Topic
Complementary DNA
About: Complementary DNA is a research topic. Over the lifetime, 55301 publications have been published within this topic receiving 2752650 citations. The topic is also known as: cDNA & DNA, Complementary.
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TL;DR: A novel approach has been developed for the preparation of highly radioactive, strand-specific M13 probes by using a universal primer to initiate the DNA synthesis of the complementary strand of the M13 sequence downstream from the inserted sequence.
526 citations
TL;DR: This study indicates that ER alpha and ER beta can interact in vivo, cross-signaling each other.
526 citations
TL;DR: Eight classes of human leukocyte interferon cDNA clones have been identified in a cDNA library prepared from a myeloblastoid cell line and nucleotide sequences demonstrate that the multiple human LeIFN genes code for a family of homologous, yet distinct proteins.
Abstract: Eight classes of human leukocyte interferon (LeIFN) cDNA clones have been identified in a cDNA library prepared from a myeloblastoid cell line. The nucleotide sequences demonstrate that the multiple human LeIFN genes code for a family of homologous, yet distinct proteins. One of the cDNA clones may have been derived from the transcription of a LeIFN pseudogene.
524 citations
TL;DR: A human thyroid cDNA library with two synthetic oligonucleotides based on the reported amino acid sequence of the 3rd and 4th transmembrane domains of a putative human TSH receptor and related receptors generated a functional receptor, able to activate adenylate cyclase specifically in response to TSH stimulation.
524 citations
TL;DR: Results indicate that this human cDNA clone encodes a protein structurally and functionally homologous to mouse BSF-1, two properties of mouse B SF-1 on mouse cells.
Abstract: A cDNA sequence coding for a human interleukin has been isolated from a concanavalin A-activated human T-cell cDNA library based on homology with a mouse interleukin cDNA that expresses B-cell stimulatory factor 1 (BSF-1) activity and T-cell- and mast-cell-stimulating activities. The human cDNA contains a single open reading frame encoding a protein of 153 amino acid residues including a putative signal peptide. Amino acid sequences of the mouse and human polypeptides, deduced from their cDNAs, share extensive homology with the exception of about 40 amino acid residues near the middle portion, which share little homology. Supernatant of COS-7 monkey cells transfected with the human cDNA clone stimulated proliferation of human helper T-cell clones and of anti-IgM-activated human B cells, two properties of mouse BSF-1 on mouse cells. These results indicate that this human cDNA clone encodes a protein structurally and functionally homologous to mouse BSF-1.
523 citations