Topic
Coturnix
About: Coturnix is a research topic. Over the lifetime, 953 publications have been published within this topic receiving 23305 citations.
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TL;DR: The data suggest that the microchromosome-specific highly repetitive sequences of the blue-breasted quail and chicken were derived from a common ancestral sequence, and that they are one of the major and essential components of chromosomal heterochromatin in Galliformes species.
Abstract: A new family of centromeric highly repetitive DNA sequences was isolated from EcoRI-digested genomic DNA of the blue-breasted quail (Coturnix chinensis, Galliformes), and characterized by filter hybridization and chromosome in situ hybridization. The repeated elements were divided into two types by nucleotide length and chromosomal distribution; the 578-bp element predominantly localized to microchromosomes and the 1,524-bp element localized to chromosomes 1 and 2. The 578-bp element represented tandem arrays and did not hybridize to genomic DNAs of other Galliformes species, chicken (Gallus gallus), Japanese quail (Coturnix japonica) and guinea fowl (Numida meleagris). On the other hand, the 1,524-bp element was not organized in tandem arrays, and did hybridize to the genomic DNAs of three other Galliformes species, suggesting that the 1,524-bp element is highly conserved in the Galliformes. The 578-bp element was composed of basic 20-bp internal repeats, and the consensus nucleotide sequence of the internal repeats had homologies to the 41-42 bp CNM repeat and the XHOI family repeat of chicken. Our data suggest that the microchromosome-specific highly repetitive sequences of the blue-breasted quail and chicken were derived from a common ancestral sequence, and that they are one of the major and essential components of chromosomal heterochromatin in Galliformes species.
15 citations
01 Jan 2004
15 citations
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TL;DR: Converging data, including the facts that these cells divide in the brain mantle and express proliferating cell nuclear antigen (PCNA), a cell cycling marker, indicate that cells labeled by BrdU during the second half of embryonic life are slow‐cycling progenitors born and residing in thebrain mantle.
Abstract: Several brain areas in the diencephalon are involved in the activation and expression of sexual behavior, including in quail the medial preoptic nucleus (POM). However, the ontogeny of these diencephalic brain nuclei has not to this date been examined in detail. We investigated the ontogeny of POM and other steroid-sensitive brain regions by injecting quail eggs with 5-bromo-2-deoxyuridine (BrdU) at various stages between embryonic day (E)3 and E16 and killing animals at postnatal (PN) days 3 or 56. In the POM, large numbers of BrdU-positive cells were observed in subjects injected from E3-E10, the numbers of these cells was intermediate in birds injected on E12, and most cells were postmitotic in both sexes on E14-E16. Injections on E3-E4 labeled large numbers of Hu-positive cells in POM. In contrast, injections performed at a later stage labeled cells that do not express aromatase nor neuronal markers such as Hu or NeuN in the POM and other steroid-sensitive nuclei and thus do not have a neuronal phenotype in these locations, contrary to what is observed in the telencephalon and cerebellum. No evidence could also be collected to demonstrate that these cells have a glial nature. Converging data, including the facts that these cells divide in the brain mantle and express proliferating cell nuclear antigen (PCNA), a cell cycling marker, indicate that cells labeled by BrdU during the second half of embryonic life are slow-cycling progenitors born and residing in the brain mantle. Future research should now identify their functional significance.
15 citations
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TL;DR: A new approach, based on high-resolution melting (HRM) curve analysis of the CHD1 gene, for avian gender identification, was successfully applied to carry out sexual differentiation based on melting curve patterns in common quail and Japanese quail.
Abstract: Sex identification in birds through molecular methods is an important tool for the management and preservation of species. Advances in real-time PCR-based techniques overcome some limitations of the more classical molecular analysis methodologies. Here, we describe a new approach, based on high-resolution melting (HRM) curve analysis of the CHD1 gene, for avian gender identification. This method was successfully applied to carry out sexual differentiation based on melting curve patterns in common quail (Coturnix c. coturnix) and Japanese quail (Coturnix c. japonica). We clearly demonstrate the efficacy of a simple HRM assay for a rapid and efficient gender differentiation of these subspecies and propose this methodology as a valuable addition to expand the applicability of real-time PCR-based technology in avian molecular sexing.
15 citations
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TL;DR: Sequences related to the avian retrovirus Rous sarcoma virus within the genome of the Japanese quail, a species previously considered to be free of endogenous avian leukosis virus elements, are detected, consistent with the presence of an endogenous retroviral element within the quail genome.
Abstract: We detected sequences related to the avian retrovirus Rous sarcoma virus within the genome of the Japanese quail, a species previously considered to be free of endogenous avian leukosis virus elements Using low-stringency conditions of hybridization, we screened a quail genomic library for clones containing retrovirus-related information Of five clones so selected, one, lambda Q48, contained sequence information related to the gag, pol, and env genes of Rous sarcoma virus arranged in a contiguous fashion and spanning a distance of approximately 58 kilobases This organization is consistent with the presence of an endogenous retroviral element within the Japanese quail genome Use of this element as a high-stringency probe on Southern blots of genomic digests of several quail DNA demonstrated hybridization to a series of high-molecular-weight bands By slot hybridization to quail DNA with a cloned probe, it was deduced that there were approximately 300 copies per diploid cell In addition, the quail element also hybridized at low stringency to the DNA of the White Leghorn chicken and at high stringency to the DNAs of several species of jungle fowl and both true and ruffed pheasants Limited nucleotide sequencing analysis of lambda Q48 revealed homologies of 65, 52, and 46% compared with the sequence of Rous sarcoma virus strain Prague C for the endonuclease domain of pol, the pol-env junction, and the 3'-terminal region of env, respectively Comparisons at the amino acid level were also significant, thus confirming the retrovirus relatedness of the cloned quail element
15 citations