Color reaction

About: Color reaction is a research topic. Over the lifetime, 1194 publications have been published within this topic receiving 19579 citations.

A colour reaction distinguishing between adrenaline and nor-adrenaline on paper chromatograms.

Abstract: IN recent studies we were concerned with the detection of traces of nor-adrenaline in preparations of adrenaline derived from natural sources. Colorimetric methods1,2 proved to be of limited value with low concentrations of nor-adrenaline; but excellent results were obtained with paper chromatography, using modifications of the methods described by James and others3,4. The adrenaline samples are dissolved in methanol containing a minimum quantity of hydrochloric acid, and then transferred to paper strips for descending chromatography, or to circular disks of filter paper for chromatography by Rutter's technique5. Water-saturated phenol is used as the developing solvent in an atmosphere of hydrochloric acid4. The paper chromatograms are then dried and sprayed with an appropriate colour reagent.

Identification of 1,5-anhydroglucitol in thin-layer chromatograms.

Abstract: The chromatographical characteristics of 1,5-anhydroglucitol in thin-layer chromatograms can be studied using the customary carbohydrate solvents. Both hydrogen peroxide and sodium metaperiodate oxidation reactions were tested in order to find a specific colour reaction for the detection of the compound. 1,5-anhydroglucitol was readily converted by periodate into an intermediate product which produced an intense orange-red colour with diphenylamine aniline reagent. According to data obtained from periodate oxidation, IR spectroscopy and mass fragmentography, the intermediate product was a dialdehyde compound with a C6 structure, possibly formed through cleavage at C2-C3. The formation of a compound of this kind without chain cleavage in the periodate oxidation of C6 carbohydrates is uncommon. Periodate oxidation followed by diphenylamine-aniline reaction affords a sensitive and specific method for the detection of 1,5-anhydroglucitol.

Reagent for specific determination of fructosamine

Abstract: The present invention provides a process for the specific determination of the serum fructosamine content in blood or samples derived from blood by reaction with an appropriate color reagent and measurement of the color change thereby brought about, wherein, before the color reaction, non-specific reducing-acting and/or turbidity-causing sample components are removed at approximately neutral pH value, subsequently the pH is adjusted to a value of from 10 to 12 and the color reagent is added thereto. The present invention also provides a reagent mixture for the specific determination of the serum fructosamine content in blood or samples derived from blood, wherein it comprises a reagent for the removal of non-specific reducing-acting and/or turbidity-causing sample components, a rebuffering reagent with a buffer which has a pH value in the range of from 10.5 to 12.5 and a color reagent for the detection of fructosamine.