Topic
Color reaction
About: Color reaction is a research topic. Over the lifetime, 1194 publications have been published within this topic receiving 19579 citations.
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TL;DR: The proposed method correlates with the method of Abell et al., has a repeatability of 0.7%, a day-to-day variability of 2.8% and a reproducibility between laboratories of 1%.
148 citations
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TL;DR: In this paper, the standard method for the colour development proposed is based on an investigation of the following variables: hydrogen sulphide losses, acidity at reaction and measurement, reagent concentrations, temperature at reaction, concentration of traces of heavy metals, time, and air-oxidation.
135 citations
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TL;DR: In this article, an ultramicro cholesterol technique based upon a new color reaction and requiring only 10 μl of serum is proposed, where the need for extraction with lipid solvent is eliminated by drying serum on filter paper segments and eluting directly in the ferrous sulfate color reagent.
132 citations
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TL;DR: In this article, a color reaction between pyrogallol red (PR) -molybdenum(VI) complex and protein was investigated, and a spectrophotometric method using the PR-moly bdenum complex could be used in the concentration range of 0 - 400 μg/10 ml of albumin, where the Sandell sensitivity was calculated to be 0.0048 μg/cm2 for albumin at 600 nm.
Abstract: A color reaction between pyrogallol red(PR) -molybdenum(VI) complex and protein was investigated. A spectrophotometric method using the PR-molybdenum(VI) complex could be used in the concentration range of 0 - 400 μg/10 ml of albumin, where the Sandell sensitivity was calculated to be 0.0048 μg/cm2 for albumin at 600 nm. A detection test of protein on a spot plate was also studied. The proposed method was applied to the determination of urinary protein; analytical results were satisfactory.
131 citations
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TL;DR: In this paper, the conditions necessary for the quantification of the ninhydrin color reaction have been defined and applied to the paper chromatographic determination of amino acids, and a reproducible, quantitative determination of micro amounts of amino acid was achieved.
120 citations