Topic
Color reaction
About: Color reaction is a research topic. Over the lifetime, 1194 publications have been published within this topic receiving 19579 citations.
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TL;DR: In this article, a blue color was obtained for fluoxetine with 0.01 mg/mL as visual limit of quantitation and provided a basis for a new spectrophotometric determination.
Abstract: Fluoxetine hydrochloride reacts with benzoyl peroxide and potassium iodide, after heating for 1 min at 30 °C, to give a blue colour having maximum absorbance at 570 nm. The reaction is selective for fluoxetine with 0.01 mg/mL as visual limit of quantitation and provides a basis for a new spectrophotometric determination. The colour reaction obeys Beer’s law from 0.1 mg/10 mL to 2.0 mg/10 mL of fluoxetine and the relative standard deviation is 0.68%. The qualitative assessment of tolerable amounts of other drugs is also studied.
10 citations
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TL;DR: It is concluded that triazenes with tissue aliamino groups play no significant part in the final color effect of azo coupling with the diazonium salts used.
Abstract: The suggestion that triazenes formed by union of alkalized diazonium salts with aliphatic amino groups contribute to the azo color reaction of tissue proteins is rejected. Deaminations of formaldehyde-fixed and other tissues were sufficient to render the previously oxyphil elements of these tissues basophilic to azure A-eosin B at the same pH level. After these deaminations there was no detectable difference in intensity or distribution of the azo coupling reaction. Freshly diazotized safranin 0, dimethylphenosafranin (methylene violet) and the diazosulfanilic acid, azure A sequence technic were used as testing methods. Acid extraction even when prolonged to 24 hr and when acid concentration raised from 0.1 N to 0.24 N does not alter the intensity or distribution of the several azo coupling reactions as compared with unextracted preparations. Deliberate creation of triazenes by admixture of proline, diethylamine, hydroxylamine, hydrazine sulfate, glycine and uric acid, added to the diazo in stoichiometric excess, more or less completely inhibited azo coupling of all tissue elements. Uric acid and hydrazine were the most eflective, and then hydroxylamine, proline and diethylamine in that order. Reacidification of the uric acid compound to pH 2.2-2.5 for 10 mm, filtration to remove liberated uric acid and realkalinization moderately restored the azo coupling capacity of diazosafranin. This illustrates acid destruction of a triazene of this diazotate. It is concluded that triazenes with tissue aliamino groups play no significant part in the final color effect of azo coupling with the diazonium
10 citations
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TL;DR: In this article, a simple flow-injection methodology is described for the determination of antimony using spectrophotometric detection, which is based on the enhancing effect of Antimony in the molybdenum blue reaction.
10 citations
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TL;DR: In this article, a new spectrophotometric method for the determination of palladium was described using 2-mercaptobenzoxazole as reagent, which has a sensitivity of 0.08 μg of Pd per cm 2 for log I 0 /I = 0.001 and obeys Beer's law from 2 to 40 p.p.m.
10 citations
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TL;DR: In this paper, a dry reagent strip technique for semiquantitative estimation of urea in adulterated milk is presented. But the technique is not suitable for the measurement of ammonia and carbon dioxide.
Abstract: +his-articl describes development and optimization of a visually evalua Ie dry reagent strip technique for semiquantitative estimation of urea in adulterated milk. It is based on (i) urease reacting with urea to liberate ammonia and carbon dioxide and (ii ) liberated ammonia reacting with a specific chromogen to change color of the strip from light yellow to magenta, which is visible with naked eyes. The technique is versatile as (i) it is used single step-working reagent to complete the reaction within 30 s at room temperature, (i i) gives different shades of color from yellow to magenta, depending upon concentration of urea present in the milk, (iii) this strip can measure urea concentration as low as 0.1 giL and (iv) thi s dry reagent strip is stable up to one year at room tempera~ur~
10 citations