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Cyclase
About: Cyclase is a research topic. Over the lifetime, 10162 publications have been published within this topic receiving 388566 citations.
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TL;DR: In Y1(Cyc) mutants, corticotropin had little effect on steroidogenesis, whereas cyclic nucleotides were fully active, which implies that adenylate cyclase and cyclic AMP-dependent protein kinase are obligatory components of the corticotropic-stimulated steroidogenic pathway.
Abstract: Two groups of mutant clones were isolated from YI adrenocortical tumor cells. One group, Y1(Kin), exhibited altered cytosolic cyclic AMP-dependent protein kinase activity; the second group, Y1(Cyc), exhibited diminished corticotropin-responsive adenylate cyclase activity. Steroidogenic responses to corticotropin and cyclic nucleotides closely paralleled cyclic AMP-dependent protein kinase activity in the Y1(Kin) mutants. In Y1(Cyc) mutants, corticotropin had little effect on steroidogenesis, whereas cyclic nucleotides were fully active. These data imply that adenylate cyclase and cyclic AMP-dependent protein kinase are obligatory components of the corticotropin-stimulated steroidogenic pathway.
138 citations
TL;DR: It is shown here that colchicine, vinblastine and cis- unsaturated fatty acids enhance G unit-mediated activation of adenylate cyclase, implying that microtubules or tubulin are involved in the attachment of the G unit to the membrane.
Abstract: The adenylate cyclase system, which mediates cellular responses to a variety of hormones and neurotransmitters, contains at least three plasma membrane-associated proteins: the hormone receptor, the regulatory or guanyl nucleotide-binding unit (G unit) and the catalytic moiety. Activation of the enzyme after binding of hormone to the receptor involves binding of GTP to the G unit1. Activation can be enhanced by increasing the fluidity of the membrane with unsaturated fatty acids2 with the local anaesthetic prilocaine3, and perhaps, in vivo, by phospholipid methylation4. Disruption of microtubules increases localized mobility of membrane proteins in a manner similar to that of unsaturated fatty acids5 and can enhance cyclic AMP accumulation in intact leukocytes6–9. We therefore decided to investigate whether there is a direct interaction between micro-tubules and adenylate cyclase. We show here that colchicine, vinblastine and cis- unsaturated fatty acids enhance G unit-mediated activation of adenylate cyclase, implying that microtubules or tubulin are involved in the attachment of the G unit to the membrane.
137 citations
TL;DR: A membrane form of guanylate cyclase represents a new model for cell surface receptors, although such a model was once envisioned for adenylate cyclase.
137 citations
TL;DR: The requirement for stereospecificity for the interaction of this component with the cannabinoid receptor, defined by the binding of [3H]CP-55,940 and the adenylate cyclase enzyme, was examined.
137 citations
TL;DR: Sixty-two spontaneous mutations have been characterized which reduce the level of expression of catabolite-sensitive operons in cya mutants and appear to affect either the crp or cya loci.
Abstract: Sixty-two spontaneous mutations have been characterized which reduce the level of expression of catabolite-sensitive operons. These mutations appear to affect either the crp (catabolite gene activator protein) or cya (adenyl cyclase) loci. No new loci have been discovered. Deletions of the cya gene do not remove an essential function. φ80 transducing phage for the cya gene have been used to do recombination and complementation studies on cya mutants.
137 citations