Topic
Complementary DNA
About: Complementary DNA is a research topic. Over the lifetime, 55301 publications have been published within this topic receiving 2752650 citations. The topic is also known as: cDNA & DNA, Complementary.
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TL;DR: It is shown that RCCl specifically catalyses the exchange of guanine nucleo-tides on the Ran protein but not on the protein c-Ha-ras p21 (p21ras), and that the protein binds GDP and GTP.
Abstract: THE product of the gene RCC1 (regulator of chromosome condensation) in a BHK cell line is involved in the control of mitotic events1. Homologous genes have been found in Xenopus2, Drosophila3 and yeast4,5. A human genomic DNA fragment and complementary DNA that complement a temperature-sensitive mutation ofRCC1 in BHK21 cells6,7 encode a protein of relative molecular mass 45,000 (Mr45K) which is located in the nucleus and binds to chromatin8. We have recently isolated a protein from HeLa cells that strongly binds an anti-RCCl antibody and has the same molecular mass, DNA-binding properties, and amino-acid sequence as the 205 residues already identified9. HeLa cell RCCl is complexed to a protein of Mr 25K (ref. 9). We have shown10 that this 25K protein has a sequence homologous to the translated reading frame ofTC4, a cDNA found by screening a human teratocarcinoma cDNA library with oligonucleotides coding for a ras consensus sequence11, and that the protein binds GDP and GTP. We have referred to this protein as the Ran protein (ras-related nuclear protein). In addition to the fraction of Ran protein complexed to RCCl, a 25-fold molar excess of the protein over RCCl was found in the nucleoplasm of HeLa cells. Here we show that RCCl specifically catalyses the exchange of guanine nucleo-tides on the Ran protein but not on the protein c-Ha-ras p21 (p21ras).
669 citations
TL;DR: A human myelomonocytic cell line, U937, produced an interleukin-1 (IL-1) receptor antagonist protein (IRAP) which was purified and partially sequenced and was a potent inhibitor of IL-1 induced corticosterone production in vivo.
Abstract: A human myelomonocytic cell line, U937, produced an interleukin-1 (IL-1) receptor antagonist protein (IRAP) which was purified and partially sequenced. A complementary DNA coding for IRAP was cloned and sequenced. The mature translation product of the cDNA has been expressed in Escherichia coli and was an active competitive inhibitor of the binding of IL-1 to the T-cell/fibroblast form of the IL-1 receptor. Recombinant IRAP specifically inhibited IL-1 bioactivity on T cells and endothelial cells in vitro and was a potent inhibitor of IL-1 induced corticosterone production in vivo.
668 citations
TL;DR: A factor that promotes the growth of certain B cell hybridomas and of plasmacytomas is shown to be produced by normal human fibroblasts and by a line of human osteosarcoma cells after treatment with IL-1 or TNF.
Abstract: A factor that promotes the growth of certain B cell hybridomas and of plasmacytomas is shown to be produced by normal human fibroblasts and by a line of human osteosarcoma cells (MG-63) after treatment with IL-1 or TNF. The hybridoma-plasmacytoma growth factor (HPGF) is identified with a 26 kD protein whose mRNA was previously shown to be induced in the same cells by the same inducers. First, poly(A)-rich RNA extracted from IL-1-treated cells could be enriched in HPGF-mRNA content by hybridization to 26 kD cDNA. Second, MG-63-derived HPGF purified to electrophoretic homogeneity was subjected to amino acid sequence analysis, whereby the NH2-terminal sequence was found to match the nucleotide sequence of a 26 kD cDNA clone.
665 citations
TL;DR: The method was highly sensitive and could detect a single melanoma cell from a cell line in 2 ml normal blood, and could prove useful in the diagnosis of primary or metastatic cancers, in assessing prognosis, and in detecting residual disease after treatment.
665 citations
TL;DR: A membrane polypeptide involved in K+ transport in a higher plant was cloned by complementation of a yeast mutant defective in K+, with a complementary DNA library from Arabidopsis thaliana that conferred ability to grow on media with K+ concentration in the micromolar range and to absorb K+ (or 86Rb+) at rates similar to those in wild-type yeast.
Abstract: A membrane polypeptide involved in K+ transport in a higher plant was cloned by complementation of a yeast mutant defective in K+ uptake with a complementary DNA library from Arabidopsis thaliana. A 2.65-kilobase complementary DNA conferred ability to grow on media with K+ concentration in the micromolar range and to absorb K+ (or 86Rb+) at rates similar to those in wild-type yeast. The predicted amino acid sequence (838 amino acids) has three domains: a channel-forming region homologous to animal K+ channels, a cyclic nucleotide-binding site, and an ankyrin-like region.
665 citations