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Color reaction

About: Color reaction is a research topic. Over the lifetime, 1194 publications have been published within this topic receiving 19579 citations.


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Patent
10 Dec 2014
TL;DR: In this paper, a method and a reaction device for visual detection of isothermal amplification of nucleic acid is presented, which comprises the following steps: in a special hermetic reaction device, adding a reagent capable of decomposing pyrophosphoric acid into phosphoric acid, after reaction, under the condition of not uncapping, mixing the amplified reaction liquid with other selected color reagents which are additionally stored in the device in advance to react and observing the color of the mixture after further reaction.
Abstract: The invention discloses a method and a reaction device for visual detection of isothermal amplification of nucleic acid. The method comprises the following steps: in a special hermetic reaction device, adding a reagent capable of decomposing pyrophosphoric acid into phosphoric acid into a nucleic acid amplified reaction liquid in advance; after reaction, under the condition of not uncapping, mixing the nucleic acid amplified reaction liquid with other selected color reagents which are additionally stored in the device in advance to react and observing the color of the mixture after further reaction; and judging whether nucleic acid amplified reaction is carried out on a sample or not, wherein the selected color reagents are reagent combination capable of being in color reaction with phosphoric acid (radicals). The method disclosed by the invention is simple to operate and is quick and sensitive. The test result can be detected by virtue of naked eyes. The method is low in cost and makes up the defects in the existing technology for detecting nucleic acid amplification.

4 citations

Journal ArticleDOI
TL;DR: In this paper, two simple, rapid and sensitive spectrophotometric methods have been developed for determination of Haloperidol (HP) in pure form and pharmaceutical formulations, based on the fact that HP reacts with a Calcon (Cal) or Amido Black (AB), to form an ion-association complex in acidic buffer solution, giving pink color with Calcon and blue color with Amido black, the absorbance of chloroform extracted complexes were measured at 531 nm and 626 nm respectively.
Abstract: Two simple, rapid and sensitive spectrophotometric methods have been developed for determination of Haloperidol (HP) in pure form and pharmaceutical formulations. A good sensitive colour reaction has been developed, based on the fact that Haloperidol (HP) reacts with a Calcon (Cal) or Amido Black (AB), to form an ion-association complex in acidic buffer solution, giving pink color with Calcon and blue color with Amido Black, the absorbance of chloroform extracted complexes were measured at 531 nm and 626 nm respectively. The effects of analytical parameters on the reported systems were investigated. The complexation reactions were extremely rapid at room temperature and the absorption values remain unchanged up to 72 h. Beer's law was obeyed in the concentration ranges of 1.20 – 33.83 μg.ml-1 and 0.752 -30.00 μg. ml-1, detection limits were 0.142 and 0.085 μg.ml-1 and the molar absorptivity coefficients were 2 1817 and 2 3230 l.mol-1.cm-1 for Cal and A.B respectively. Recoveries were between 99.35–101.67% for HP-Cal and between 98.67–100.87% for HP-AB complexes. The two methods were successfully applied for the determination of commercially available haloperidol in pure and in pharmaceutical formulations (tablets and dropes) without interference from its excipients. Statistical comparison of the results of the proposed method with those of the reference method shows excellent agreement and indicates no significant difference in accuracy and precision.

4 citations

Patent
24 Feb 1996
TL;DR: In this paper, a method for the determination of iodide in aqueous solutions, preferably in urine, is described in that the sample solution is admixed with a chromogen and a peracid solution and the color reaction is evaluated visually or photometrically.
Abstract: A method for the determination of iodide in aqueous solutions, preferably in urine. The process is characterized in that the sample solution is admixed with a chromogen and a peracid solution and the color reaction is evaluated visually or photometrically. In the determination of iodide in urine, the sample is pretreated by means of a purified activated carbon.

4 citations

Journal ArticleDOI
TL;DR: The PNPG method was more rapid and more sensitive than other colorimetric methods and required a single chromogenic reagent and agreed well with the results obtained by other available methods for CPK determination.

4 citations

Patent
12 Apr 1994
TL;DR: In this paper, a semi-opaque needle-like crystal was obtained by culturing a fungus belonging to the genus Talaromyces, capable of producing the compound of the formula, such as Talaris retardatus F24811 (FERM P-14-103).
Abstract: PURPOSE: To obtain new substance BE-24811 useful as an antitumor agent, etc., having an inhibitory effect on proliferation. CONSTITUTION: A compound of the formula has the following properties. Properties: semi-opaque needle-like crystal. Molecular formula: C 18 H 21 O 4 Cl. Mass spectrum (high resolution E1-MS, m/z): 336. 1133 [M + ]. Ultraviolet light absorption spectrum: λ max [MeOH, nm(e)] 230 (22900), 293 (10900), 3,44 (5300). Infrared light absorption spectrum: ν max (KBr, cm -1 : 3167, 2927, 1672, 1628, 1608, 1421, 1248, 1113. Solubility: readily soluble in dimethyl sulfoxide, soluble in chloroform, ethyl acetate and methanol, insoluble in water. Distinction of acid, neutral or basic substance: acidic substance. Color reaction: positive in iodine reaction, potassium permanganate reaction and 2,4dinitrophenylhydrazine reaction. The compound of the formula is obtained by culturing a fungus belonging to the genus Talaromyces, capable of producing the compound of the formula, such as Talaromyces retardatus F24811 (FERM P-14,103). COPYRIGHT: (C)1995,JPO

4 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20227
20214
20206
20198
20186
20175