Topic
Complementary DNA
About: Complementary DNA is a research topic. Over the lifetime, 55301 publications have been published within this topic receiving 2752650 citations. The topic is also known as: cDNA & DNA, Complementary.
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TL;DR: A λgt11 clone encoding prostate specific antigen has been isolated from a human prostate cDNA library and the primary structure shows extensive homology with proteases of the kallikrein family.
449 citations
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TL;DR: The structure of protein Kinase C is examined in an attempt to understand the molecular events connecting protein kinase C activation with the cellular response 1–3 and the deduced amino acid sequence for α, β and γ are closely related.
Abstract: We examined the structure of protein kinase C in an attempt to understand the molecular events connecting protein kinase C activation with the cellular response 1–3. Rabbit complementary DNA clones coding for three distinct types of protein kinase C, named α, β and γ, have been identified and sequenced. The deduced amino acid sequence for α, β and γ (673, 671 and 672 amino acids, respectively) are closely related. Kinases α and β share an identical TV-terminal sequence of 621 amino acid residues and their messenger RNAs arise from a single gene. The C-terminal halves of α, β and γ are protein kinase domains and are highly homologous to other protein kinases. The mRNAs for α, β and γ are expressed in various tissues with strikingly different tissue specificities. The one for γ is found ubiquitously among various tissues, while those for α and β predominate in the brain.
449 citations
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TL;DR: The predicted amino acid sequence indicates that formation of mature interleukin-3 involves proteolytic removal of not only the signal peptide but additional ammo-terminal amino acids.
Abstract: The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The predicted amino acid sequence indicates that formation of mature interleukin-3 involves proteolytic removal of not only the signal peptide but additional ammo-terminal amino acids.
449 citations
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TL;DR: Immunofluorescent microscopic localization indicated that after heat treatment, the levels of the 25-kD IAP were markedly increased and the protein was apparently associated with cytoplasmic granules, confirming that its expression is regulated by heat shock.
Abstract: The 25-kD inhibitor of actin polymerization (25-kD IAP), isolated from turkey smooth muscle (Miron, T., M. Wilchek, and B. Geiger, 1988. Eur. J. Biochem. 178:543-553), is shown here to be a low molecular mass heat shock protein (HSP). Direct sequence analysis of the purified protein, as well as cloning and sequencing of the respective cDNA, disclosed a high degree of homology (67% identity, 80% similarity) to the human 27-kD HSP. Southern blot of chicken genomic DNA disclosed one band, suggesting the presence of a single gene, and Northern blot analysis revealed abundant transcript of approximately 1 kb in gizzard and heart tissues and lower amounts in total 18-d chick embryo RNA and in cultured fibroblasts. Exposure of the latter cells to 45 degrees C resulted in over 15-fold increase in the apparent level of the 25-kD IAP protein, confirming that its expression is regulated by heat shock. Immunofluorescent microscopic localization indicated that after heat treatment, the levels of the 25-kD IAP were markedly increased and the protein was apparently associated with cytoplasmic granules. Heat shock also had a transient, yet prominent, effect on the microfilament system in cultured fibroblasts: stress fibers disintegrated within 10-15 min after incubation at 45 degrees C, yet upon further incubation at the elevated temperature, conspicuous actin bundles were apparently reformed.
448 citations
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TL;DR: The Drosophila gene, dorsal, is a maternal effect locus that is essential for the establishment of dorsal-ventral polarity in the developing embryo.
Abstract: The Drosophila gene, dorsal, is a maternal effect locus that is essential for the establishment of dorsal-ventral polarity in the developing embryo. The dorsal protein was predicted from the complementary DNA sequence; it is almost 50 percent identical, over an extensive region, to the protein encoded by the avian oncogene v-rel, its cellular homolog, c-rel, and a human c-rel fragment. The oncogene v-rel is highly oncogenic in avian lymphoid, spleen, and bone marrow cells.
448 citations