 60 min; solubility: easily soluble in water and dimethyl sulfoxide, slightly soluble in methanol and insoluble in ethanol, etc USE:Antibiotic PROCESS:Streptomyces SP No 216 (FERM-P No3773) is cultured under aerobic conditions From the filtrate obtained by removing the fungi from the cultured substance, the desired substance is purified and collected, in an example, using ion- exchange resins, etc