Topic
Complementary DNA
About: Complementary DNA is a research topic. Over the lifetime, 55301 publications have been published within this topic receiving 2752650 citations. The topic is also known as: cDNA & DNA, Complementary.
Papers published on a yearly basis
Papers
More filters
TL;DR: Comparison with partial sequences of a second C4A allele and a C4B allele has led to the tentative identification of some class differences in nucleotide sequences between C3A and C3B and of allelic differences between C4 a and B alleles in this highly polymorphic system.
378 citations
TL;DR: This work has identified 73 peroxidase genes throughout the Arabidopsis genome that have been investigated by analyzing the gene structure (intron/exon) in correlation with the phylogenetic relationships between the isoperoxidases and an evolutionary pattern of extensive gene duplications is inferred.
377 citations
TL;DR: Yeast strains lacking ATX1 are deficient in high affinity iron uptake and expression of HAH1 in these strains permits growth on iron-depleted media and results in restoration of copper incorporation into newly synthesized Fet3p.
377 citations
TL;DR: Alkaline phosphatase treatment of one Asp-N peptide converted it to a peptide of mass 673, demonstrating that this peptide contains the phosphate group, confirming that Ser3 in the encoded ADF is the single in vivo regulatory site.
377 citations
TL;DR: The results indicate that plant cells respond to changes in cell turgor by rapidly increasing transcription of several genes, which varies with respect to the time course of induction and reversibility of the wilting-induced changes.
Abstract: Reduction of turgor in pea shoots caused the accumulation of several poly(A) RNAs cDNA clones derived from three different poly(A) RNAs which accumulate in wilted pea shoots were isolated, sequenced and expression of the corresponding genes examined Clone 7a encoded a 289 amino acid protein The C-terminal 180 amino acids of this protein were homologous to soybean nodulin-26 RNA hybridizing to cDNA 7a was abundant in roots, and induced in shoots by dehydration, heat shock and to a small extent by ABA Hydropathic plots indicate that the protein encoded by cDNA 7a contains six potential membrane spanning domains similar to proteins which form ion channels Clone 15a encoded a 363 amino acid protein with high homology to cysteine proteases RNA hybridizing to cDNA 15a was more abundant in roots than shoots of control plants Dehydration of pea shoots induced cDNA 15a mRNA levels whereas heat shock or ABA treatment did not Clone 26g encoded a 508 amino acid protein with 30% residue identity to several aldehyde dehydrogenases RNA hybridizing to cDNA 26g was induced by dehydration of shoots but not roots and heat shock and ABA did not modulate RNA levels Levels of the three poly(A) RNAs increased 4–6-fold by 4 h after wilting and this increase was not altered by pretreatment of shoots with cycloheximide When wilted shoots were rehydrated, RNA hybridizing to cDNA 26g declined to pre-stress levels within 2 h Run-on transcription experiments using nuclei from pea shoots showed that transcription of the genes which encode the three poly(A) RNAs was induced within 30 min following reduction of shoot turgor One of the genes showed a further increase in transcription by 4 h after dehydration whereas transcription of the other 2 genes declined These results indicate that plant cells respond to changes in cell turgor by rapidly increasing transcription of several genes Furthermore, the expression of the turgor-responsive genes varies with respect to the time course of induction and reversibility of the wilting-induced changes
377 citations