Coturnix

About: Coturnix is a research topic. Over the lifetime, 953 publications have been published within this topic receiving 23305 citations.

Rous sarcoma virus mutant dlPA105 induces different transformed phenotypes in quail embryonic fibroblasts and neuroretina cells.

Abstract: dlPA105 is a spontaneous variant of Rous sarcoma virus, subgroup E, which carries a deletion in the N-terminal portion of the v-src gene coding sequence. This virus was isolated on the basis of its ability to induce proliferation of quiescent quail neuroretina cells. The altered v-src gene encodes a phosphoprotein of 45,000 daltons which possesses tyrosine kinase activity. DNA sequencing of the mutant v-src gene has shown that deletion extends from amino acid 33 to 126 of wild-type p60v-src. We investigated the tumorigenic and transforming properties of this mutant virus. dlPA105 induced fibrosarcomas in quails with an incidence identical to that induced by wild-type virus. Quail neuroretina cells infected with the mutant virus were morphologically transformed and formed colonies in soft agar. In contrast, dlPA105 induced only limited morphological alterations in quail fibroblasts and was defective in promoting anchorage-independent growth of these cells. Synthesis and tyrosine kinase activity of the mutant p45v-src were similar in both cell types. These data indicate that the portion of the v-src protein deleted in p45v-src is dispensable for the mitogenic and tumorigenic properties of wild-type p60v-src, whereas it is required for in vitro transformation of fibroblasts. The ability of dlPA105 to induce different transformation phenotypes in quail fibroblasts and quail neuroretina cells is a property unique to this Rous sarcoma virus mutant and provides evidence for the existence of cell-type-specific response to v-src proteins.

[Transmission of Marek's disease to wild feathered game]

Abstract: The following wild feathered game were tested from 6 to 12 months of age: common pheasant (Phasianus colchicus), common gray partridge (Perdix perdix), European quail (Coturnix coturnix), and Greek partridge (Alectoris graeca). Only pheasant was found to be susceptible to infection with a virulent virus of Marek's disease. In this bird the first clinical symptoms of the disease (nervous changes) were observed in 28 days from infection. Immunoprecipitation antibodies to the Marek's disease virus occurred in the serum on the 75th day from infection, and patho-morphological changes, typical of acute Marek's disease, were observed on the 75th to 85th day from infection. The remaining species of wild feathered game appeared not to be susceptible to infection with the Marek's disease virus. In these birds, like in pheasants, immunoprecipitation antigens of the Marek's disease virus were not found to be present in the epithelial cells of feather follicles. In the infected Greek partridge, common gray partridge and quail, serum was not found to contain any antibodies. Laying quail had no antibodies in the yolk of the eggs they laid. The results indicate a different oncogenesis of Marek's disease in the domestic fowl and pheasant. It is concluded that wild feathered game play no important role in the epizootology of Marek's disease.

A Lewis X hapten is the major glycolipid of Japanese quail intestine.

Abstract: A fucolipid isolated from Japanese quail intestine was identified as beta-galactosyl-1,4-(alpha-fucosyl-1,3-) beta-N-acetylglucosaminyl-1,3-beta-galactosyl-1,4-beta-glucosyl-1,1-cera mide, a glycolipid which exhibits X-hapten activity. Analysis of the tissue at various embryonic stages showed that the fucolipid is characteristically present at later stages of organogenesis.