Color reaction

About: Color reaction is a research topic. Over the lifetime, 1194 publications have been published within this topic receiving 19579 citations.

Phosphoric ester of novel antibiotic oa-6129

Abstract: NEW MATERIAL:A compound expressed by formula I (R represents H, OH or hydroxysulfonyloxy) exhibiting the following various characteristics Color reaction; positive to Ehrlich's reagent, chloroplatinic acid reaction and molybdic acid reaction and negative to ninhidrin reaction Paper chromatography; Rf value 003(developing solvent; acetonitrile, HCl and EDTA) High-voltage filter paper electrophoresis: 11-12cm to the anode USE:An antibacterial agent having strong antibacterial potency and beta-lactamase inhibitory activity PREPARATION:A material prepared by washing cultured bacteria of Brevibacteriumammoniagenes (ATCC 6871) is reacted with a substrate of an antibiotic OA-6129 A, B or C expressed by formula II under normal enzymic reaction conditions in the presence of adenosine-5'-triphosphate

Spot Test Detection of 1,2‐ and O‐Dioxo Compounds

Abstract: 1,2- and o-dioxo compounds condense with phenylhydrazine in mineral acid solution to give yellow phenyl osazones. The non quinoidic 1,2-dioxo compounds condense with ammonia and formaldehyde to give imidazoles which, as tertiary amines, show the Ohkuma color reaction with citric acid and acetic anhydride. Spot tests for pertinent dioxo compounds were developed on the basis of these facts.

Fundamental studies on clinical chemistry. vii. janovsky reaction of polynitrodiphenyl compounds.

Abstract: This work was undertaken to obtain a more satisfactory reagent for the assay of active methylene compounds than has been used. Biphenyl, diphenylmethane, diphenyl ether, diphenyl sulfide, diphenylsulfone and stilbene with nitro groups in 4, 4'-, 2, 4-, 2, 2', 4, 4'-positions were prepared and their color reactions with acetone and cyclohexanone in the presence of alkali were examined. 4, 4'-Dinitro compounds gave negative Janovsky reaction. Bis(p-nitrophenyl)-(IV) and bis(2, 4-dinitrophenyl)methane (V) gave blue color in methylcellosolve with addition of alkali without active methylene compound. 2, 4-Dinitro compounds showed bathochromic shift as compared with usual reagents such as m-dinitrobenzene, 1, 3, 5-trinitrobenzene and picric acid. The intensities of colors by these reagents were lower. 2, 2', 4, 4'-Tetranitro compounds showed higher intensities and sufficient stabilities of the color produced, but no bathochromic shifts. These tetranitro compounds also gave the specific color reactions for active methylene compounds such as acetone, cyclohexanone, 17-ketosteroids, creatinine and cardiac glycosides. 2, 2', 4, 4'-Tetranitrobiphenyl (III) could be the reagent that has higher sensitivity, sufficient stability and specificity in color reaction of active methylene compounds with alkali.

Novel macrolide antibiotic substance mycinamicins and production thereof

Abstract: NEW MATERIAL:The compound of formula (R is H or OH) or its salt. Mycinamicin X has the following physical and chemical properties. Color and appearance, white powder; molecular formula, C42H70N2O15S; elemental analysis (%), C 56.51, H 8.23, N 3.11, S 3.44; molecular weight, 875.08; TLC (silica gel), 0.52; color reaction, positive to decoloration of KMnO4 aqueous solution and negative to ninhydrin reaction, Sakaguchi's reaction and FeCl3 reaction; solubility, soluble in water and alcohol such as methanol, hardly soluble in acetone and insoluble in ethyl acetate, benzene, etc.; acidity or basicity, ampholytic; etc. USE:Antibacterial agent. PREPARATION:The objective compound can be produced by culturing a microbial strain belonging to Micromonospora genus and capable of producing mycinamicins [e.g. Micromonospora griseorubida A11725 (FERM BP-705)].