Topic
Contrast transfer function
About: Contrast transfer function is a research topic. Over the lifetime, 934 publications have been published within this topic receiving 26533 citations.
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TL;DR: Recently an electromagnetic hexapole system for the correction of the spherical aberration of the objective lens of a 200 kV transmission electron microscope has been constructed and new imaging modes are available, which can extend the point resolution of phase-contrast imaging to the information limit.
204 citations
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TL;DR: Adaptive aberration correction for depth‐induced spherical aberration in a multiphoton scanning microscope with a micromachined deformable mirror using a genetic learning algorithm with two‐photon fluorescence intensity feedback is demonstrated.
Abstract: Summary We demonstrate adaptive aberration correction for depthinduced spherical aberration in a multiphoton scanning microscope with a micromachined deformable mirror. Correction was made using a genetic learning algorithm with two-photon fluorescence intensity feedback to determine the desired shape for an adaptive mirror. For a 40 × /0.6 NA long working distance objective, the axial scanning range was increased from 150 mm to 600 mm.
202 citations
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189 citations
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TL;DR: A new three-dimensional model for the contrast transfer function is implemented in a regularized likelihood optimization algorithm as implemented in the RELION program, and it is shown that reducing radiation-induced specimen movement may be central to attempts at further improving tomogram quality and resolution.
186 citations
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TL;DR: In this article, the optical properties of a spherical aberration corrected transmission electron microscope by means of beam tilt series are demonstrated by measuring residual wave aberrations up to the fifth order.
181 citations