Showing papers on "Color reaction published in 1984"
TL;DR: The colour reactions of three oximes with ammine- and nitrosyl-pentacyanoferrate(II) ions have been studied and the optimum reaction conditions, spectral characteristics and molar compositions have been found and some instability constants determined.
12 citations
TL;DR: In this article, a simple, sensitive and rapid spectrophotometric method has been developed for the determination of osmium using cyclohexane 1,3-dione bisthiosemicarbazone mono-hydrochloride (1.3-CHDT).
Abstract: A simple, sensitive and rapid spectrophotometric method has been developed for the determination of osmium using cyclohexane 1,3-dione bisthiosemicarbazone mono-hydrochloride (1,3-CHDT.HCl). The method is based on the instantaneous colour reaction between 1,3-CHDT. HCl and osmium(VIII) in sodium acetate—acetic acid buffer medium (pH range 3–6). The osmium complex shows maximum absorbance at 375 nm and considerable absorbance at 510 nm. Although the complex formed between platinum(IV) and reagent (1,3-CHDT.HCl) shows maximum absorbance at 375 nm, it does not show any absorbance at 510 nm. Simultaneous determination of osmium and platinum is carried out when present alone and in presence of other foreign (associated) ions. Some physico-chemical and analytical characteristics of osmium and platinum complex are described. Interference of various foreign ions have studied and osmium is estimated selectively in the presence of constituents of platinum ores.
11 citations
TL;DR: A sensitive colour reaction of copper with Cadion 2B in the presence of Triton X-100 and its application to the spectrophotometric determination of cyanide in waste water are described.
Abstract: A sensitive colour reaction of copper with Cadion 2B in the presence of Triton X-100 and its application to the spectrophotometric determination of cyanide in waste water are described.
11 citations
9 citations
TL;DR: In this article, a highly selective method has been developed for the extraction and spectrophotometric determination of microgram quantities of iron(III) in the presence of azide to give a violet-coloured complex insoluble in water.
Abstract: N1-Hydroxy-N1,N2-diphenylbenzamidine (HDPBA) reacts with iron(III) in the presence of azide to give a violet-coloured complex insoluble in water. The mixed ligand complex having a composition of 1:2:2 (Fe:HDPBA:N3−) is quantitatively extracted into chloroform at pH 2–5. The violet-coloured complex has an absorption maximum at 530 nm with a molar absorptivity of 4900 l mol−1 cm−1. On the basis of this sensitive colour reaction, a highly selective method has been developed for the extraction and spectrophotometric determination of microgram quantities of iron(III). The effect of foreign ions and several experimental variables have been studied. The method is simple, rapid, precise, and free from the regid control of experimental parameters.
6 citations
TL;DR: In this article, a microqualitative detection combining the colour reaction with p-dimethylaminobenzaldehyde (pDAB) with cation-exchange was proposed.
Abstract: Recently, ion-exchange resins have been used for the detection of uranium [11, nitroso compounds [21, EDTA [3], silver [4], barbiturates [5], mercapto compounds [6], hydroxamic acids [7], hydrazines [8], and zinc [9] by the "Resin Spot Test Technique". Many methods for the detection of indole and pyrrole are based on the colour reaction with p-dimethylaminobenzaldehyde (pDAB). However, these procedures are often of limited selectivity and are lacking simplicity. We have therefore worked out a microqualitative detection combining the colour reaction with p-DAB with cation-exchange. Accordingly, pyrrole derivatives react analogously to pyrrole, whereas 50 gg of isatine, acetphenolisatine and strychnine do not react. We found that under our experimental conditions the quinoidal violet compound is formed on the surface of weakly acidic macroreticular resin. It is essential that the resin grains are first treated with the analyte solution and subsequently with p-DAB solution as otherwise no satisfactory results are obtained. Various types of resins have been tested and Amberlite IRC-50 (in H-form) has been found to give best results (i.e. the most favourable limit of identification). The coloured product is stable within the pH range of 1.4 to 5.3 and can still be clearly recognized even after 60 min. Limits of identification and other characteristics are shown in Table 1. The intensity of the colour depends on the amount of analyte and the reaction time. The selectivity of the proposed procedure was examined by using 106 typical organic compounds. Tests have shown that the following compounds do not cause interferences: alcohols, aldehydes, aliphatic amines, amides, amino acids, carboxylic acids, esters, heterocyclic bases, ketones, nitro compounds, phenols, sulphonic acids and thio compounds. Certain compounds can only be tolerated up to a certain amount (see Table 2).
6 citations
TL;DR: In this article, a sensitive color reaction for the determination of ethyl acetoacetate in aqueous solution is described, which relies on the reaction of the determinand with diazotised anthranilic acid in the presence of hydrogen carbonate.
Abstract: A sensitive colour reaction for the determination of ethyl acetoacetate in aqueous solution is described. It relies on the reaction of the determinand with diazotised anthranilic acid in the presence of hydrogen carbonate. An intensely colured water-soluble compound is rapidly formed for which a maximum absorbance occurred at 315 nm. Beer's law was obeyed between 10 and 200 µg of ethyl acetoacetate per 25 ml final volume (i.e., 0.4–8 p.p.m.) and a molar absorptivity of 2.59 × 104 l mol–1 cm–1 was found. A relative error of +2.2 to –0.8% and a relative standard deviation of 2.4–0.2%, depending on the concentration level, were also observed. Interference due to foreign organic compounds has also been examined.
4 citations
TL;DR: The reaction of Ni (II) dithiocarbamate chelate formation was applied as a color reaction in high-performance liquid chromatography (HPLC) analysis of aliphatic primary and secondary amines and microdetermination of stimulant drugs, e.g. methamphetamine and amphetamine, in urine.
Abstract: The reaction of Ni (II) dithiocarbamate chelate formation was applied as a color reaction in high-performance liquid chromatography (HPLC) analysis of aliphatic primary and secondary amines. The reaction proceeded quantitatively and almost instantaneously at room temperature in alkaline media. The deep yellow (loge=4.5 at 325 nm) reaction products were extractable with organic solvents and gave sharp peaks on both normal-phase and reverse-phase chromatography. This method was applied to the microdetermination of stimulant drugs, e.g. methamphetamine and amphetamine, in urine, and as little as 1 ng of these drugs could be detected.
3 citations
TL;DR: In this paper, a color reaction of purine bases utilizing a ternary-complex system among a purine base, a metal ion, and a xanthene dye was studied.
Abstract: A color reaction of purine bases utilizing a ternary-complex system among a purine base, a metal ion, and a xanthene dye was studied, and the fundamental conditions for the spectrophotometric determination of purine bases(as adenine) with eosin and silver(I) were established. The absorption maxima of the ternary-complexes lie at 550–560 nm against water. The optimum pH ranges for the color formation of the complexes are pH 4.8–5.4. Beer’s law is obeyed over the range of 1–10 μg/10 cm3 of adenine, and the apparent molar absorptivity is 1.1×105 dm3 mol−1cm−1 at 560 nm. In the determination of adenine, the coexistence of pyrimidine bases, such as uracil and cytosine, and nucleotides containing adenine, such as 5′-adenosine triphosphate(ATP) and β-nicotinamide–adenine dinucleotide(NAD), scarcely interfere.
3 citations
Patent•
03 Feb 1984
TL;DR: In this article, Nocardia blackwellii MG-323 hF2(FERM-P 6606) is inoculated into a nutritive medium, cultivated at 4.5-8.0pH at 25-35 deg.C.
Abstract: NEW MATERIAL:Antibiotic indisocin and its N-methyl derivative having the following physical and chemical properties. Molecular formula: C13H9ClN2O3. Specific rotatory power [alpha] D=+20+ or -5 deg. (C 0.03, methanol). Infrared absorption spectrum measured in CCl4 solution shown by the figure. Solubility: soluble in methanol, acetone, ethyl acetate, chloroform, benzene, etc., slightly soluble in water. Color reaction: positive in Rydon Smith reaction, sulfuric acid, iodine, and cameleon reactions, negative in ninhydrin and TTC (2,3,5-triphenyltetrazolium) chloride reactions. USE:An antibacterial agent having high antibacterial activity against Gram-positive and Gram-negative bacteria. PROCESS:A bacterium [e.g., Nocardia blackwellii MG-323 hF2(FERM-P 6606)] belonging to the genus Nocardia, capable of producing antibiotic indisocin, is inoculated into a nutritive medium, cultivated at 4.5-8.0pH at 25-35 deg.C, indisocin is collected from the culture, and subjected to N-methylation, if necessary.
3 citations
TL;DR: In this paper, the coupling reaction of N-(1-naphthyl)ethylenediamine (N-NED) with diazotised 4-aminoacetophenone in acidic media has been studied in order to determine its ability to serve as the basis of a sensitive spectrophotometric method for the determination of N -NED in aqueous solution.
Abstract: The coupling reaction of N-(1-naphthyl)ethylenediamine (N-NED) with diazotised 4-aminoacetophenone in acidic media has been studied in order to determine its ability to serve as the basis of a sensitive spectrophotometric method for the determination of N-NED in aqueous solution. The intense reddish purple monoazo dye formed shows a maximum absorption at 550 nm, and is water soluble and stable. A graph of absorbance versus N-NED concentration is linear, indicating that Beer's law is adhered to over the range 4.4–88 µg of N-NED in a final volume of 25 ml, i.e., 0.18–3.5 p.p.m. of N-NED, with a molar absorptivity of 5.61 × 104 l mol–1 cm–1, a sensitivity index of 0.0033 µg cm–2, a relative error from -0.9 to +3.8% and a relative standard deviation from 0.2 to 4%, depending on the N-NED concentration. The optimum conditions for the colour reaction and the interference of foreign organic compounds have been investigated. The method is simple and rapid and needs neither temperature control nor solvent extraction techniques.
TL;DR: In this paper, the colour reaction of magnesium(II) with Khimduchlorophosphonazo-I (KCPA-I) has been studied spectrophotometrically with a view to developing a procedure for the determination of exchangeable magnesium in acidic soils.
Abstract: The colour reaction of magnesium(II) with Khimduchlorophosphonazo-I (KCPA-I) has been studied spectrophotometrically with a view to develop a procedure for the determination of exchangeable magnesium in acidic soils.
TL;DR: In this paper, a spectrophotometric method for sulfur dioxide determination was explored on the basis of its complexation with TNP-amino acid derivatives forming an orange colored 1:1 complex with an increase in absorbance at 420 nm.
Abstract: A spectrophotometric method for sulfur dioxide determination was explored on the basis of its complexation with TNP-amino acid derivatives forming an orange colored 1:1 complex with an increase in absorbance at 420 nm. TNP-glycine, TNP-threonine, TNP-serine and TNP-histidine were investigated. The color development was instantaneous and the absorbance remained unchanged even after 24 hrs of mixing when kept in the dark. Linear calibration graphs (0-5 × 10−5M sulfite ions) were obtained at optimal reaction conditions of 7 × 10−5M TNP-amino acid and pH 8.0 phosphate buffer (0.05 M). The investigation of the effect of several diverse ions revealed an interference by sulphide and mercury (II) ions at concentration levels of 10−4M. The standard deviation of determining 3 × 10−5M sulfite solution (10 times) was 1.474 × 10−7 M.
TL;DR: A color reaction between polyamines and ο-hydroxyhydroquinonephthalein-copper(II) complex, and conditions for the determination of spermine or spermidine were studied.
Abstract: A color reaction between polyamines and ο-hydroxyhydroquinonephthalein-copper(II) complex, and conditions for the determination of spermine or spermidine were studied. The apparent molar absorptivities at 585 nm were 7.0 × 10 5 1 mol-1 cm -1 for spermine and 3.0 × 105 1 mol-1 cm-1 for spermidine, respectively.
TL;DR: The conversion of nitrate into nitrosyl chloride, and subsequent reaction with 8-hydroxyquinoline and p-aminobenzene sulphonic acid allows the rapid detection of down to 5 ng of nitrates in a spot-test procedure as discussed by the authors.
Abstract: The conversion of nitrate into nitrosyl chloride, and subsequent reaction with 8-hydroxyquinoline andp-aminobenzene sulphonic acid allows the rapid detection of down to 5 ng of nitrate in a spot-test procedure. Over 55 anions and cations have been shown not to interfere if detection is preceded by thin-layer chromatography on silica. The method can be used for the determination of nitrate in food and water samples.
Patent•
29 Oct 1984
TL;DR: Svedberg et al. as discussed by the authors proposed an inhibitor against rise in blood pressure containing high polymer polysaccharide substance MPS-80 as an active ingredient, which is effective for remedying and preventing arteriosclerosis or hypertension An oral dose is 1-10g/kg/day
Abstract: PURPOSE:The titled inhibitor containing high polymer polysaccharide substance MPS-80 as an active ingredient CONSTITUTION:An inhibitor against rise in blood pressure containing high polymer polysaccharide substance MPS-80[elemental analysis: C=422wt%, H= 69wt%, and O=504wt%; molecular weight: 798S (S: Svedberg) (by centrifugation method); melting point (decomposition point): change in color starts at 262 degC, and changes into black at 263-264 degC; specific rotatory power: [alpha] D=+332 (C= 05wt%); solubility: soluble in water, insoluble in methanol, etc; color reaction: positive in Molish reaction, anthrone reaction, etc, and negative in aniline- hydrochloric acid reaction, carbazole-sulfuric acid reaction, etc; pH: 01-05wt% aqueous solution is neutral: lyophilized substance is white and fibrous] as an active ingredient This is effective for remedying and preventing arteriosclerosis or hypertension An oral dose is 01-10g/kg/day
TL;DR: The color reaction of Am(III) with Arsenazo III in several hydroorganic media has been examined systematically on the addition of certain polar water-miscible organic solvents in the course of a search for improved and simple spectrophotometric methods for the estimation of americium as discussed by the authors.
Abstract: The colour reaction of Am(III) with Arsenazo III in several hydroorganic media has been examined systematically on the addition of certain polar water-miscible organic solvents in the course of a search for improved and simple spectrophotometric methods for the estimation of americium. Addition of these substances resulted in the stabilization of colour and brought about a drastic enhancement in the absorbance values. The organic additives studied include acetone, acetonitrile, dimethylformamide, dioxane and ethanol. Among the many solvents tested, alcohol and dioxane proved to be the most effective; highest sensitivity is obtained by using a 60% dioxane-ethanol (1∶1) mixture. The apparent molar absorptivity based on Am content is 184616±9931 mol−1 cm−1 at 655 nm which is about 3 times that attained for the reaction in aqueous medium (65178±1243). Strikingly, this is the highest value reported as yet for its determination. Beer's law is obeyed both in mixed as well as aqueous media. The effects of some experimental variables on colour development have also been studied to optimize the conditions for the assay of Am.
TL;DR: The results of the aldehydet determination in photographic gelatin by colour reaction with thiobarbituric acid are influenced by the composition of the solvent used for the reaction and are not specific characteristics of the analyzed gelatin this article.
Abstract: The yield of the gelatin-thiobarbituric acid reaction depends on the water content of the solvent. Under these conditions the results of the aldehydet determination in photographic gelatin by colour reaction with thiobarbituric acid are influenced by the composition of the solvent used for the reaction and are not specific characteristics of the analyzed gelatin.
Patent•
29 Aug 1984
TL;DR: A color reaction adjuvant may be added to a brush pen, a sponge or the like for supplying water for color developing, discoloration or decolorization at the time of use as mentioned in this paper.
Abstract: PURPOSE:To obtain a printed matter such as a question book in which an answer is checked when required, a color-changing glass or the like, by printing in an ink containing a color reaction agent which is colorless or forms a color when being dried and forms a color, is discolored or decolorized when being supplied with water, and a color reaction adjuvant. CONSTITUTION:A pH indicator for indicating a pH range for a color reaction, a discolorable coloring matter for indicating the pH range, a flavonoid coloring matter showing a color change upon a redox reaction or presence or absence of a substituent group in addition to a change of pH, or the like is used as a color reaction agent, while an acidic, basic or neutral compound capable of adjusting pH and/or a reducing agent or the like is used as a color reaction adjuvant. The color reaction agent and the color reaction adjuvant are dissolved in a solvent such as water or an alcohol, to which a thickener or the like is added, and the resultant material is used as an ink for printing. The color reaction adjuvant may be mixed with the color reaction agent, or may be infiltrated into a material to be printed in the form of a separate solution. Further, the color reaction adjuvant may be added to a brush pen, a sponge or the like for supplying water for color developing, discoloration or decolorization at the time of use.
TL;DR: In this article, a new color reaction for detecting citric and tartaric acids in vinegar has been developed, which can detect traces of citric (2 μg) and tarsic acid (5 μg) in concentrated acetic and formic acids.
Patent•
13 Aug 1984
TL;DR: The antibiotic substance pinkramycin having the following physical properties: Appearance, pale yellow plate crystal; (B) melting point, 165 deg.C; (C) specific rotation, [alpha] D=4.41 (c=0.77, methanol); (D) elementary analysis (%), C 42.76, H 6.59, N 0.83; (E) molecular formula, C60H111-118NO51; (F) molecular weight, about 1,200 (gel filtration); (G) color reaction, positive to
Abstract: NEW MATERIAL:The antibiotic substance pinkramycin having the following physical properties. (A) Appearance, pale yellow plate crystal; (B) melting point, 165 deg.C; (C) specific rotation, [alpha] D=4.41 (c=0.77, methanol); (D) elementary analysis (%), C 42.76, H 6.59, N 0.83; (E) molecular formula, C60H111-118NO51; (F) molecular weight, about 1,200 (gel filtration); (G) color reaction, positive to aniline phthalate reaction, TTC-Na reaction, naphthoresorcinol reaction, and negative to ninhydrin reaction, Sakaguchi's reaction, etc.; (H) solubility, soluble in water, methanol, ethanol, ether, acetone, ethyl acetate, etc., and insoluble in petroleum ether, chloroform, etc. USE:An antibiotic substance. PREPARATION:A bacterial strain such as Streptomyces carzinostaticus var. F41 cloya (FERM-P No.2257, ATCC No.15945), etc., is cultured in a liquid medium under aeration and agitation, preferably at 25-30 deg.C and 7.5-5pH for 24-180hr.
Patent•
11 Dec 1984
TL;DR: New antibiotic 5057B and process for its preparation was described in this article, where the antibiotic was prepared by culturing Streptomyces bacteria, and collecting the antibiotic from the culture product.
Abstract: PCT No. PCT/JP81/00320 Sec. 371 Date Jul. 13, 1982 Sec. 102(e) Date Jul. 13, 1982 PCT Filed Nov. 6, 1981 PCT Pub. No. WO82/01707 PCT Pub. Date May 27, 1982.New antibiotic 5057B and process for its preparation. Antibiotic 5057B itself is an acidic material, and its sodium salt has the following physical and chemical properties: (1) Colorless needles (2) Melting point: 143 DEG -145 DEG C. (3) Elementary analysis (Found %): C, 59.96; H, 9.03; O, 27.60; Na, 3.41 (4) Specific rotation: [ alpha ]D25=+5.5 DEG (C 1, CHCl3) (5) Maximum absorption in ultraviolet absorption spectrum: lambda maxMeOH(E1 cm1%) 290 nm (0.88) (6) Characteristic absorption (cm-1) in infrared absorption spectrum (taken with the potassium bromide tablet: 3480, 3000, 2970, 2812, 1719, 1645, 1595, 1465, 1385, 1160, 1100, 1035, 980 (7) Solubility: soluble in methanol, ethanol, ethyl acetate, chloroform, ether, acetone, and benzene and so forth; insoluble in water (8) Color reaction: positive in the 2,4-dinitrophenylhydrazine reaction; negative in the ninhydrin reaction and the vanilline-sulfuric acid reaction; colors with I2 gas. The antibiotic is prepared by culturing Streptomyces bacteria, and collecting the antibiotic from the culture product.
Patent•
19 Nov 1984
Patent•
19 Nov 1984
Patent•
15 Oct 1984
TL;DR: In this paper, a test sample solution containing ADP is added to the reagent, which is heated at about 37°C for about 10min, absorbance is measured, and formed H 2 O 2 is measured to determine ADT.
Abstract: PURPOSE: To determine ADP by a simple operation in high accuracy, by treating a test specimen solution containing ADP with kinase and oxidase, and measuring formed H 2 O 2 . CONSTITUTION: As shown by the equations I and II, proper kinase and oxidase, an agent required from the reaction by the enzymes, and an agent necessary for color reaction of H 2 O 2 are dissolved in a buffer solution, and it is adjusted to about 7.0W7.5pH to give a reagent. Then, a test specimen solution containing ADP is added to the reagent, which is heated at about 37°C for about 10min, absorbance is measured, and formed H 2 O 2 is measured, to determine ADT. Any of well-known method can be applied as a method for measuring H 2 O 2 , for example, H 2 O 2 is reacted with peroxidase in the presence of a coloring substance, and the formed dyestuff is measured optically. By this method, each clinical examination can be carried out simply and accurately. COPYRIGHT: (C)1986,JPO&Japio
Patent•
05 Sep 1984
TL;DR: A bacterium such as Chromobacterium sp. Y-02910 I (FERM P 6899) is cultivated preferably at 15-30 deg.C at 6-8pH for 3-5 days by shaking culture as mentioned in this paper.
Abstract: NEW MATERIAL:Antibiotic Y-02910 I-beta having the following physical and chemical properties. The melting point: 81-82 deg.C. Specific rotatory power: [alpha]D = -54 (c=0.1, methanol). Solubility: easily soluble in methanol, chloroform, acetone, and ethyl acetate, slightly soluble in water, insoluble in hexane. Color reaction: positive in KMnO4, sodium nitroprusside, etc., and negative in Molish reaction, ninhydrin reaction, etc. USE:An antibacterial agent against Gram-positive and Gram-negative bacteria. PREPARATION:A bacterium such as Chromobacterium sp. Y-02910 I (FERM P 6899), etc. belonging to the genus Chromobacterium is cultivated preferably at 15-30 deg.C at 6-8pH for 3-5 days by shaking culture.