Topic
Color reaction
About: Color reaction is a research topic. Over the lifetime, 1194 publications have been published within this topic receiving 19579 citations.
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02 Dec 1981
TL;DR: In this article, a physiologically active substance CH-1 having interferon inducing capability and carcinostatic activity was extracted from Chlorella pyrenoidosa and the resulting precipitate is dissolved in water, and dialyzed.
Abstract: NEW MATERIAL:The physiologically active substance CH-1 having the following properties. Constitution, composed of at least C, H, O and N; elemental analysis, C 38.49%, H 6.07% and N 1.39%; molecular weight, nonpermeative to cellophane membrane and S=6.15S; melting point, develops color at 240°C; specific rotation,[α]D 29 -9.52 (c=1, water); solubility, soluble in water and insoluble in organic solvents such as methanol; color reaction, positive to anthrone reaction, etc. and negative to Folin-Lawry reaction, etc.; appearance, white powder; protein content, none; sugar content 53% in terms of glucose. USE: A physiologically active substance having interferon inducing capability and carcinostatic activity. PROCESS: Chlorella belonging to Chlorella genus, e.g. Chlorella pyrenoidosa, etc. is extracted with hot water. The extract liquid is added with a hydrophilic organic solvent to effect the solvent precipitation fractionation. The resultant precipitate is dissolved in water, and dialyzed. The dialyzed solution is subjected to the adsorption chromatography and gel filtration to obtain the objective physiologically active substance CH-1. COPYRIGHT: (C)1983,JPO&Japio
1 citations
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21 Nov 1989
TL;DR: A production fungus of polymeric polysaccharide F-B belonging to Fomes fomentarius (FERM-P 9704) is cultured and the aimed composition is collected from the cultured material.
Abstract: NEW MATERIAL:Polymeric polysaccharide F-B. Element analysis (%): F-B standard sample salt C 34.8, H 5.5, N 0.6, ash 11.6, F-B standard sample C 37.1, H 5.9, N 0.5, ash 5.2; range of molecular weight (gel filtration method); 7,000-17,000; average molecular weight (gel filtration method): 12,000; solubility: soluble in water, etc., insoluble in methanol, etc.; color reaction: positive to phenol sulfuric acid reaction, etc., negative to Ninhydrin reaction, etc.; color: white; constituted sugar and said composition: glucose:glucuronic acid=3.4:1; distinction of basic, acidic or neutral: 0.1% aqueous solution is acidic. USE:Anti-plant virus agent. Producible in large quantities, in a low cost and safety in fermentation industrially. PREPARATION:A production fungus of polymeric polysaccharide F-B belonging to Fomes fomentarius [preferably Fomes fomentarius JTS 3046 (FERM-P 9704)] is cultured and the aimed composition is collected from the cultured material.
1 citations
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29 May 1980
TL;DR: In this paper, the basic oligosaccharide amylostatins J, K, L, M and N have been obtained from the culture solution of a bacterium (e.g., "Streptomyces diastaticus var. amylstaticus" FERM-P 2499).
Abstract: NEW MATERIAL:Wealky basic oligosaccharide amylostatins J, K, L, M and N. Having values of elementary analysis different from each other amoung physical and chemical properties of amylostatins. Molecular weights as shown by the table. What are common to all; melting point: gradually are carbonized but not show clearly; having no specific absorption maximum at ultraviolet part; solubility: easily soluble in water, pyridine, etc., slightly soluble in ethanol, methanol, and insoluble in acetone, ether, etc.; color: white powder; color reaction: anthrone sulfuric acid reaction positive, ninhydrin reaction negative. USE:Having inhibiting actions on glycoside hydrolysis enzyme and immunity enhancing actions. PROCESS:A bacterium (e.g., ''Streptomyces diastaticus var. amylostaticus'' FERM-P 2499) belonging to the genus ''Streptomyces'' and having amylostatin producing ability is cultivated in a nutrient medium. The titled amylostains J-N are obtained from the culture solution.
1 citations
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TL;DR: Salicylamide (I) reacts with dimethylp-phenylenediamine, and K3Fe (CN)6 in an alkaline medium (phosphate buffer : pH 8.0) to give green coloration (indophenol dye) as mentioned in this paper.
Abstract: Salicylamide (I) reacts with dimethyl-p-phenylenediamine, and K3Fe (CN)6 in an alkaline medium (phosphate buffer : pH 8.0) to give green coloration (indophenol dye). This chromogen produced is extractable with chloroform, the extract showing an absorption maximum at 650mμ.A new spectrophotometric method, based on this color reaction, has been established to the determination of (I) in pharmaceutical preparations.Influences of 47 compounds were examined. The interferences from acetaminophene, ethoxybenzamide, salicylate, phenylephrine hydrochloride, sulpyrine, ascorbic acid, etc. are eliminated by the extraction with chloroform in an acidic medium. The recommended procedure is as follows. Sample {containing 100mg of (I)} is taken in a separatory funnel. To it are added 10ml of water and 10ml of 0.1 N HCl, and it is extracted 4 times with 30ml portions of chloroform. The extracts are combined and evaporated on a water bath. The residue is dissolved in 50ml of ethanol, and is made up exactly to 100ml with water. A 10ml aliquot is taken into another 100ml volumetric flask, and is made up to 100ml with ethanol. Ten ml of this solution is taken into another 100ml volumetric flask and prepared in the similar way.A 2 ml aliquot is transfered into a 30ml glass-stop-pered test tube. Five milliliters of buffer solution (phosphate buffer : pH 8.0), 2ml of 0.05% dimethyl-p-phenylenediamine solution and 1 ml of 1% K3Fe(CN)6 solution are added. After 5 min., the solution is shaken with 10ml of chloroform. The separated chloroform layer is dried with 2 g of Na2SO4, and is filtered. The absorbance is measured at 650mμ (ET) against chloroform. At the same time the absorbance of standard solution (ES) is determined, and the amount of (I) is calculated by ET/ES.
1 citations
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28 Apr 1987
TL;DR: In this paper, a bacterium (Streptomyces tanashiensis IM8442T strain (FERM P-8327), capable of producing antibiotic lactoquinomycin B, is subjected to air-ated culture preferably at 10-40 deg.C at 4-8pH for 24-144 hours and the antibiotic is collected from the culture mixture.
Abstract: NEW MATERIAL:Antibiotic lactoquinomycin B shown by the formula, its salt or a metal complex. Appearance: light yellow crystal. Molecular weight: 473. Molecular formula: C24H27NO9. Melting point: 149-152 deg.C (decomposition). Elemental analysis (%): C 58.91 H 5.81 N 2.77 O 29.63. Solubility: soluble in ether, chloroform, ethyl acetate, acetone, water, etc., insoluble in n-hexane. Color reaction: positive in magnesium acetate reaction, etc., negative in ninhydrin reaction, etc., Rf value: 0.30 (methanol). Specific rotatory power: [alpha] D+145.5 deg. (C 0.15, methanol), etc. USE:An antitumor agent. PREPARATION:A bacterium [e.g., Streptomyces tanashiensis IM8442T strain (FERM P-8327), etc.,] belonging to the genus Streptomyces, capable of producing antibiotic lactoquinomycin B, is subjected to airated culture preferably at 10-40 deg.C at 4-8pH for 24-144 hours and the antibiotic is collected from the culture mixture.
1 citations