Showing papers on "Color reaction published in 1982"
Patent•
27 Dec 1982
TL;DR: In this article, the authors present a technique to obtain a repeatedly usable recording material capable of easily performing a color reaction and erasing through temperature change, wherein a composition comprised of an electron-donative color reaction organic compound, a phenolic OH containing organic compound or the like and an alcohol, an ester, a ketone, an ether, a thiol, a (di)sulfide and the like, exhibiting a hysteresis characteristic in a temperature range of not narrower than 3°C is applied onto the surface of the base to obtain the recording material
Abstract: PURPOSE: A repeatedly usable recording material capable of easily performing a color reaction and erasing through temperature change, wherein a composition comprised of an electron-donative color reaction organic compound, a phenolic OH containing organic compound or the like and an alcohol or the like is applied onto a base. CONSTITUTION: A coating composition comprising as a color reaction component a thermally decoloring material consisting of a uniform mixture of an electron- donative color reaction organic compound (e.g., crystal violet lactone), a compound selected from the group consisting of a phenolic OH containing compound (a metallic salt thereof), an aromatic carboxylic acid, a 2W5C aliphatic carboxylic acid ester, an acidic phosphoric acid ester (a metallic salt thereof) and 1,2,3- triazole (a derivative thereof) and a compound selected from the group consisting of an alcohol, an ester, a ketone, an ether, a thiol, a (di)sulfide and the like and exhibiting a hysteresis characteristic in a temperature range of not narrower than 3°C is applied onto the surface of the base to obtain the recording material. COPYRIGHT: (C)1984,JPO&Japio
9 citations
TL;DR: Recoveries of nitrate from vegetable products were satisfactory and the standard deviation for the whole procedure was 1.41% for the 42 determinations; the detection limit of the method is 1.3 p.p.m. for nitratenitrogen.
Abstract: A procedure is described for the determination of nitrate in vegetable products, based on the quantitative reaction of nitrate and 2-sec-butylphenol in sulphuric acid (5 + 7), and the subsequent extraction and measurement of the yellow complex formed in alkaline medium. The colour reaction is sensitive and stable and absorbances measured at 418 nm obey Beer's law for concentrations of nitrate-nitrogen between 0.13 and 2.50 µg ml–1. Various possible interferents in vegetable products do not interfere with the nitration of 2-sec-butylphenol. Recoveries of nitrate from vegetable products were satisfactory and the standard deviation for the whole procedure was 1.41% for the 42 determinations; the detection limit of the method is 1.3 p.p.m. for nitratenitrogen.
8 citations
Patent•
02 Mar 1982
TL;DR: A polysaccharide contains D-glucose as main constitutive sugar according to gas chromatography carried out through methylation in accordance with the Hakomori's method; and hydrolysis and to paper chromatography performed through acid hydrolyisation.
Abstract: A polysaccharide contains D-glucose as main constitutive sugar according to gas chromatography carried out through methylation in accordance with the Hakomori's method; and hydrolysis and to paper chromatography carried out through acid hydrolysis shows G 1 →:→ 3 G 1 →:→ 3 G 6 1 →=0.38-0.43:0.14-0.24:0.38-0.43 in gas chromatographic mass spectrometry (GC-MS method); has the signal of C 1 at 103 ppm as measured from a 13 C-nuclear magnetic resonance spectrum; shows characteristic absorption at 1720 cm -1 -1760 cm -1 and 890 cm -1 in an infrared absorption spectrum; has number average molecular weight between 50,000 and 500,000 as measured by the osmotic pressure; has a specific rotation [α] D 25 between +20 and +70; consists 42.0 to 45.0% of carbon, 5.7 to 6.7% of hydrogen, 0 to 1.0% of nitrogen, 0.2 to 0.8% of ash content and the rest of oxygen according to the results of an elementary analysis; contains 4.0 to 6.0% of phosphoric acid in total as measured by a fluorescent X-ray analysis, the Allen's improved method, and the Deniges-Atkins method after carbonic acid melting; shows a color reaction of saccharides through an α-naphthol-sulfuric acid reaction, an indole-sulfuric acid reaction and a phenol-sulfuric acid reaction; is negative in a ninhydrin reaction, a carbazole-sulfuric acid reaction and an Elson-Morgan's reaction; is soluble in dimethyl sulfoxide (DMSO); swells well, though not readily soluble in water; and is insoluble in ethanol, pyridine, chloroform and other organic solvents.
6 citations
TL;DR: The rate of development of Ruhemann's purple in the ninhydrin reaction of two deuterated primary amines is significantly reduced, which appears to be a primary isotope effect and indicates that the cleavage of the carbon-hydrogen bond at the α-position is involved in the rate-determining step of the color reaction.
Abstract: The rate of development of Ruhemann's purple in the ninhydrin reaction of two deuterated primary amines, αα-d2-p-tyramine and αα-d2-β-phenylethylamine, is significantly reduced It appears to be a primary isotope effect and indicates that the cleavage of the carbon-hydrogen bond at the α-position is involved in the rate-determining step of the color reaction.
6 citations
Patent•
24 Feb 1982
TL;DR: A physiologically active novel substance designated as Mutastein is disclosed in this paper, which has the following specific physiological properties: (1) it has a molecular weight of at least 200,000, and when subjected to gel-filtration with Sephadex G-100 and Sephallose 6B, it will be eluted in the void volume.
Abstract: A physiologically active novel substance designated as Mutastein is disclosed, which has the following specific physiological properties: (1) it has a molecular weight of at least 200,000, and when subjected to gel-filtration with Sephadex G-100 and Sephallose 6B, it will be eluted in the void volume; (2) its ultraviolet spectrum: FIG. 1; (3) its infrared spectrum: FIG. 2; (4) it has a proteinous nature and contains about 10% of saccharide; (5) it is soluble in water and a saline solution, but it will be salted out from a saturated solution containing about 30% of ammonium salfate; and it is insoluble in acetone, ethanol, ethylacetate and benzene, (6) it dissolves in a buffer solution having a pH 7 or over, but it precipitates at a pH of from 3 to 3.5, (7) it is stable when heat-treated at pH 9, at 100° C. for 10 minutes, (8) its elementary analysis: C: about 44%, H: about 7%, and N: about 12%, (9) its color reactions: Phenol-surfuric acid color reaction (orange), and Folin's color reaction (blue). This substance can be prepared by culturing the Mutastein-producing strain belonging to the genus Aspergillus, and isolating the Mutastein from the culture medium.
5 citations
Patent•
20 Apr 1982
TL;DR: Polysaccharide PS-A isolated from the plant of genus Epimedium violaceum Morr. et al. as mentioned in this paper have the following properties, process for its preparation, and an infection- preventing agent and an immunostimulating agent containing same as an effective ingredient.
Abstract: Polysaccharide PS-A isolated from the plant of genus Epimedium violaceum Morr. et decne. having the following properties, process for its preparation, and an infection- preventing agent and an immunostimulating agent containing same as an effective ingredient: 1. Elemental analysis: C = 40,92, H = 6.17, ash = trace; 2. Molecular Weight: 75,000 + 25,000 (mean molecular weight); 3. Decomposition point: 205°C; 4. pH: 7.0 (100 mg of PS-A in 50 ml of water); 5. Specific rotary power: [α] 19 = -23.6°C (in H 2 O,c = 0.527); 6. IR absorption spectrum: v KBr max (cm -1 ): 3400, 2900, 1620, 1400, 1230, 1060; 7. UV absorption spectrum: no maximum absorptions in 240 to 400 nm; 8. Appearance: white to slightly brown amorphous powder; 9. Solubility: soluble in water, insoluble in methanol, ethanol, acetone, ethyl, acetat, diethyl ether, hexane, and chloroform; 10. Color reaction: positive for the following reactions: (a) anthrone test, (b) Molisch reaction, (c) skatole reaction, (d) Bial reaction; negative for the following reactions: (a) ninhydrin reaction, (b) 2,4-DNP reaction, (c) Selivanoff reaction, (d) naphthoresorcin reaction, (e) carbazole reaction; 11. Constitutive sugar: arabinose and galactose; 12. Uniformity, uniform in ultracentrifugation, electrophoresis, and gel filtration.
5 citations
Patent•
15 May 1982
TL;DR: In this paper, a carboxylic acid halide of formulaI(R is alkyl, aryl or aralkyl; X is halogen) is reacted with hydroxylamine in water as a solvent at room temperature to give hydroxamic acid of formula II.
Abstract: PURPOSE:To obtain the titled substance useful as a color reaction testing reagent, precipitate forming reagent, medicine, agricultural chemical, etc. in high yield by an easy operation, by reacting a carboxylic acid halide with hydroxylamine in water as a solvent at room temperature. CONSTITUTION:A carboxylic acid halide of formulaI(R is alkyl, aryl or aralkyl; X is halogen) is reacted with hydroxylamine in water as a solvent at room temperature to give hydroxamic acid of formula II. In case the hydroxylamine is used in the form of a salt, e.g. hydrochloride or sulfate, an equivalent amount of a basic substance, e.g. NaOH or Na2CO3, is simultaneously used. EFFECT:No removing and separating operation is required due to the metallic halide as a by-product dissolved in water, and the metallic halide will not be contained in the aimed substance. No trouble, e.g. danger, of fire in the absence of an organic solvent used.
4 citations
2 citations
Patent•
22 Sep 1982
TL;DR: Lysopeptin A(salt) having the following properties: elemental analysis (%): C 55.53, H 5.97, N 14.58, O 23.25, D -61.9+ or -2.0 deg.
Abstract: NEW MATERIAL:Lysopeptin A(salt) having the following properties. Elemental analysis (%): C 55.53, H 5.97; N 14.58; O 23.25. Molecular weight: 1,100. Specific rotatory power:[alpha]D -61.9+ or -2.0 deg.C. Solubility: soluble in water, DMF, dimethyl sulfoxide, insoluble in ethanol, acetone, ethyl acetate, chloroform, and ether. Color reaction: positive in ninhydrin reaction Pauli's reaction, Ehrlich's reaction, ferric chloride-potassium ferricyanide reaction, and negative in Sakaguchi's reaction. An amphoteric substance. Colorless. Hydrolysis product: tryptophan, alpha-amino-beta-methylamino-butyric acid, beta-alanine metatyrosine. Lysopeptin B(salt) having elemental analysis and specific rotatory power different from those of lysopeptin A. USE:An antibacterial agent against Gram-negative bacteria. PROCESS:Streptomyces chartreusis PA-40705 (FREM-P NO. 5864) is cultivated in a medium and the titled antibiotic is collected from the culture.
2 citations
Patent•
20 Mar 1982
TL;DR: A strain of Streptomyces sb-AM-3603 (FERM-5619) is aerobically cultured in a medium and the culture filtrate is subjected to adsorption with activate carbob, porous synthetic high-polymer resin or ion-exhange resin this article.
Abstract: NEW MATERIAL:Antibiotic AM-3603; elementary analyses: C, H, N, O; melting point: 95-97 deg.C; specific rotatory power [alpha]D =+9 deg.(c=1, ethanol); solubility: soluble in methanol, pyridine, insoluble petroleum ether, n-hexane and water; color reaction: positive to anisaldehyde-sulfuric acid reaction and negative to Reyden- Smith reaction; a white powder. USE:Antimicrobial and remedy for a part of gram-positive baceteria, phytopathogenetic eumycetes and dermatozoonosis eumycetes. It also is used for agricultural purpose and as a regent. PREPARATION:A strain of Streptomyces sb-AM-3603 (FERM-5619) is aerobically cultured in a medium and the culture filtrate is subjected to adsorption with activate carbob, porous synthetic high-polymer resin or ion-exhange resin. Then, the elution is conducted, the eluate is extracted and concentrated to dryness to give a crude powder of AM-3603. Further, purification is effected by combination of silica-gel column chromatography and other processer to obtain the antibiotic AM-3603.
2 citations
Patent•
25 May 1982
TL;DR: In this article, a bacterium capable of producing high polymer polysaccharide substance MPS-82 substance is subjected to settled culture under anaerobic conditions, and after the cultivation is over, the desired substance is separated and purified by centrifugation, extraction, ion exchange chromatography, etc.
Abstract: NEW MATERIAL:High polymer polysaccharide substance MPS-82 having the following physical and chemical properties. Elemental analysis: C 38.03%, H: 6.52%, O: 55.21%, N: 0.24%. Molecular weight: 2.0S (S: Svedberg unit (ultracentrifugation). Melting point: 225°C (change in color)W270°C(decomposition). Specific rotatory power: [α]D 24 =+112±5° (c=0.25%). Solubility: soluble in water, insoluble in methanol, acetone, etc. Color reaction: positive in Molisch reaction and anthrone reaction. pH: 4.75. (0.5wt% aqueous solution). Appearance: white, fibrous state. USE: An immuno activator. An antitumor agent. PROCESS: A bacterium [e.g., Bifidobacterium longum No. 21-R (FERM-BP-126)] belonging to the genus Bifidobacterium, capable of producing high polymer polysaccharide substance MPS-82 substance is subjected to settled culture under anaerobic conditions, and after the cultivation is over, the desired substance is separated and purified by centrifugation, extraction, ion exchange chromatography, etc. COPYRIGHT: (C)1983,JPO&Japio
TL;DR: By using TBPE as ion-pairing counter-ion whose absorption spectrum differs markedly from that of the resulting ion-pairs, ion pair high pressure liquid chromatographic determination of many organic bases was carried out sensitively without the absorptive background of TBPE added in the mobile phase as mentioned in this paper.
Abstract: By using TBPE as ion-pairing counter-ion whose absorption spectrum differs markedly from that of the resulting ion-pairs, ion pair high pressure liquid chromatographic determination of many organic bases was carried out sensitively without the absorptive background of TBPE added in the mobile phase.
Patent•
16 Dec 1982
TL;DR: In this paper, a pencil for writing on a receiving sheet coated with an electron acceptor consisting of a dye precursor which gives a chemical colour reaction with the dye acceptor, and a solid or liquid carrier and customary additives, the dye precursor being treated with a metal salt which gave a metal complex compound, the weight ratio of metal salt to dye precursor was 1:10 to 1:100.
Abstract: Pencil for writing on a receiving sheet coated with an electron acceptor consisting of a dye precursor which gives a chemical colour reaction with the dye acceptor, and a solid or liquid carrier and customary additives, the dye precursor being treated with a metal salt which gives a metal complex compound, the weight ratio of metal salt to dye precursor being 1:10 to 1:100.
Patent•
27 Apr 1982
TL;DR: In this paper, an amylase-containing solution to be tested is added to the substrate, and a decomposition product of the substrate is formed, which can be determined or this product may be reacted with a reagent for color reaction of the reducing type hydrogen transfer system, e.g. tetrazolium salt and diaphorase.
Abstract: PURPOSE:Accurate measurement of the titled substance capable of being automatized completely, wherein a decomposition product of a substrate to be formed by a reaction is measured by using a glucose polymer or cyclic glucose polymer containing a modified reducing terminal glucose residue as the substrate. CONSTITUTION:A glucose polymer or cyclic glucose polymer having at least a polymerization degree of >=5, containing a modified reducing terminal glucose residue is used as a substrate, an amylase-containing solution to be tested is added to the substrate, and a decomposition product of the substrate is formed. When the decomposition product of the substrate is treated with maltose dehydrogenase and NAD or NADP, a reducing type NAD or a reducing type NADP is formed quantitatively. This formed product may be determined or this product may be reacted with a reagent for color reaction of the reducing type hydrogen transfer system, e.g., a reagent containing a tetrazolium salt and diaphorase, so that the product is colored, and determined to measure amylase activity.
Patent•
[...]
13 Feb 1982
TL;DR: In this paper, Sclerotia of tuckahoe in Basidiomycetes are cultured to produce mycelia and the mycellia are extracted with water or aqueous alkali and subjected to specific fractionation operation to give β-D-glucan with high antitumor activity.
Abstract: PURPOSE: Sclerotia of tuckahoe in Basidiomycetes are cultured to produce mycelia and the mycelia are extracted with water or aqueous alkali and subjected to specific fractionation operation to give β-D-glucan with high antitumor activity. CONSTITUTION: Sclerotia of tuckahoe in Basidiomycetes (FERM-4766) are cultured and the resultant mycelia are extracted with water or aqueous alkali and the extract is fractionated to give β-D-glucan. The resultant β-D-glucan has following properties: causing color reaction as a saccharide in the phenol-sulfuric acid reaction, anthrone-sulfuric acid reaction, α-naphthol-sulfuric reaction and orcinol-sulfuric acid reaction; molecular weight of 2,000,000W6,000,000 according to the light-scattering method; specific rotatory power, -10°W-50°; when the molar number of the nonreductive terminal G 1 - (G stands for glucose) is set to 1, the mole number of - 3 G 1 - is 2.5W7, that of - 3 G 1 6 - is 0.5W2.5, - 3 G 1 4 - is 0W0.4 and 6 G 1 - is 0.3W2.5. COPYRIGHT: (C)1983,JPO&Japio
Patent•
22 May 1982
TL;DR: The titled substance is an acidic substance as it is, and has the following characteristics as Na salt: appearance, colorles acicular crystal; melting point, 143-145 deg.C; elemental analysis, C 59.96%, H 9.03%, O 27.60%, Na 3.41%;[alpha]D =+5.5 deg.
Abstract: NEW MATERIAL:The titled substance is an acidic substance as it is, and has the following characteristics as Na salt: appearance, colorles acicular crystal; melting point, 143-145 deg.C; elemental analysis, C 59.96%, H 9.03%, O 27.60%, Na 3.41%;[alpha]D =+5.5 deg. (C=1, CHCl3); solubility, soluble in methanol, chloroform, acetone, benzene, etc., insoluble in water; color reaction, positive to 2,4-dinitrophenylhydrazine reaction, negative to ninhydrin reaction and vaniline-sulfuric acid reaction; colors with I2 vapor; etc. USE:Antibacterial agent especially effective to Gram-positive bacteria. PROCESS:Bacterial strain of Streptomyces genus, e.g. Streptomyces sp. 5075 strain (FERM-P No.62), etc. is cultured at 30 deg.C for 4-8 days pref. under aeration and agitation. The objective substance is separated from the product and purified.